LOCUS       sequence-345703-        3250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD
            from the E coli RNAP with a SYNZIP17 domain fused to the N 
            terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Villegas Kcam MC, Tsong AJ, Chappell J
  TITLE     Rational engineering of a modular bacterial CRISPR-Cas activation 
            platform with expanded target range.
  JOURNAL   Nucleic Acids Res. 2021 May 7;49(8):4793-4802. doi: 
            10.1093/nar/gkab211.
  PUBMED    33823546
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-05-7- 7"; volume: "49"; issue: "8";
            pages: "4793-4802"
COMMENT     Sequence Label: pJEC626
FEATURES             Location/Qualifiers
     source          1..3250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..739)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        798..832
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1768..1797
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1813..1830
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1886..1903)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2056..2102
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2320..3111)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3112..3203)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
       61 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc catcctgcca
      121 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
      181 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
      241 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
      301 tctcccttac gggaagtgtg gcgctttctc atagctaaca cactggtatc tcggctcggt
      361 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
      421 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
      481 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
      541 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
      601 tgcgaaagcc agttaccacg gttaagcagt tcaccaactg acttaacctt cgatcaaacc
      661 acctcccaag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
      721 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
      781 ttcaaatgta gcacctgttt acggctagct cagtcctagg tatagtgcta gccgcttcta
      841 gagtcacaca ggaaagtact agatgcaggg ttctgtgaca gagtttctaa aaccgcgcct
      901 ggttgatatc gagcaagtga gttcgacgca cgccaaggtg acccttgagc ctttagagcg
      961 tggctttggc catactctgg gtaacgcact gcgccgtatt ctgctctcat cgatgccggg
     1021 ttgcgcggtg accgaggttg agattgatgg tgtactacat gagtacagca ccaaagaagg
     1081 cgttcaggaa gatatcctgg aaatcctgct caacctgaaa gggctggcgg tgagagttca
     1141 gggcaaagat gaagttattc ttaccttgaa taaatctggc attggccctg tgactgcagc
     1201 cgatatcacc cacgacggtg atgtcgaaat cgtcaagccg cagcacgtga tctgccacct
     1261 gaccgatgag aacgcgtcta ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt
     1321 gccggcttct acccgaattc attcggaaga agatgagcgc ccaatcggcc gtctgctggt
     1381 cgacgcatgc tacagccctg tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga
     1441 acagcgtacc gacctggaca agctggtcat cgaaatggaa accaacggca caatcgatcc
     1501 tgaagaggcg attcgtcgtg cggcaaccat tctggctgaa caactggaag ctttcgttga
     1561 cttacgtgat gtacgtcagc ctgaagtgaa agaagagaaa ccagagggag gttctggagg
     1621 tggtgggtcc atgaacgaaa aagaagaact gaaatctaaa aaagcggaac tgcgcaaccg
     1681 catcgaacag ctgaaacaga aacgcgaaca gctgaaacag aaaatcgcga acctgcgcaa
     1741 agaaatcgaa gcgtataaat aatgaccgaa caaaaactca tctcagaaga ggatctgaat
     1801 agcgccgtcg accatcatca tcatcatcat tgagtttaaa cggtctccag cttggctgtt
     1861 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     1921 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac ccaatgccga
     1981 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     2041 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2101 atctgttgtt tgtcggtgaa ctggatcctt actcgagtct agactgcagc tgaaacctca
     2161 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     2221 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc
     2281 ggatcttgcg gccactcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
     2341 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     2401 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     2461 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     2521 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     2581 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     2641 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     2701 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     2761 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     2821 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     2881 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     2941 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttaaggc cgccatccac
     3001 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     3061 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct
     3121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3181 atgtatttag aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag
     3241 actgcggcgg
//
