LOCUS       sequence-345734-        4994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 
            (At5g63800) signal peptide (84bp) fused in-frame to the coding 
            sequence of the RG-I degrading enzyme RglA (AN7135.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW
  TITLE     Pectin Modification in Seed Coat Mucilage by In Vivo Expression of 
            Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes.
  JOURNAL   Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 
            10.1093/pcp/pcab077.
  PUBMED    34059917
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077."
COMMENT     Sequence Label: pDONR207-MUM2sp-RglA
FEATURES             Location/Qualifiers
     source          1..4994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2055..2154)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2266..2285)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2344..2363)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2937..3470)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3659..3664,3665..3681,3682..3687))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4344..4932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4833..4852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      421 aaagcaggct atggagatgg gtcgtctggt cttcgggttg tgtttgattc ttattgtggg
      481 tacgtttctt gaattctccg gcggtgctac ggcggcacta gtggcctttg gcattaccga
      541 caacggcagt tcctacacga tcgacgccaa ctctcccaac ccgctgaaat tcactgtcaa
      601 taagtccagc tgcgacatca catcaattgt ttactatgga tccgagtttc agtattccgg
      661 aaaagggagt catattggct ccggtctcgg ctcggccaca gtctctgcga ctcagagcgg
      721 cgactacatt aaggttactt gcgacacatc aagcttgacg cactatttcg ttgtgcacaa
      781 tggtgacccc atcatccata tggcgaccta tattactgca gagcctgata ttggtgagct
      841 gcggtttatt gctcgactta actcaaacct cctgccgaat gaagagccat ttggggatgt
      901 atctacgaca tccggcgggt cagctatcga gggctccgat gtattcctgg tcaacggcga
      961 gacccgtagc aaattctact ccagcgaacg gtttatcgat gaccacaggc actgcatttc
     1021 gggaagcgca caccgtgtct gcatgatctt gaaccaatac gagtcttcct ctggtggtcc
     1081 tttcttccgc gacatcaact ccaataacgg cggtgattac aatgccctct attggtacat
     1141 gaactccggc cacgtccaaa ccgaatcatt ccgtacgggc ctccacggtc cctactcgat
     1201 gtacttcagt cgaagcggaa cccccagcac caacatcgac acatccttct tcgccagcct
     1261 tggtattaag ggttatgttg ctgccaacgg acgtggaact gtcaccggca aagcttccgg
     1321 cgcagactcg agcatggact gggtggttca ctggtacaac aatgatgccc agtactggac
     1381 ctacacagcg tccgacggca gtttcacctc tcctgccatg aaacccggca cgtacacgat
     1441 gaaatactat caaggcgaat tcccagtggc ggaaaccacc gtgactgtat cggccggatc
     1501 atcgaccacg aaaaacatct ccggctcggt caaaacaggc acgacgatct tcaaaattgg
     1561 agaatgggat ggccaaccaa ctggcttccg caacgcagat aaacaactcc gcatgcaccc
     1621 ctccgactcc cggatggact cctggtcttc cacctacaca gttggctctt cttcgctctc
     1681 tgacttcccc atggctgtct tcaagagcgt caataacccc gtgacgatca agttcaccgc
     1741 tacttctgca cagacaggtg ctgcaactct ccgcatcggc acaaccctct ccttcgctgg
     1801 tggtcggccg caggcaacga tcaacagcta taccggcccg gctccctcag cgcctactaa
     1861 tctcaactcc cgaggtgtca cgcgaggtgc ttaccgtggg cttggggagg tgtatgatgt
     1921 tagcgtgccc gctggaacaa ttgttacggg cgagaatacg atcactataa gcgttatttc
     1981 cggaagctca ggtgacgcct tcttgagccc taacgtggtt tttgattgca ttgaactttt
     2041 ccatggacgt ccttacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2101 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2161 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2221 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2281 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     2341 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     2401 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     2461 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     2521 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     2581 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     2641 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2701 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2761 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2821 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2881 ttatttttga cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag
     2941 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     3001 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     3061 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     3121 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     3181 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     3241 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     3301 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     3361 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     3421 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     3481 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3541 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     3601 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     3661 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     3721 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     3781 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
     3841 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
     3901 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     3961 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat
     4021 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     4081 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     4141 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     4201 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     4261 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4981 tttgctcaca tgtt
//
