LOCUS       sequence-345739-        8642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genomic fragment containing the upstream region and coding region of
            CER11/CPL2 cloned into pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L
  TITLE     ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory 
            Trafficking.
  JOURNAL   Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. 
            Epub 2019 Sep 4.
  PUBMED    31484679
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00722"; journalName: "Plant Physiol"; date: 
            "2019-11"; volume: "181"; issue: "3"; pages: "901-915"
COMMENT     Sequence Label: CPL2g/pDONR221
FEATURES             Location/Qualifiers
     source          1..8642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3054..3065
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6767..6866)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6883..6902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6884..6902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6907..6923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6907..6923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7025..7044)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7036..7845
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7103..7122)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7992..8580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8481..8500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttt aagctcggaa tcaagtgtca acattttgtg cttagacggt agcgacgata
      721 atctcagcga catgagttgc tcttaagagt tacaacttac aacaagtaac tataacttta
      781 agcgacataa gttgagatac atatttaaat actctcatga aggttctcaa ctattagaat
      841 ggccatgaat tttgatttac atgttaattt tggaccgaag aggcaattgt tttccgactt
      901 gcaataaaca tctgtctctt agaatcttga taataatagg gttaagttgc aaagtagtca
      961 cttatttaaa cttaattatg gaattgggca agttactatt tataattgga tttttagtca
     1021 ctgacctttg accctattac agacaaaatc aaatataccc cataccaact ctattattta
     1081 caaatatgcc actaatcttc ttcttctcta atgtcgttga aagcaattgg gatttgtgct
     1141 tttagattca acatttctct ctcctcatac tctcattctt tgtatttctt tatatatata
     1201 tatatttatt tattttttaa taacctcttc aatctaataa ttgaaatgct tatcaatgta
     1261 aatttatttt ctaaattaat tcagatacat aaaaatctat atttttttct ctataatttg
     1321 atatgcattt tgttaataat tttaaatata ttatataaac atttttatta gtagtcagta
     1381 cagttaatta tgtgtacaga tttttattta tacaacttat ttaataagta caaattgaaa
     1441 tcgtccagcc ataattggga aaatagtcat tttactattc acaatttgca aagtagtcac
     1501 ttttaactgt ttgattcgta cacttagatc tgtacaacac tttttagtgt acagatgtaa
     1561 gtgtatgttg tgtatattta tatatatata tatacactaa aaatattgta caggtctaag
     1621 tgtacgaatc aacagtgaaa agtgacttct gataattgta tactccatac aattatatac
     1681 tctgtacagc taatgcaaca tacaaaataa agtctgtaca cataattaag tgtaccgact
     1741 actgataaaa tgtctgtaat atattaaaaa atattaacaa aatgcatatc aaattataga
     1801 gaaaaataca tagattttta tgtatatgaa ttagtttaga aaataaattt atattgataa
     1861 gtatttcaat tattaaattg gaggggttat taaaaagaaa taaaaaaaaa aaagaatatt
     1921 tcacttttat ctttcttctt tgtcaaaagg atagttcggt aatttcaaaa cacatcactt
     1981 accccacccc aaacctctat ttcacgctcg tctattacac gcccaaagtg agttacctgt
     2041 tttgccaatt ataaatagta acttgcccaa ctccataatt atgttttcaa aagtgactaa
     2101 tttgccaaca cacccataat aatatgatga tacagattgt ataaaattga tttgtccctt
     2161 tcttactcaa acgtcacaag actcgcaact tgatgtcttc tctttttttg ccaaccaact
     2221 ttatgttctt atttgatttt tgtgatttta ccatatatat atcctcttcc gttttttttt
     2281 cacaattttt atttacttct aaagtaagtt attgaatacg tttccacatt ttacaatata
     2341 tagttgtaaa cattaactat ccatttgttc gaagtcaacc aaaattatcg acctaatccg
     2401 atttcgtcat acacacaaaa aataattctc gaatatttta ttttgttagg aacggtaatt
     2461 gacggctgac aagcgatgtt ggtgaaaaca ggataatgca tctgacgcca cgtgtaaaga
     2521 agtctcaggt tttctcgtaa tttacattaa aatcgatgtc gtaacaataa aacgcttctc
     2581 tcgcctataa atatatatct gagcctactt tggttctcat gtttaaggag acccagaaaa
     2641 accctcattt gcggagaaaa atcacagaga aaagtagcga agtttgagag aaattgaatc
     2701 tgcagattct ctcacgcatt ttcccagaaa ctgtttatct tcactacctg attcgtcctt
     2761 cagaagtgtg aattgaacga attcacgatg aatcgtttgg gtcataaatc cgtggtttat
     2821 cacggagatt tacgtttggg ggaacttgat gtgaaccatg tatcttcaag tcatgaattt
     2881 cgattcccga acgatgagat tcgaatccac catctatctc cggctggtga gagatgtcct
     2941 ccgctcgcga ttcttcagac gattgcttct tttgccgttc gatgcaagct tgagtcatcg
     3001 gctccggtga aatctcaaga gttaatgcat ctacacgcag tctgtttcca tgaattgaag
     3061 gtaggtttga gatttccgaa atgacattta gctgattttg gatgttttcc aattgttagg
     3121 agtgattcac tagtttgatt ttgtgaatac agactgcagt ggttatgctc ggagatgagg
     3181 agattcattt agtggcgatg ccgagtaagg agaagaagtt cccgtgtttt tggtgttttt
     3241 cggttccttc aggtctttat gattcgtgct taaggatgct taatactcgg tgtttgtcta
     3301 ttgtgtttga tttggatgag actttgattg ttgcaaatac tatgaagtca tttgaggata
     3361 gaatcgaggc tcttaaatct tggatttcac gtgagatgga tcctgtgagg attaatggga
     3421 tgtcagcaga acttaaaagg tatatggatg ataggatgct gctgaagcag tatattgata
     3481 atgactatgc ttttgataac ggagttttgt taaaggctca acctgaggaa gtccgcccaa
     3541 cttctgatgg acaagagaaa gtttgtagac ccgtgattag attgccggag aagaatactg
     3601 tgcttactcg gattaagcca gaggttagaa gattcatctc tgttgtggct ggatatttat
     3661 tcatttcatc ttatttgatg ctttggattt accttctctg gcatgtgata ggataaggtc
     3721 gcttaagtat atttgagtga tttagcttta atattgttgg aactgaaacc aagttattgc
     3781 tgatgcagat ccgtgacact agtgtgcttg taaaactgag accagcatgg gaggagttaa
     3841 gaagttattt gacagccaaa actaggaaac gttttgaagt ttacgtttgt accatggctg
     3901 aaagggatta tgctctggaa atgtggagac tgcttgatcc ggaagcacac ttgataagtc
     3961 tcaaggagct tcgagatcgt atcgtctgcg taaaaccagg tcaaaaccaa ctctggcttc
     4021 tttgataccc ttgatcttgg ttttggtaca tagccaccat gctccattag tgtagctttc
     4081 attactcttt ttgtggtgaa ttgtttaata taatgtgcct cttctccctc cctctatcgt
     4141 gcctgattga tttgccgacc tctcagtttt tagtattaag agatgaacct gatcattttt
     4201 tacccaacta tctgtctctg tctctctctg aaactcgaac taacaatttt tttatgtctt
     4261 gctctttgca tatcagacgc caagaaatcg ttgttaagtg tcttcaatgg tggcatttgc
     4321 cacccgaaaa tggcaatggt cattgatgac cgtatgaagg tttgggagga caaggatcaa
     4381 ccacgggttc atgttgtttc tgcatatctt ccttattatg ctccacaagc agaggcatgt
     4441 ggttgctctt gcatttagtt gtgaattaaa ttctgtatca cttaattttc caagtgggta
     4501 tctaaccatt ttcgtatgcc catttatcag acagcccttg tcgttccaca tctctgcgta
     4561 gcaagaaatg ttgcatgcaa tgtcagaggt tactttttca agtaagttgg aaaaaaaaat
     4621 tatgtttttt ttttattttt atgtaatgaa ttcatgctgg ttgttcttac tgtttctaga
     4681 cttttatggc agggaatttg atgagagttt aatgagtagt atatctttgg tttactatga
     4741 agatgatgtg gaaaatttac ctccatcacc ggatgtgagc aactacgtcg ttatagaggt
     4801 aactgtagtt agaactttat cattgttaac atattgctaa tttgccccaa ctacaatatg
     4861 taatctagct atatctgtct gtaggatcct ggctttgctt caaatggaaa cataaatgct
     4921 cctccaatta acgaaggaat gtgtgggggt gaagtggaac gaagactaaa ccaagcggtt
     4981 agtaaaatat atacaaaatt tgggattaat taactaaaat atgttttggt gcatagcttt
     5041 tgtgtcagtt gtaatttgat ttctgttgac ttttcaattt ctgggatgag aaggctgcag
     5101 ctgatcattc tactcttcct gcaacaagta acgctgagca gaaacctgaa acccctaagc
     5161 cgcagatagc agttatacca aataatgcaa gcacggcaac tgcagcagca ctgttacctt
     5221 ctcatagtac aaacttttga cttaatataa ctttaaattt tgaactgtac agttattttg
     5281 cacgtatatg tttgtctctg acgttctcta tctcttgtct tcaatcacaa tctagaacct
     5341 agtttgcttg gagctcctag gcgggatggc ttcacttttt ctgatggtgg aagacccctt
     5401 atgatgaggc ctggtgtaga tataaggaac caaaacttta atcaaccccc cattctggct
     5461 aaaattccta tgcagccacc ttcctcgtct atgcattctc cgggtggttg gttggtagat
     5521 gatgagaata gaccttcttt tcctggtcgg ccttcaggac tttatccaag tcaatttcct
     5581 catggaacac caggttctgc tcctgttggt ccatttgcac atccatccca cttgaggagt
     5641 gaagaggtta gttttgcgtt ctaagtgtct ctcttttgac atcaaagtat gttgtctacg
     5701 gtctgatagt tttcttgcag gtagcaatgg atgatgacct gaaaaggcaa aatccttcac
     5761 gtcaaacaac aggtatgttc tagtttcaac actaatgcaa tcaagctgat attaaagatg
     5821 attagtctat ccctgaattt cttgtttcct ccgtgaagct tgttttcagt gtaccgctct
     5881 aaagctacat tagatattga cttaggatta gagaattgtt ggtttcccta tctgatgtat
     5941 tgtctttcta ctgaatctgt aacagctact tgaataataa ggtgcattaa taccttttta
     6001 acattggcac ttgtttaaac tatttgcaga aggtggaata tcccagaatc atttggtgtc
     6061 aaatggtaga gaacatcata cagatggtgg aaagagtaat ggcgggcaat ctcatttatt
     6121 tgtgagtgca ctgcaagaaa ttggaagaag atgtggttca aaggtgcata ttcactaacc
     6181 acgcaagcca ttgcttcttc tcttttaatg gattctgatt aattgatgta cgattttgca
     6241 ggtggagttt aggactgtga taagtaccaa caaagagtta cagttttcag ttgaggtcag
     6301 ctggatcagt ttattgaagc atctttgtct attgttagct ttacttatat ttgtttttaa
     6361 tcgttgttgt taattttaca aaggtgttgt tcacgggtga gaagataggc attgggatgg
     6421 caaaaactaa gaaagatgca catcaacaag cggctgagaa tgctcttcga agtttggctg
     6481 gtaaagtttc ttctagggga attaataact gtggatctat ttcaccactt gattaagtct
     6541 gcagattgtt cttagtcttt tgtgtagtga gcaaatttgg ttacagattg gtaaatcttc
     6601 tttgtgttgc tttgtgcaga aaagtatgtt gcacatgtgg cacctctagc tagagaaaca
     6661 gagaaaggtc cggaaaatga taatggattt ttgtgggaaa gcagtgaaga tgtatcaaac
     6721 aaaggacttg aagaggaagc gcctaaagaa aacatttcag agttgcaccc agctttcttg
     6781 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     6841 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     6901 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     6961 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     7021 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     7081 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     7141 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     7201 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     7261 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     7321 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     7381 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     7441 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     7501 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     7561 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     7621 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     7681 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     7741 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     7801 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     7861 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     7921 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     7981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8641 tt
//
