LOCUS       sequence-345727-       11534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSOUP helper plasmid needed for Agrobacterium-mediated plant 
            transformation - Expression of GOXL genes tagged with Citrine under 
            RUBY promoter for transgene complementation of ruby-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    656..678
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(702..954)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(974..1525)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1567..1746)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1978..2000
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1992..2009
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1993..2009
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2019..2038
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2019..2037
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8261..8277)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8261..8277)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(8300..8302,8303..9013)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8342..8363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8603..8622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8950..8971
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      9055..9307
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9326..9346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9326..9344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9365..9381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9365..9381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9378..9400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9389..9405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9413..9419,9420..9437,9438..9443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9458..9479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9596..9613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9718..9742
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9893..10328
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(10608..10627)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10619..11434
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             10619..11434
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10692..10711)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatga gatcttggca
      661 ggatatattg tggtgtaacg ttatcagctt gcatgccggt cgatctagta acatagatga
      721 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      781 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      841 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      901 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg
      961 actctagggg tcatcagatt tcggtgacgg gcaggaccgg acggggcggc accggcaggc
     1021 tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc
     1081 gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg
     1141 gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt
     1201 gggtgtagag cgtggagccc agtcccgtcc gctggtggcg gggggagacg tacacggtcg
     1261 actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg acccgcgtag gcgatgccgg
     1321 cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt
     1381 cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga
     1441 tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt
     1501 cggccgggcg tcgttctggg ctcatggtag atcccctcga tcgagttgag agtgaatatg
     1561 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     1621 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     1681 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     1741 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     1801 gtgactccct taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc
     1861 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     1921 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     1981 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     2041 aattgggtac cgctaaccca atctcaatcg aaccggacca aattagaatg aaatggtttt
     2101 agtgagttac atggttctta atttgactgg atgaaattaa ttaaaggcac tatatagatg
     2161 aaactaaaac tacaagaaca ttgctctaac ttttacacaa ctttaaaaaa aaaaaaaaaa
     2221 aaaatcgtgg aatgttgatt catttgacct ttattctgga ttgaaagtga tgcaaaaatg
     2281 actttatcta ataaataaaa aaaagaagac tttatctgat gtagtgaaat tcttgtattt
     2341 gttgatgcaa tgatgctatg gattgttttc aaatgtttca attacgtatc aaattatttt
     2401 atttgtacca acacatgcaa gtatgcaacc atggcctcgt cgtaaagaac tttgggcaac
     2461 atacaaattt gggcaaacat ctataacaca ttggattttt ttgatcgatt taatttgatt
     2521 cttggaatta ctattacctt cttcttttaa aaattcagta agatatgtgt taccgtgtga
     2581 aagtctatat gtgtgggaac gaaaggtttg aagacgagta agaaagtaga agagcacaat
     2641 gcgaaacttt attggtatac gtaactgttt attgacgaca tttgtaggaa caaaaattgc
     2701 aagataacac attttttagt gtttgctttg cttgtttata gtaaccggta ggcagtggcg
     2761 gaggcagaat aattagatac aggggtcact ctttttgtaa gccaaatacc aaaaaaaaaa
     2821 aattaagaaa aatgataata tcctcataaa tgataatttc aaaaaaaaat atatatatga
     2881 gaaagaaaaa aactgttcta gtaaagtaaa cattaaatct aaagtaattt atactgtgtt
     2941 aaacaataat aatgagatta acttagttta atagacttat ttttttattt ataaaattaa
     3001 ttgggttaat ttgttgtagg tttaatattt tttgttagac tatatttgaa tatatattga
     3061 tgggggtcaa taatattttg ggaaagggtc aatttataaa aattaacaaa acctaaagta
     3121 attttctaaa atccataggg gtcaattgac ccccatgatt atacactccc tccgccactg
     3181 ccggtagggt ttgatgtctt ggattgttaa gttttaaagt tttgtctcat gatctatgta
     3241 agtgcttaag ccggcgctag cataaattga acctttaatc agaatgtagt agttgacctg
     3301 gatggatgac acttttatgc gagatgggtc gagcgacgct aaccattagg ccctcgatcg
     3361 gcagtcggca gatataactt agatacagtg gatagtgaca tatcctctta ataaatatgt
     3421 tttaaatgtt agattaactt atgacctaaa gaaaagaagt tagattaaca ttttttagaa
     3481 attattattt cggataggtt taataagcat aagtcagcga aacttataag gtatttattt
     3541 acaattataa aatcaacagc ttccaaacaa aatctttttt ctttttgact tcttaaaaca
     3601 aaatctcata ctaaccgatt taacattatg tttgtgaata atatggtaac gcgaatgatt
     3661 agcaacataa aaaacaaaga gtcaactaac cgcattcatg aatggaatgg aatgtcacgg
     3721 tcaatcatta gatttggtgt tacggaatac atatttatta aatgtatatg aagaaaaaag
     3781 catggccaat aataatgtaa ataaattggc tcaacatgcg atgtatgtac ataatttaga
     3841 gtggaaaatg aaaagatata tacaatgtgt attaatatag atgtacgcag ttgtataaaa
     3901 ttgatggaca tgatttaggt ttttttttaa aaaattgatg gacatttttc aaatatcaaa
     3961 atgtttaatt tatattttct acatataatt gaaattaaac aaaggagata taagttctac
     4021 ataattttac tttgtatcgt tggaacaaac acgtacagta gtattatttt atatatgtgc
     4081 atgtatgttt tgcgttcctt taaaaatcaa atacatggga agataacatg caacgaacca
     4141 gatgatacag cgcctgatga gaggtcaaaa acgtaacgag tttgacttgt ataatattaa
     4201 tatgtagttg catgtttgat gttgacacag catcgccatc attaattcgt tctctgcagt
     4261 ctaccgaact aattaatctc aaataatcga tgagtacata tatgttaagt ccgtcgaggc
     4321 taatgtataa ttcaataata taaatatgtt cattcgagga tatagttcat attattatat
     4381 atctgtttaa aattattgac tccaatcatc ctataaacta agagggttga caccgcagca
     4441 aagaaaaaaa taaaatatac cataattcat atatttgcac aatctgaatt ataatggaaa
     4501 ttaagtttgc tcaaacaaaa aaaaagggtt ggctccatat acgaaattgt taatttgtaa
     4561 ttttgtagga cttctaatta aaaaaatata tatcaataaa tgattatttt atatatgtca
     4621 attatatctg atcattaatt aattaattgt ttcgaaaata attaattaat tttgaaattt
     4681 aaattaaagc aatattaaat gtaaaaattt taaattatat taaactagaa atttattgtg
     4741 agcatttttg tgtatagatc aagatatgat gaatcttgtg ggattttttt tattctataa
     4801 caggtgttgt ttcatatgct acgcattttt atatatttaa ccaaacataa tgacaagttg
     4861 acaacgacta gtatttcttc ccatttagat gataagatta gtccaaatac tccaaaccgt
     4921 ctgttggatt aagaatgacc ggaatacgat tactccaaaa cgcatatggg tttactggtc
     4981 gagaatatac ctttggatta agatcatgta gagttatttc attacgattt tgatttcttt
     5041 tggttattgt gaatatgttt tttactttct tttttctgtt agagatcatt tgtgaatatg
     5101 ttaagtggta agtgtacaat ttttcatatt ttgaatcgag tatcataaat atcattgtac
     5161 acataccagt ttatttattt cgtcctgata ttaacaagta acaacgtcaa ttttgagatc
     5221 aattatttga aaatatagaa aactgaaagt attaaccaat ttagtttgat tctaattaga
     5281 gtcatcgtat tgtgctagta atcggtgacg ggaaccacat aaaacgacag tttatcaaca
     5341 taaaaaaaaa tcacaaacac atagaagact ctagttttgt tataaacttg tgctaaataa
     5401 ttaacacgat acataatact cttcattatt atatatactt ttcatgtata ataatgcatg
     5461 gacattacac aaatggaatg aatagtacaa ttgtatgtgc atgaatgttt ttctaaaaac
     5521 tgacatccgt ttgtatgcta ttttatcctt gtttgttatt taatagtaga aaacttgcga
     5581 ggaccaaaat aattagcatg caaagaacca gaagaaatcg tgagaactaa aacgtaaaga
     5641 actcttgtga cttgtataat atatagtttg tatgcatgtt tgatgttgac acaacatatc
     5701 tccatcatta attcgtccct tgcagtctct caaacttatt aatctcaaat aatcgatgtt
     5761 tatagattaa gtccctcgag actaataatg taaatccaat gatacaatgt ggttccttcg
     5821 acgacgaatc atattctgat ttctgtatat ctgtaatata aagtacgatt attattatat
     5881 ttgaatacaa tcatacttaa taaaatattg tgtcatatat atttatctcc ataaatgttt
     5941 ctcttgactg aaatgtgaat acccaaacat aaaaacatat acagtaaata ctttgttcaa
     6001 ttcccattaa atttctcttt gaatctaaaa tcagacatag aaatactgtt gtttagagaa
     6061 agattttgaa tatatagaac attaaatcgc cgcagaactt tgttgtttag agaaagattt
     6121 taaatatgat aagaactcaa ctaaagaaaa agaaaaaaaa ttatgacgtc tcaatttaga
     6181 gttttattgg ttcttttgta cagttctgta atgttaggaa tcgttttata cacaaaggta
     6241 acttaaatct ttcaaacgtc tacggtagtg gacggtcatt aaaatcagag ttaagaaaac
     6301 gaacaaacca aatttaatca tatcttatac actcgagacc ttgattatat attactcttt
     6361 tatatattaa cagaactaga aataaaaact atacattatt agaatatcgt aacgtatact
     6421 aatatcccat cgaccagtat ataaacgaca caagtcacag tacattctca tcacatacca
     6481 caaaatatct cataaagatc aaataaacac taaacctgaa agcaaaaaag ggcccatgac
     6541 gcaagaaaga ttcaagaaca accttaacgc cgtcgtgata tcattcttct tcttcttcct
     6601 ctgttccacg tcggatttgc tccttccacg ttcaccgtta gctattctca ccggcgggag
     6661 gtgggatctt ctgcagccga gtgtaggtat atccgcaatg cacatgcagc ttctacacaa
     6721 caacaaagtc gtcatctttg accgtacgga ttatggacct tcgaatgttt ctctcccttc
     6781 tcaaacctgc caaaacgcca ccgtttttga ctgctctgct cattcaatac tatacgacgt
     6841 cgcttcaaat acctttcgcc cacttacact acgttacgac acgtggtgct cttccggttc
     6901 tttaaacgct tccggttctt taatccaaac cggcggttat ggcaacggcg aaagaactgt
     6961 acgggttttc acaccttgcg acggaggagt tggaagcgtt tcgtgtgatt ggattgagaa
     7021 cagagcgtat ctctcctctc gccggtggta ttccaccaat cagattcttc ccgatggtcg
     7081 gattatcatc gtcggcggaa gaagagcttt caactacgag ttttacccca aggatccggg
     7141 agaatccgtt ttcaatctca gattcttggc ggagacgaga gatccgaatg aagaaaacaa
     7201 tctttatccg tttcttcatc ttctccccga cggtaatctc ttcatcttcg ccaatcggag
     7261 atcgattttg ttcgatttcg tcaatcaccg aatcatcaag gagtttccac agattcccgg
     7321 cggcgataaa cggaattatc ccagcactgg ctcctccgtg ctcctccctc tctttctcac
     7381 cggagacatt aacagaacga agataactgc tgaggttatg gtatgcggcg gtgctccgcc
     7441 gggagcgttc ttcaaagcgg cgaggacgat tcctaaaatc ttcgtcgcgg gatcacgcac
     7501 gtgtgggaga ttgaaagtga ccgacccgga tccgaaatgg gtcatggagc aaatgccatc
     7561 gccacgtgtc atgtctgata tgttactgtt accaaacggt gacgttttga tcattaacgg
     7621 tgctgctaac ggaaccgctg gttgggaaga cgcgacgaac gctgttctca acccgatttt
     7681 atacttaccc gaggagcctg accagacccg gagattcgaa attctcaccc cgactcgaat
     7741 ccctagaatg taccattcgg cgtctcttct tctgtcagac ggcagagtct tagtcggagg
     7801 aagcaatcct catcgaaatt acaatttcac ggcgaggccg tacccaacgg agctcagcct
     7861 cgaggcttat ctcccgcgtt acctggatcc acagtacgca cgtgtgagac cgacgatcat
     7921 cacggtggag ctcgccggga atatgttgta cggtcaggct tttgccgtaa cgttcgcgat
     7981 tccggcattc ggaatgttcg acggaggggt atcggtgagg ctcgtggcgc cgtcgttcag
     8041 cactcactca acggcgatga accagaggtt gttggttttg cgcgtcaggc gcgtgagtca
     8101 gttatctgtt ttcgcttata aagctgacgt ggacgggcct actaactcct acgtggcacc
     8161 acctgggtat tatatgatgt tcgtggttca ccgtggaatt ccgagtgtag ctgtttgggt
     8221 taagattgga agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcc
     8281 tggaggtgga ggtggagctg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     8341 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     8401 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     8461 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta
     8521 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     8581 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     8641 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     8701 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     8761 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     8821 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     8881 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     8941 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     9001 cgagctgtac aaggatcctg ctggtgctgc tgcgcctcta gcataagcgg ccgcgatcgt
     9061 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     9121 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     9181 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     9241 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     9301 ctagatcgag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     9361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9421 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg aaggccttga
     9721 caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctaattccgg
     9781 ggatcggaaa tccagaagcc cgagaggttg ccgcctttcg ggctttttct ttttcaaaaa
     9841 aaaaaattta taaaacgatc tgttgcggcc ggccgccggg ttgtgggcaa aggcgctcga
     9901 cggtgggcaa ccgcttgcgg ttgtccacgg gcggagccgg tgcgcgtagc gcattgtcca
     9961 caagccaagg gcgaccaata attgatatat atattcataa ttgaaaagct aattgaacat
    10021 actacttgct gtaactactt gccggagcga ggggtgtttg caagctgttg atctgaaagg
    10081 gctattagcg ttctcacgtg cctttttgat tagcgatttc acgtgacctt attagcgatt
    10141 tcacgtactc cgattagcga tttcacgtac cctgattagc gatttcacgt ggatagtttt
    10201 tggagcgggc cggaaagccc cgtgaatcaa ggctttgcgg ggcattagcg gtttcacgtg
    10261 gataactacc ctctatccac aggcttccgg ggataaaaaa gcccgctcga cggcgggctg
    10321 ttggatgggg atcgcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt
    10381 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga
    10441 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt
    10501 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
    10561 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
    10621 gagccatatt caacgggaaa cgtcttgctc aaggccgcga ttaaattcca acatggatgc
    10681 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
    10741 ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
    10801 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
    10861 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
    10921 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
    10981 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
    11041 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
    11101 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
    11161 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
    11221 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
    11281 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
    11341 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
    11401 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaacc
    11461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11521 atcaaaggat cttc
//
