LOCUS       sequence-345682-        5720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1345:Dendra
FEATURES             Location/Qualifiers
     source          1..5720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1976..2564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2056..2075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2689..3489)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(3746..3769)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5368..5387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5369..5387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcggcggt gctggcgctc gcggccaggc gtggttcttc gcgtactgac aagaaacccc
       61 cctctttagg actcagtgtc cttgggaggg gggctttttg cgtttcagga ggtcttggcc
      121 agcttggaca tcgcctcagc gatagcctcg tcgcgggcct cagacgccat ctggtacttc
      181 atcgccatcc taggagtcgt gtgaccgaga cgggccatca gctccttggt cgtcgcacct
      241 gcctgagcgg cgaacgtagc gccgacagcg cggaggtcgt ggatgcggag ttccggccga
      301 ccgatcttgg cgtagccacg cttcagcgac ttggtgaacg cggacttcga cagccggttg
      361 ccctgcgtcg tggtcaccag gaatgcctcg gggcccttgt tcatcttcgt acggtccttc
      421 atgtgcgctc ggatcatctc cgcgacgtga ggcggaaccg tcacaggacg cttcgaccgg
      481 acggtcttgg cgttgccaac gacgatcttg ttccccacgc gggaagcgcc acggcgcacc
      541 cggagcttca tcgtcatgcc gtcgtccacg atgtccttgc ggcgaagctc gatcagctct
      601 ccgaaccgga ggctcgtcca cgccaggatg tatgccgcga tccggtagtg ctcgaagatc
      661 tcagcggcga cgatgtccag ctcctcaggc gtcagcgcct ctacgtcgcg ctcatcggct
      721 gccttctgct cgatccggca cgggttctct gcgatcagct tgtcctcgac cgctgtgttc
      781 atcaccgccc ggaggacgtt gtaggcatgc cggcgggcag tcgggtgctt cctacccatc
      841 ccggcccacc acgcacgcac cagagctggc gtcatctctg tgaccgccac ttcacctagc
      901 accgggtaga tgcggcgctc cgcgtgcccg ctgtacagat ccctggtgcc gtctgcgagg
      961 tcgcgctcca cgagccactt ccgggtgtac tcctccagcg tgatggcgct ggcggctgcc
     1021 ttcttcgccc ggtcctgtgg aggggtccag gtctccatct cgatgagccg cttctcgccc
     1081 gcgagccagg cttcggcgtc catcttgttg tcgtaggtct gcagcgcgta gtacctcaca
     1141 ccgtcctgcg ggttgacgta tgaggcttgg atcctcccgc tgcgctgagt cttcagcgat
     1201 ccccatccgc gacgtgccaa ctaggtctcc tctcgtcgtg aacaaggcta ccgggttgca
     1261 actcctgtgc aactctcagg cttcaacgcg cttctacgac ctgcaatttc tttccactta
     1321 gaggatgcag ccgagagggg gtaaaaacct atcttgaccg gcccatatgt ggtcggcaga
     1381 cacccattct tccaaactag ctacgcgggt tcgattcccg tcgcccgctc cgctggtcag
     1441 agggtgtttt cgccctctgg ccatttttct ttccaggggt ctgcaactct tgtgcgactc
     1501 ttctgacctg ggcatacgcg gttgcaacgc atccctgatc tggctacttt cgatgctgac
     1561 aaacgaatag agccccccgc ctgcgcgaac agacgagggg cattcacacc agattggagc
     1621 tggtgcagtg aagagaatag accgggacaa ggttgcaccg ggagttgcag cggtcggaac
     1681 cctcgccgtc ggcgggctgg cgttcgccct gtcgttcacg gctctcagcg agctggctgc
     1741 ggccaacggg gtggcccaag cagagatggt gcccttggtg gtcgactcta gaggatcccc
     1801 gggtaccgcg gccgcacgcg ttgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccctcg ggaagcgtgg
     2161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2401 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2581 tttctacggg gtctgacgct catggaacta gtggatcccc cgtgttgccc cagcaatcag
     2641 cgcgaccttg cccctccaac gtcatctcgt tctccgctca tgagctcagc caatcgactg
     2701 gcgagcggca tcgcattctt cgcatcccgc ctctggcgga tgcaggaaga tcaacggatc
     2761 tcggcccagt tgacccaggg ctgtcgccac aatgtcgcgg gagcggatca accgagcaaa
     2821 ggcatgaccg actggacctt ccttctgaag gctcttctcc ttgagccacc tgtccgccaa
     2881 ggcaaagcgc tcacagcagt ggtcattctc gagataatcg acgcgtacca acttgccatc
     2941 ctgaagaatg gtgcagtgtc tcggcacccc atagggaacc tttgccatca actcggcaag
     3001 atgcagcgtc gtgttggcat cgtgtcccac gccgaggaga agtacctgcc catcgagttc
     3061 atggacacgg gcgaccgggc ttgcaggcga gtaaggtggc aggggcaatg gatcagagat
     3121 gatctgctct gcctgtggcc ccgctgccgc aaaggcaaat ggatgggcgc tgcgctttac
     3181 atttggcagg cgccagaatg tgtcagagac aactccaagg tccggtgtaa cgggcgacgt
     3241 ggcaggatcg aacggctcgt cgtccagacc tgaccacgag ggcatgacga gcgtccctcc
     3301 cggacccagc gtagcacgca gggcctcgat cagtccaagt ggcccatctt cgaggggccg
     3361 gacgctacgg aaggagctgt ggaccagcag cacaccgccg ggggtaaccc caaggttgag
     3421 aagctgaccg atgagctcgg cttttcgcca ttcgtattgc acgacattgc actccaccgc
     3481 tgatgacatc agtcgatcat agcacgatca acggcactgt tgcaaatagt cggtggtgat
     3541 aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa ggccggcatt
     3601 ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc atcagttacc
     3661 gtgagctgca ggaattcgat cgtacgctag ttaacaaaaa acccctcaag acccgtttag
     3721 aggccccaag gggttattta aatcacttgt cgtcgtcgtc cttgtagtcc tggaaataca
     3781 ggttctcccc gccgccgcct tcgaaccaga cctgcgacgg gagcgggcta tagcgtgcca
     3841 cagcgtgctc gtacaacttg accttgttgt agtccgaatc gttccccagg atttcgatgc
     3901 ggtggtccac gaagtgcgcg tcgggcagct ggacgacctt cttcgctttg tacgtggtct
     3961 tgaagtcgca taggtagtgg ccgccgcctt ccagcagcag cgccatgttg atgttgccga
     4021 ccaggaggcc gtcccgaacg tgcagcttct ccgtgctcgg ctcccacttg agggtcttct
     4081 tctgcatgac cgggccgttc ggcggaaagt tggtcccctt gaaacggacg ttctggaaga
     4141 aacagtcccc ttccagcgag atgtcgctgc ggatcgtaca gatacccttg tcttcgaagg
     4201 tcatcgtccg ctcccaggag tatccctccg ggaagctctg cttgaagtag tcagggatat
     4261 cctcggggta cttggtgaac acgcggttcc cgtaatgcac cgcggtagtg aggatatcgt
     4321 acgagaatgg gagaggggcg ccctccttga cggtgaggtt ggcggtctgg gtcccttcgt
     4381 aaggcttacc cttaccctcc ccctcgatca cgaaggcgtg accgttcacg ttgccctcca
     4441 tatgcacctt gacacgcatg tcctccttga tcagattgat gccaggcgtg ttgcccgcgg
     4501 ccgcaccaag cttaatcttt gcgacctggt tgaaggtcag ttcgaccggc gtctcgcgac
     4561 cgaagatcga caccagcacc ttgagcttct gctgctcggc gttgacctcg ctgatcgagg
     4621 cgggcagcgt cgcgaacggg ccgtccatga cggtgaccga ctcgccgacc tcgaagtcga
     4681 cgaggatctc gggacgctcc agcgtggcct cggtggaggc ggcggacgca gcggcggccg
     4741 cggccttgcc gggcttcttc gccgcgccct gcggcagcag gaacttgacg acgtcgtcga
     4801 gcgacagcgg cgacggacgc gaggtggcgc cgacgaatcc ggtcacaccc ggggtgttgc
     4861 gcaccgcgcc ccacgactcg tcgttgagct ccatgcgcac caggatgtag cccggcagca
     4921 ccttgcggtt gacctgcttg cgctggccgt tcttgatctc ggtgacctct tcggtgggca
     4981 cctcgacctg gaagatgtag tcgcccacat ccaggttctg cacgcgggtc tcgaggttgg
     5041 ccttcacctt gttctcgtaa ccggcgtagg agtggatgac gtaccagtcg ccgggcttga
     5101 ggcgcagctc cttcttgagc gcgacggccg ggtcctcatc ctcgtcggcg ggagcggcct
     5161 cgccctcgcc ctccgaagat tcgacaccct ccgaagcctc ggcagcctcg ccggcctcct
     5221 cggcagcgtc ggcgccctcg gtttcggccg cgtcagactc aacggcctca gactcggtgg
     5281 tctcggcagc ttcgacggcc tcagactcag cggtctcgtc ggcgaacgtc tcgtcgccgt
     5341 cgaacgtggt cataatggcc tccttctccc tatagtgagt cgtattactc cttaagccta
     5401 tctatcactg atagggagga caaactctat cactgatagg gagttctccc gctcgtcaga
     5461 gacccttctc acaccgggcc tgtgcgtgcg aacgcacaga tcaacgccgg gatggcgtag
     5521 agctcactgc gcagcaccgt ggggatgcgg ccgatcatca cgtcgcggat cgtgccgcct
     5581 cccacggccg tgacggtgcc gacgatccaa tatttcgacg tccccgatca aagcgggcgc
     5641 gttgtggtca ccgtctagat ttctggcggg aacggcaatg ctcctggcgg cggcggggct
     5701 ggtgcccagg tctccgcaca
//
