LOCUS       sequence-345737-        4550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 
            (At5g63800) signal peptide (84bp) fused in-frame to the coding 
            sequence of the HG degrading enzyme PelA (AN2331.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW
  TITLE     Pectin Modification in Seed Coat Mucilage by In Vivo Expression of 
            Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes.
  JOURNAL   Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 
            10.1093/pcp/pcab077.
  PUBMED    34059917
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077."
COMMENT     Sequence Label: pDONR207-MUM2sp-PelA
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1611..1710)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1822..1841)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1900..1919)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2493..3026)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3215..3220,3221..3237,3238..3243))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3900..4488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4389..4408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      421 aaagcaggct atggagatgg gtcgtctggt cttcgggttg tgtttgattc ttattgtggg
      481 tacgtttctt gaattctccg gcggtgctac ggcggcacta gtggtctccg tcagcggctc
      541 ggccgagggt ttcgccaagg gtgtgaccgg cggtggtagt gccacagccg tctacccaga
      601 caccatcgac gagctggtct cgtacctggg agacgacgag gcccgcgtca ttgtcctctc
      661 taagaccttc gacttcaccg acagtgaagg cacgaccacc gaaaccggtt gcgcaccttg
      721 gggcaccgac gcggcgtgcc aggtcgcgat taaccagaat gactggtgca caaactacca
      781 gcctgacgct ccctccgtct cggtgaccta cgacaatgcc ggaaccctcg gtatcaccgt
      841 taactcggac aaaacgctta tcggcgaggg ctccgccggt gtgatcaagg gcaagggtct
      901 gcgccttgtt agcggaacca gcaatgtcat catccaaaac atcgccatta ctgatatcaa
      961 ccccaagtac gtctggggag gtgacgccat cactctgaac gacgtggata tggtctggat
     1021 cgaccacgtt actaccgctc gcattgctcg tcagcacatc gtcctcggca ccgaagccga
     1081 caaccgcgtg accatctcca actcgttcat caacggtgaa tccgactact cggccacttg
     1141 cgatggttac cactactggg gtatctacct cgacggctcc agcgacatgg tcaccatgaa
     1201 gggcaactac atctaccaca ccagcggtcg tagtcccaag gtccagggta acactctgct
     1261 gcacgctgtc aacaactact ggcacgacaa ctctgatcac gccttcgaga tcggtgaggg
     1321 tgcctacgtg ctcgctgaag gaaacgtttt ccagaacatc cccaccgtgg ccgaggaccc
     1381 cattgagggt gagctcttcg cttctccctc cgaatctgcc aacgaggttt gctcgactta
     1441 ccttggccgt gtttgcgagc tcaacgggtt cggcagctct ggcaccttca accaggccga
     1501 caccgatttc ctcagcaagt tcgagggcaa gaacatcgca tccgccgact cctacagcag
     1561 cgtcgcttct agtgtcgcct cttctgccgg taacaccctt ggacgtcctt acccagcttt
     1621 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     1681 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
     1741 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1801 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1861 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1921 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1981 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2041 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2101 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     2161 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2221 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2281 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2341 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2401 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattag
     2461 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     2521 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     2581 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
     2641 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     2701 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
     2761 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
     2821 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
     2881 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
     2941 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
     3001 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     3061 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
     3121 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     3181 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     3241 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     3301 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     3361 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     3421 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     3481 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     3541 ggactctagc tatagttcta gtggttggct actaataggt tgtattgatg ttggacgagt
     3601 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3661 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3721 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3781 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3841 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3901 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3961 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4021 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4081 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4141 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4201 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4261 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4321 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4381 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4441 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4501 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
