LOCUS       sequence-34574-e         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-p53shRNA
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..219
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     140..159
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     polyA_signal    324..457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(348..367)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     402..421
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttctaacgct gacgctcatc aacccgctcc aaggaatcgc gggcccagtg tcactaggcg
       61 ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca ggggagtggc
      121 gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc
      181 tttggatttg ggaatcttat aagttctgta tgagaccaca aatccccgac tccagtggta
      241 atctacttca agagagtaga ttaccactgg antctttttg gaaaagctta tcgataccgt
      301 cnacctcgag gccggcaagg ncggatccag acatgataag atacattgat gagtttggac
      361 aaaccacaac tanaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      421 ctttatttgt aaccattata agctgcctaa acaagttaac aacaacaatt gcattcattt
      481 tatgtttcaa gttcaggggg aggtgtggga ggtttttt
//
