LOCUS       sequence-345736-        5388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 
            (At5g63800) signal peptide (84bp) fused in-frame to the coding 
            sequence of the RG-I degrading enzyme RglB (AN6395.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW
  TITLE     Pectin Modification in Seed Coat Mucilage by In Vivo Expression of 
            Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes.
  JOURNAL   Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 
            10.1093/pcp/pcab077.
  PUBMED    34059917
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077."
COMMENT     Sequence Label: pDONR207-MUM2sp-RglB
FEATURES             Location/Qualifiers
     source          1..5388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2460..2559)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2671..2690)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2749..2768)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3342..3875)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4064..4069,4070..4086,4087..4092))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4749..5337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5238..5257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      421 aaagcaggct atggagatgg gtcgtctggt cttcgggttg tgtttgattc ttattgtggg
      481 tacgtttctt gaattctccg gcggtgctac ggcggcacta gtggccctaa ctgcaacaga
      541 gaatgacacc tccattgtcc ttgagaacga ccggctcaga gccaccttcg acaaaggcag
      601 ggggagtatc atcgacctct accttgacgg tcaagatttc ctaggtccgc agtctggatc
      661 gaccgggatc ggcccctatc tcgactgcta ctgcaccccc tccggtttct acaccgctgg
      721 ttcaacaaat cctgtaacag agctggtgca aggcacagac tcgaccggca caaaatatgc
      781 aggcatcatc ctgaacgata cgtatacccc gaccggccaa gagttccagc agtactggtt
      841 cctccgcgat ggcgagaccg gtttccatat gttcagtcga ttggcgtact acaacgagac
      901 caccccgttc ctgcgcaatc tacaagagct ccgcacgctc tttcgcccga atacggatct
      961 atggacacat ctcacctcga gtgacctgca gactgcgccc ttgcccagcg acgaggctat
     1021 tgcggagcag atcgttgtcc aggacgcgac ctggaggctt aataacacgc cggacgatgc
     1081 atactaccag cagttttcag agtactttac caagtacacc ttctcgaacc actggcgaga
     1141 caatgatgtc cacggactct acgcggacgg gtctacctcg gatgggacta catacggcgc
     1201 atggctggtg atgaatacaa aggataccta ctacggaggc ccgctgcact ctgacctcac
     1261 cgtcgacggt ataatctaca actacatcgt ttccaaccac cacggcgaag gcactccgaa
     1321 cataaccaac ggctttgacc gcaccttcgg accgcagttc tacctcttca acggcggagg
     1381 atcctcctca ctagaggagc tcagagacga ggctcgctct ctagcgagcc caagctggaa
     1441 cgccgacttc tacgactcga tcgccaaaca cgtcattggt tacgtccctt cgtcgcagcg
     1501 cggcagcgta aaaggcacga tcaagctgcc caagaacgcg aaaagtccca tcgccgtcct
     1561 caccgtcgac ggccactact tccaggataa ctctgccgtt ccctcgtccc accaatactg
     1621 ggccgacatc gacaagaatg gccgcttcag catcgaccgc gtcgttgccg gcaaataccg
     1681 actaaccgtc tatgcagacg gcattttcgg cgacttcaca cgcgacggga tcgttgtcaa
     1741 agcacgcaaa tcaacatcca tcaaggagac atggaagccc gaatctgctg gcacagagat
     1801 ctggcgtctc ggtacccccg acaaatcctc cggcgagttc cgccacggcg cagcgcgcga
     1861 tcctacccat ccccgccacc cgccagagta cctgatctac tggggtgcct acgactggca
     1921 atctgacttc cccggcggga ttgactatat gatcggtgag agcgatccag caacagactt
     1981 caatacagtc cactgggctg tcttcggacc cacgcccgac aaccccgtag cggaatcgaa
     2041 caccacccac gactggagaa tccgatttga tctaagcgcg aaacagctac acgcacgtaa
     2101 gacggccaca ctaacgatcc agcttgcggg tgcaaaggct gcgagcggaa acacggacgt
     2161 gtataacgcg tccgagccgt acgcgaatct cccgctgcgg agctacatca atgagcaaga
     2221 ggagcctttg actatggtta ttggctatga tcaatcgagt agctgtattg tgcggtcggc
     2281 agttagttgc taccaggtgc gtgagaaatg ggagttccca gctagttggt taaaggaggg
     2341 aagtaattta ctgaggctga gcttgccgac taatgggacg aattacgaga gtgcggtgtt
     2401 gccgacgagc gtgtacgtgc agtatgatgc tttgagattg gagttgaatg gacgtcctta
     2461 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2521 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     2581 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2641 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2701 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2761 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2821 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2881 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2941 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     3001 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3061 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3121 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3181 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3241 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3301 ggaaattagg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     3361 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     3421 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     3481 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3541 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3601 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3661 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3721 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3781 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3841 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3901 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3961 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     4021 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     4081 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     4141 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     4201 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     4261 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     4321 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     4381 gttcgcccag gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt
     4441 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     4501 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     4561 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     4621 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     4681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4861 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5341 gccagcaacg cggccttttt acggttcctg gccttttgct cacatgtt
//
