LOCUS       sequence-345680-        5636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0793:Dendra
FEATURES             Location/Qualifiers
     source          1..5636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1218..1237
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1218..1236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2752..2775
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3032..3832
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3957..4545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4446..4465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
       61 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
      121 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
      181 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
      241 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
      301 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
      361 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
      421 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
      481 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
      541 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
      601 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
      661 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
      721 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
      781 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
      841 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
      901 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
      961 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     1021 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     1081 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     1141 ggtctctgac gagcgggaga actccctatc agtgatagag tttgtcctcc ctatcagtga
     1201 tagataggct taaggagtaa tacgactcac tatagggaga aggaggccat tatggctgac
     1261 tccgtactgc gcatcggggg gcgcgaattc ggctcccggc tgatcatggg caccggcggt
     1321 gcacccaacc tctcggtgct cgaagaggcg ctcatcgcct caggcaccga actgaccacc
     1381 gtcgcgatgc gacgcgtcga cgccgaaacc ggaacgggcg tactcgatct gctcaaccgg
     1441 ttgggcatcg ccgcgctgcc caacaccgcc ggctgccgcg gcgcggccga ggcggtgctc
     1501 accgcgcagc tcgcgcgtga ggcgctcggg accgacatgg tgaaactcga ggtcatcgcc
     1561 gacgagcgca ccctgctgcc cgacgcggtc gaactcgtca aggccgcaga acaattggtc
     1621 gacgacgggt tcaccgtgct gccctacacc aacgacgacc cggtgctggc gcgtcggctc
     1681 gaggacatcg gatgtgccgc ggtgatgccg ctcggctcgc cgatcggcac cgggctgggc
     1741 atctccaacc cgcacaacat cgagatgatc gtcgcggccg cgggggtgcc cgtggtgctc
     1801 gacgccggta tcggcaccgc gagcgacgcc gcgctggcga tggaactggg ttgtgacgcc
     1861 gtgcttttgg ccactgccgt cacacgcgcg tccgatccgc cgaccatggc cgccgcgatg
     1921 gcctcggccg tcaccgctgg ccatctcgcc cggcaggcgg gtcgtatccc taagcggttc
     1981 tgggcgcagg cgtcgagccc ggcgctgaag cttggtgcgg ccgcgggcaa cacgcctggc
     2041 atcaatctga tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt
     2101 cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc
     2161 aacctcaccg tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg
     2221 gtgcattacg ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag
     2281 cagagcttcc cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc
     2341 tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc
     2401 aaggggacca actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag
     2461 ccgagcacgg agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg
     2521 ctgctgctgg aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag
     2581 aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac
     2641 gattcggact acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc
     2701 ccgtcgcagg tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag
     2761 gacgacgacg acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt
     2821 tttttgttaa ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg
     2881 cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg
     2941 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
     3001 acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt
     3061 gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc
     3121 cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc
     3181 acttggactg atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc
     3241 ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga
     3301 ccttggagtt gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc
     3361 atttgccttt gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct
     3421 gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact
     3481 tctcctcggc gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa
     3541 ggttccctat ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt
     3601 cgattatctc gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa
     3661 ggagaagagc cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc
     3721 ccgcgacatt gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca
     3781 tccgccagag gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca
     3841 tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg
     3901 ggggatccac tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4081 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga
     4381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4681 agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac
     4741 caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc
     4801 cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc
     4861 cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg
     4921 cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca
     4981 gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag
     5041 acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga
     5101 cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc
     5161 cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag
     5221 gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt
     5281 cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca
     5341 gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc
     5401 agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg
     5461 agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca
     5521 cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg
     5581 atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcg
//
