LOCUS       sequence-345740-        5711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genomic fragment containing the upstream region and coding region of
            DET3/VHA-C cloned into pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L
  TITLE     ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory 
            Trafficking.
  JOURNAL   Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. 
            Epub 2019 Sep 4.
  PUBMED    31484679
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00722"; journalName: "Plant Physiol"; date: 
            "2019-11"; volume: "181"; issue: "3"; pages: "901-915"
COMMENT     Sequence Label: VHACg/pDONR221
FEATURES             Location/Qualifiers
     source          1..5711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3836..3935)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3952..3971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3953..3971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3976..3992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3976..3992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4094..4113)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4105..4914
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4172..4191)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5061..5649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5550..5569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttt agatgaatca atcttcttac ttcagagact cgcgattttg gtttgaggac
      721 gacgattcag agttagggct ttgaagctga atcaaaacac tcgagccaat ttagtttgta
      781 accctagaag tttttgtagg gttttaccgt ctctgttttt tattatttgg atctctgaac
      841 acatttggcc cagtattagc ccatttacct atcttctcaa aatatttggt tcttaacaaa
      901 ttcaagccta accattagta caattttaat tttgatctgt acattttcaa aaaccggact
      961 cgaaccaaga tcaaattcaa caattgtggc aattttaatg gtcgagtctc cagtgtgtat
     1021 gtggcagtag gagaacgtgc cgggggatat taaaccggct caatgctaca gtcgtaccgg
     1081 tttgtttttt tactcgttgg tatataatta aaaccggtta tccaatttga caggcagaga
     1141 gaagaagatt tgattgaagt acagatccat cggagaagaa gacagagaga ttgaagatca
     1201 atgacttcga gatattgggt ggtgtctctt ccagtgaaag actctgcttc ttcgttatgg
     1261 aatcgattac aagagcagat ctctaagcat tcctttgata ctccagttta tcgggtactc
     1321 tgatttttgc tcgatttggt cttattgcaa aatttagcac agatcttata ctatctcttg
     1381 ctactttcgt gtctacgatt ctaattacga tctaattccg tgagttttgg tttcaatttc
     1441 gcagtttaac attcctaatc ttcgtgttgg aaccttagat tctctactcg ctctcggcga
     1501 tgatctgctt aaggtaaaat tcatttgatc actgagtgga ttttatgttt ttgattttaa
     1561 aattcctctc ttgattgaga aatttttgag atttgtagtc gaacagcttt gtggaaggag
     1621 tttcgcaaaa gatcaggaga cagatcgaag aattggagag gatttctggt gttgagagca
     1681 acgctcttac agttgatgga gttcctgttg attcttatct caccaggtat ttgtgatttt
     1741 ccgtctcaga aggagttaat tgctaatttg gtggtgatcg aattgttaaa gaacgaaact
     1801 tttgttgatt ggtggactca aggtttgtgt gggatgaagc taagtaccca acaatgtcac
     1861 ctctgaagga ggttgtggac aatattcagt ctcaggttgc aaaaattgag gatgatctca
     1921 aggtattgta gcttaaacct tttgttttat tgtaaaaaaa aaggaagctt cacctttaag
     1981 cagaaagatt agctgcatga gaccgatggt ataaaatttg caattcgagt ttcttacgat
     2041 gatcaaatga attgctttgg ttcaaatggt ggtgttaacg actctgattg cacattttat
     2101 tcaggttcgt gttgctgaat ataacaatat ccgtggtcaa ctcaatgcca ttaaccgaaa
     2161 gcaaagtgga aggtaattct tttcttctca tatttatact ataagagcac actagaaact
     2221 gtgggcggtt tagcaacaac aaattttgaa gtttgttttc tcatctatct gatttttgct
     2281 tgaagcatga tttaccacgt gaacgtttta gaataataca aagttatttt ccagaatatt
     2341 gctgttggtc tttatcgata tctcagcttt gtttagtatc tttgtggcat tgtggctgat
     2401 cgtcctcctt tttgtctcag cttagctgtt cgtgacctct caaacttggt taagccagaa
     2461 gatattgtcg agtccgaaca tctcgtaact ctccttgcag ttgttccgaa gtattcccaa
     2521 aaagactggc tagcatgtta tgagactttg accgattatg tggtaaattt gttgacttgc
     2581 taggtttatt gaatagacca gtggtaacac tactggtttg acgtaccgtg attttaatca
     2641 atttatccta acttgtttca ggttcctagg tcctcgaaga aattgtttga ggataatgag
     2701 tatgcccttt acaccgtcac tctctttact cgtgtcgcag ataatttcag gattgctgct
     2761 cgtgagaaag gattccaagt gagtattctg ttcaagttcc tgatattacc tctagagtta
     2821 cgtagaagtt aaaaatgatt acatgctagt aggagtgaaa tttcctacga actgattgta
     2881 ttcatggtgc aagttcagaa atttggttta gattcaaaga tttgttcatc atatattgta
     2941 caccatcctc ttctaggtcc gtgattttga acaaagtgtt gaagcacaag agactcgtaa
     3001 acaagagcta gcaaagttgg ttcaggatca ggaaagtttg agaagctctc ttttgcagtg
     3061 gtgctacacc agttatggag aggtaggtat ctacacattg ctctgaatag atattctctt
     3121 gtaatacaat acctgaatct gttttgatct tcggtctaca tctgtaggtt ttcagctcct
     3181 ggatgcattt ctgtgctgtg cgtacattcg ctgagagcat tatgagatac ggtttacctc
     3241 cggcgttctt ggtaaattag gactccaagc tatgctattt tagagaattc ctctacccta
     3301 ctatctccct taccaaatta ctttctcttg tctttccagg catgtgtctt atctccggct
     3361 gtgaaaagtg aaaagaaagt acgctccatt cttgaacgct tgtgtgattc taccaacagg
     3421 tatgttaaaa ctctctacct atctatcgct ctaggtgtag gatacatgga agctgtttaa
     3481 catcaacctt catattgatc tggcgaagaa aaatgtactg atagttatga ccttaataac
     3541 gaaagaaatt gcagaagttt gaggagaaag tcaggaacca agtaaaacca aacttgtatt
     3601 agcatgcaat gcgttcttga ggctaaatct attaatcaga actaagtcgc tttagttaga
     3661 ttcatctatt gatgtagtgt agttcgatga gatttcaaat tgagcgtttt gggtctcatt
     3721 gtatgaaaac aatgcagttt atactggaaa agcgaggagg atgcaggagc catggctggt
     3781 ttagctggtg actcagagac acatccttat gtctccttca ctatcaacct tgctcaccca
     3841 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     3901 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     3961 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     4021 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4081 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     4141 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     4201 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     4261 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     4321 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     4381 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     4441 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     4501 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     4561 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     4621 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     4681 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     4741 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     4801 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     4861 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     4921 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     4981 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     5041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5701 gctcacatgt t
//
