LOCUS       sequence-345715-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orthogonal Mb tRNA and tRNA synthetase for 
            incorporation of tetrazine3.0 in eukaryotic cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA
  TITLE     Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine 
            Amino Acids.
  JOURNAL   J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 
            10.1021/jacs.9b11520. Epub 2020 Apr 10.
  PUBMED    32251579
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.9b11520"; journalName: "J Am Chem Soc"; date: 
            "2020-04-22- 22"; volume: "142"; issue: "16"; pages: "7245-7249"
COMMENT     Sequence Label: pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(4142..4236)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4170..4188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4313..4561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    4598..4822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4811..4828
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4877..5465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4983..4994
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5392..5412
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6748..6771)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6775..6801)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      6822..6831
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6850..6869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6851..6869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6914..7117)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6943..6963)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7118..7497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7592..7683
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     7608..7625
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(7742..7789,7790..9277)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    9423..9557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9460..9479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9514..9533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9590..9838
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        9915..10009
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    9963..9981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9991..10009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     mobile_element  10102..10267
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc gcttaatgcg
     4021 ccgctacagg gcgcgtgggg atacccccta gagccccagc tgaaaaaacg gggggtgcgg
     4081 gagtttcacc cgcctgcacg gatttagagt ccgtgtgatc tattcgatcc accccccggt
     4141 gtctctatca ctgataggga acttataagt ctctatcact gatagggatt tcacgtttat
     4201 ggtgatttcc cagaacacat agcgacatgc aaatattaaa aaacggaaac cccgggaatc
     4261 taacccggct gaacggattt agagtccgtt cgatctacat gatcaggttt ccggtgtttc
     4321 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
     4381 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
     4441 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
     4501 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
     4561 cttcctgccc gagatctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca
     4621 gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc
     4681 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg
     4741 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat
     4801 ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac
     4861 tcgagttaaa gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     4921 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     4981 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     5041 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     5101 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     5161 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     5221 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     5281 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     5341 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     5401 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     5461 tgattgtcga cttaacgcgt tgaattctca tcacaggttg gtgctgatgc cgttgtagta
     5521 gctctcgctt ctgctggccc gcttgatgtt cttgaagccg tgcatcactt tcagcagccg
     5581 ttccaggccg aagccggctc cgatccaggg cttgtcgatg ccccactccc ggtccaggga
     5641 cacgggtccc accacggcgc tgctcagttc caggtcgccg tgcatgatgt ccagggtgtc
     5701 gccgtacacc atgcagctgt cgcccacgat ctcgaagtcg atttccaggt aatccaggaa
     5761 ctctttgatc agggcttcca ggttctcccg ggtgcagccg ctgcccatct ggctaaagcc
     5821 caccattgta aactcttcca ggtgctcttt gccgtcgctc tctttccggt agcagggtcc
     5881 cacttcgaaa atcttgatgg ggccaggcag gattctgtcc agtttccgca ggtagttgta
     5941 gccggtgggg gccagcatag gccgcaggca caggttcttg tccacccgga aaatctgctt
     6001 ggacagctcg gtgtcgttgt tgatgcccat ccgctccacg tactcggcgg ggatcaggat
     6061 ggggctcttg atttccagga agccccggtc cacgaagaac ttggtgatgt cccgttccag
     6121 cttgcccagg tagtcctccc ggtcgttggt gtacagccgc tggaagtcgt tcttccgccg
     6181 ggtcaccagc tcgggttcca gctcccggaa gggcttggcc atgttcaggc tgatcttgtc
     6241 ctcgggggac agcagggcct ccactctgtc cagctgggac cgtgtcaggc tgggagcagg
     6301 agcagaggcg ggcacggagc tgttgggggt gctcttggcg gggctgggca cgctgcggct
     6361 ggtgttggtg ctggccttgg cggacacgct gttttccagg ggcttggggg ctctggacac
     6421 gctcttgggc atggctttct tcactttggg ggcggacacc acccgcactt tcacgctgtt
     6481 cttgctctcg gtgcttctgg tcagaaagtt gttgatgtcc tcgtcggaca cccggcaccg
     6541 cttacaggtc tttctgtact tgtggtgccg gaaggctctg gcggtccggc agcttctgct
     6601 gttgttcacc accaggtggt cgccgcaggc catctcgatg tagattttgc ttcttgacac
     6661 ctcgtggtgc ttgatcttgt gcagggtgcc ggtccggctc atccacaggc cggtggcgct
     6721 gatcagcacg tccaggggtt tcttgtcctt gtcgtcgtcg tccttgtagt cgtcgagtgt
     6781 gaggcgctcc agtggaggca actgcaaagg aaccggacac gccatggtgg cgctagccag
     6841 cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc
     6901 tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     6961 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     7021 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     7081 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     7141 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     7201 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     7261 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     7321 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     7381 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     7441 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     7501 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg gatcctgcag gtatttgcgg
     7561 ccgcggtccg tatactccgg aatattaata gatcatggag ataattaaaa tgataaccat
     7621 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
     7681 aatattccgg attattcata ccgtcccacc atcgggcgcg aactcctaaa aaaccgccac
     7741 catgaagtgc cttttgtact tagccttttt attcattggg gtgaattgca agttcaccat
     7801 agtttttcca cacaaccaaa aaggaaactg gaaaaatgtt ccttctaatt accattattg
     7861 cccgtcaagc tcagatttaa attggcataa tgacttaata ggcacagcct tacaagtcaa
     7921 aatgcccaag agtcacaagg ctattcaagc agacggttgg atgtgtcatg cttccaaatg
     7981 ggtcactact tgtgatttcc gctggtatgg accgaagtat ataacacatt ccatccgatc
     8041 cttcactcca tctgtagaac aatgcaagga aagcattgaa caaacgaaac aaggaacttg
     8101 gctgaatcca ggcttccctc ctcaaagttg tggatatgca actgtgacgg atgccgaagc
     8161 agtgattgtc caggtgactc ctcaccatgt gctggttgat gaatacacag gagaatgggt
     8221 tgattcacag ttcatcaacg gaaaatgcag caattacata tgccccactg tccataactc
     8281 tacaacctgg cattctgact ataaggtcaa agggctatgt gattctaacc tcatttccat
     8341 ggacatcacc ttcttctcag aggacggaga gctatcatcc ctgggaaagg agggcacagg
     8401 gttcagaagt aactactttg cttatgaaac tggaggcaag gcctgcaaaa tgcaatactg
     8461 caagcattgg ggagtcagac tcccatcagg tgtctggttc gagatggctg ataaggatct
     8521 ctttgctgca gccagattcc ctgaatgccc agaagggtca agtatctctg ctccatctca
     8581 gacctcagtg gatgtaagtc taattcagga cgttgagagg atcttggatt attccctctg
     8641 ccaagaaacc tggagcaaaa tcagagcggg tcttccaatc tctccagtgg atctcagcta
     8701 tcttgctcct aaaaacccag gaaccggtcc tgctttcacc ataatcaatg gtaccctaaa
     8761 atactttgag accagataca tcagagtcga tattgctgct ccaatcctct caagaatggt
     8821 cggaatgatc agtggaacta ccacagaaag ggaactgtgg gatgactggg caccatatga
     8881 agacgtggaa attggaccca atggagttct gaggaccagt tcaggatata agtttccttt
     8941 atacatgatt ggacatggta tgttggactc cgatcttcat cttagctcaa aggctcaggt
     9001 gttcgaacat cctcacattc aagacgctgc ttcgcaactt cctgatgatg agagtttatt
     9061 ttttggtgat actgggctat ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag
     9121 ttggaaaagc tctattgcct cttttttctt tatcataggg ttaatcattg gactattctt
     9181 ggttctccga gttggtatcc atctttgcat taaattaaag cacaccaaga aaagacagat
     9241 ttatacagac atagagatga accgacttgg aaagtgataa ggccaggccg gccaagcttg
     9301 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     9361 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     9421 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9481 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9541 cttatcatgt ctggatctga tcactgcttg agcctaggtc gggcaggaag agggcctatt
     9601 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     9661 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     9721 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     9781 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
     9841 aaacctgatc atgtagatcg aacggactct aaatccgttc agccgggtta gattcccggg
     9901 gtttccgttt tttaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga
     9961 aatccctatc agtgatagag acttataagt tccctatcag tgatagagac accggggggt
    10021 ggatcgaata gatcacacgg actctaaatc cgtgcaggcg ggtgaaactc ccgcaccccc
    10081 cgttttttgc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
    10141 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
    10201 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
    10261 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
    10321 tgtgacaaac cgtcatcttc ggctacttt
//
