LOCUS       sequence-34568-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are 
            expressed as a single poly-cistronic unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-C5
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..103)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cttgctgcgg gcccggcggc ttcagctccg tctccatcat gttatacatt gggccaggat
       61 tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctcctccg gacccgccgc
      121 cgtttgaatg catgggagag cccagagcag tgacgggaac agagggaaag gcctcgccct
      181 caggaaaagg gactgagtag agtgtggtga agtgggggct tccatagcct ggggtgccaa
      241 agtggggacc tgggggcaga ggaaaggata cagccccccc tgggaaaggt gtccctgtag
      301 cctcatactc ttctcgttgg gaatactcaa tacttgatct tttgcccttc tggcgccggt
      361 tacagaacca tactcgaacc acatccttct ctagcccaag ctgattggcg atgtgagtga
      421 tctgctgtag ggagggcttc gggcacttca gaaacatggt ctccanactc cacctcacac
      481 ggttctcaat gctagttcgc tttctcttcc gggcctgcac cagggtctcc gatttgcata
      541 tctcctgaag gttctcattg ttgtcggctt cctccaccca cttctccagc aggggccgca
//
