LOCUS       sequence-345743-       15745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 15745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL2:GUS
FEATURES             Location/Qualifiers
     source          1..15745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2391..2396,2397..2414,2415..2421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2434..2456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2453..2469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2453..2469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4379..6190
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      6261..6513
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6522..6539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6522..6538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6531..6553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6678..6697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6888..6909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7156..7180
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(8776..9924)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10223..11017)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11209..11976
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      12599..13310
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(13437..13808)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            13841..13950
                     /note="incP origin of transfer"
     primer_bind     14173..14192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14426..14443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(14631..15281)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gctctgaggg aggcggttcc
     1921 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg
     1981 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     2041 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     2101 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     2161 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct
     2221 caatcggttg aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2461 agctatgacc atgattacgc caagcttgca tgcctgcagg tcgacatgta aaatatgatg
     2521 acgcaaacac atatatatag caagatttgt tccgacaaaa cttaagaaaa caaaaaaaaa
     2581 aaaggcaaag gaaaaagaaa cacaaatttg atttttggga attcgaattt tcaaccgcca
     2641 acattttaac aaaaatatgg atcgtgtcac atcaacaaca ctaacacaaa ttattagact
     2701 acttcttaac cagctattta attgtaattg tttttttatt tgtttttact gtaataaata
     2761 aacaactaac atgtaaattt ttggtaaatg ataacagtaa atgtctagat cgtgattagt
     2821 aaattgacat ttccaaattt tagtgacgga tttgtgttta gattcgatac tgtatataga
     2881 gtatggagaa gtatgaacac ctcccaaatt acttgcataa gaaatgatta attggaacag
     2941 ctttccacta ccacattagc ttgcatatca ttccgaagca cttgcatttg ctcaaaccaa
     3001 aagaagatgg agctaaacaa aaataaacta gcttgaaaaa aagataggtg taacatgaga
     3061 taagaaataa tggcatttct atcaaagtgc taggaaacaa agttaagttt attatagcac
     3121 ccgaagatta atccttatta tgcaaattaa gtttctagaa gtgggtcatc tcacctaggt
     3181 tctctcacta caaagtacat gtaaattacc attgtggcat ggttgtaact tgcaagggat
     3241 aatcagattg aatagacgat acataaaaat gtaaagtcac ggtaataata tatatcatca
     3301 aacctactcg tattttaact ttgcgtgtgt ttttgaacaa tctcatattt aacttatacc
     3361 cgtgaatctt gaacggatcg agataggttc ctcttgtcgg actatatcaa ggctaataag
     3421 ctagtccatt ccttacgact atatttaaag agaaagagag tgacaataat aggattgtac
     3481 attttgatac atattataag agtgacatca attagaaatg ggtcatacct tttgagacca
     3541 ctggctgcag acagcacaac caaaaagctt atcaactaat ctaaattagg gaattaataa
     3601 attaatttac caactaatca tgtagaagaa gtatgattta tttttgttgt ccaagcatca
     3661 agatgacgaa atgatattag aaaaaagaca agtatggaga aaaggtaaga aaaagtatac
     3721 gttagccaat aggactaatc attttatgaa tggctgtgaa atgaattgag ctgtagagca
     3781 aaaaaaaagt tccaaattat agaaacctat ctcgttatgt ttaggtttat gagttaagtt
     3841 tttgaaatga aaattacttt tctaatttgt aatttggacc acctgcatat caaagatctc
     3901 ttccccattt tgtttttaga gtaaatcata aaacccgtat actccaaagt ttcatattaa
     3961 ctgaaactat tttaacaaaa aaatcattca aaatgtgttt ttatcgacta aaaaaagtag
     4021 aaaaagtttt cttttgttga ttatgttttt tcatgattat accttttgag caataaaggg
     4081 ggaaacttgc tagttaatta actgaaaaat acgtttacta atttttgttt ttacctgcaa
     4141 ggctctgcct ctgattacaa aacaagatcg attgtgtttc ctgataataa aaataccttt
     4201 tttttttttg tagtagtagt agtagtagtt tgaagtttga accactatta cgtatctctt
     4261 ttgttgaaat caactatgag tccgcgggac ccacatagcg tcgtactttt ccgatcatcg
     4321 aataataaat catcacagat aaatcgacgg ctgtggatcc ccgggtggtc agtcccttat
     4381 gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt
     4441 cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga
     4501 aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg
     4561 taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg
     4621 ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa
     4681 tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta
     4741 tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca
     4801 gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt
     4861 ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca ccacgccgaa
     4921 cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc
     4981 tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca
     5041 acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga atccgcacct
     5101 ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa gccagacaga
     5161 gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt
     5221 cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag atgcggactt
     5281 gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa tggactggat
     5341 tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc tcgactgggc
     5401 agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta acctctcttt
     5461 aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa
     5521 cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc gtgacaaaaa
     5581 ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc
     5641 acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat
     5701 cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga
     5761 tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt tggaaacggc
     5821 agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc agccgattat
     5881 catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg
     5941 gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag
     6001 cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt
     6061 gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga agtcggcggc
     6121 ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg
     6181 caaacaatga atcaacaact ctcctggcgc accatcgtcg gctacagcct cgggaattgc
     6241 taccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     6301 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     6361 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     6421 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     6481 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa
     6541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6661 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6781 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     6841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6901 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     6961 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     7021 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     7081 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
     7141 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     7201 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     7261 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     7321 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     7381 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga
     7441 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga
     7501 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc
     7561 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg
     7621 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc
     7681 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
     7741 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
     7801 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
     7861 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
     7921 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
     7981 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
     8041 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
     8101 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
     8161 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
     8221 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
     8281 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
     8341 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
     8401 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
     8461 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
     8521 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
     8581 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
     8641 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
     8701 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
     8761 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     8821 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     8881 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     8941 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     9001 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     9061 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     9121 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     9181 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     9241 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     9301 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     9361 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     9421 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     9481 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     9541 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     9601 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     9661 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
     9721 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     9781 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     9841 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     9901 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     9961 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
    10021 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
    10081 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
    10141 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
    10201 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
    10261 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    10321 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    10381 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    10441 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    10501 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    10561 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    10621 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    10681 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    10741 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    10801 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    10861 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    10921 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    10981 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    11041 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    11101 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    11161 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
    11221 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
    11281 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
    11341 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
    11401 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
    11461 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
    11521 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
    11581 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
    11641 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
    11701 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
    11761 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
    11821 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
    11881 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
    11941 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct
    12001 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
    12061 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
    12121 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
    12181 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
    12241 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
    12301 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc
    12361 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc
    12421 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc
    12481 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc
    12541 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct
    12601 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
    12661 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
    12721 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
    12781 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
    12841 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
    12901 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
    12961 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
    13021 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
    13081 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
    13141 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
    13201 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
    13261 cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc tcggtcttgc
    13321 cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca
    13381 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
    13441 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
    13501 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
    13561 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
    13621 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
    13681 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
    13741 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
    13801 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
    13861 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
    13921 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
    13981 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
    14041 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca cgcttcccga
    14101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    14161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    14221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    14281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    14341 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    14401 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcca
    14461 gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa
    14521 aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt
    14581 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
    14641 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
    14701 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
    14761 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
    14821 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
    14881 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
    14941 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
    15001 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
    15061 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
    15121 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
    15181 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
    15241 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
    15301 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
    15361 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga
    15421 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
    15481 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
    15541 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
    15601 ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc tgtggttggc
    15661 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
    15721 tctaataaac gctcttttct cttag
//
