LOCUS       sequence-345744-       17862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17862)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 17862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL3:GUS
FEATURES             Location/Qualifiers
     source          1..17862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2391..2396,2397..2414,2415..2421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2434..2456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2453..2469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2453..2469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6496..8307
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      8378..8630
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8639..8656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8639..8655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8648..8670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8795..8814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9005..9026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9273..9297
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10893..12041)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12340..13134)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13326..14093
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14716..15427
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15554..15925)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            15958..16067
                     /note="incP origin of transfer"
     primer_bind     16290..16309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16543..16560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(16748..17398)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gctctgaggg aggcggttcc
     1921 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg
     1981 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     2041 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     2101 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     2161 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct
     2221 caatcggttg aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2461 agctatgacc atgattacgc caagcttgca tgcctgcagg tcgacgagta aaacaataat
     2521 aacggcggtt aaaaatagtt tttttaaaaa aatgctcaaa cgtgtgggtg ggttctctca
     2581 gttttggtga tgctagatta ctttattggg cttagaggaa aggcccaaac atcagaagat
     2641 aaaacccaag tttgtggttt gctagcttat aggttcatct tggttatgtc gcttattaac
     2701 cactagtttt ggtgtgatgt atttaggaat tattatgcag cgtgatagaa tgtttatgtt
     2761 tctgttgagg aaagaatatg atggtaaata ttactgcgaa aataagattg acgttgacaa
     2821 cctgaaacaa tgaattaaaa aattataaga atgttaacgt acggttgatg caatgagtgt
     2881 aagcttgtaa agagttgatg caatgaattt taacggtcaa tcatcagatt cataatatcg
     2941 atgttcatgt tacgtccata aaaaactgag gtatgggcaa ataataataa gaaaattata
     3001 aagcaaagaa atgagagtga aacatatcct gtgtgttcat agtttcatga atgaattatg
     3061 taattgtcaa cggtggatca atcattgaat ttggtggata tctatatttg tacacatgta
     3121 taaacgaatg cattccctca aagatattat ttggacagat tgacaacaca catagctaga
     3181 ttatctatat aaatcatacg ataacagaat aaaaatgtaa ttgagaatag agtaaatcaa
     3241 atctgcatca tatatataca gctcatatga attagagaat aaacaataga aaacaaacat
     3301 cttggtcgct tttgttttat atacgtttcg atgtattatg tatatgtaca catgcaaaca
     3361 taaactgtcc agtgcagaga ttcttatttc tttacagttt gtaatatgag gaaaaaaaaa
     3421 acatatatat acttaattag agcagctttt ggacatggtt ttagatttat atgcaaatgg
     3481 tcaaatgtat taaaacaatt aattatattt ttcgacgaaa taattgattt attctgggat
     3541 atattttggc gatcttgatc attataaaac ttttctttgt tgcttagaat atttatctgg
     3601 aaggacagtt ggaccaacga gacagaataa aaatcttctt tctacatcaa atttatttat
     3661 aaacacgggt ttatagtttg aaaaagtaca cttttaaaca cgcccaacgc atttgtttgt
     3721 catatttgtt attcgtgtca tgtatagaac ttgtataaac cttgtattta cgattcactg
     3781 gatttttgtg gtgacggtgg agtacttaaa aagtaaatat caaaagaaat tacacgtatg
     3841 agataatcag aacattgact tgcttaatta caattatggt tatcaattta tcattataat
     3901 gtttgatgtt gacaaagcat catttcacga acaaaatgac taaacaatca gcagaattat
     3961 tcaaccgctc gcgtataatg ttaatattca atcatagaaa ttgttccttc gacgacaatt
     4021 aacatttaat attagtacat ttcaggtcgg ttccacgtct tcataatgct aattaatcct
     4081 aaccgagaaa agttctcagt attgaccgta gttatttaac ttgagatata tcactgttta
     4141 ctaagcttaa gttttaatta tttgcataat caattcatat acaggattta aaattaaggg
     4201 tttattagaa aagtagacac tttttcaaac ataatttaca aactaggtag ttttttttta
     4261 aattagagaa ataaacactt tttttacttt ttgtgagata gaggatttgt gtaataaact
     4321 acaagacaaa atatatatca aatactaaaa tgccctttat attaagagat aagagaaaaa
     4381 atatatggga ccacttttct ttaaaacatt tcctctttat ttttattttt attttatttt
     4441 ttttaaatat acttttaaaa ataacttttt tttttaatat tataatgact tttatattta
     4501 tatatcatca atctcatgta ttcgtacacc agtattttaa acatagtttt gtacactaac
     4561 atttgtacac cagattatca tatacaaaaa aattattata atatctcata aatcattaaa
     4621 tacatcgatt tgtctcatat ttataaattg aagtaattat tgttcataag attaatacaa
     4681 acatgatagt aatgtaaact ataggtaata ctaaatatga ttgtgtacac catcatgtac
     4741 accattaaat tgaaatatga tgatacatac cattaaattt gaagtaatat ttatacattt
     4801 ctacgtagtg tacactaatg tttgtacact tctttatata ccaaattcgt acactatttt
     4861 ataaacatcg ttttggacat tatcacttga caaaaattgt tctctcttta tgtatgtggt
     4921 gtacaaaata ggaaggtgta caatattgta gacggcgcac gaggcggtgt atggtgtgac
     4981 ctattatcta gtcgttttat gtatacctat atacactata ttttacacat ctttgtacac
     5041 catcatatta ggtagttgta cacaactata tattttcgtt tgtacggtga cgtacactat
     5101 catatatggt atatcacttc tcacattaat attatacatt acttcttaca ttatcgtcgt
     5161 acactactat ttgtacacca ttttttacac tatcgtgtac accgtcggat tgagattaaa
     5221 aaaaaagtgt tacgtatttt cgaaatataa ctaaatttag actttttaga ctcacataaa
     5281 agttttaaag attaatttga ttatgtggta atttttttat gaaaaattac tatcaaataa
     5341 agtaaaaata ttgaagtaaa aagaagaaag ataaaaagta ggttatgaca agattttttt
     5401 ctttatcttg ttacataaca aaacaataaa aggttatata agttatttac taaaatctct
     5461 attagacaaa gaattgtatt atagacaaaa gagaaaagtg tctatttcac ctatttttaa
     5521 aaaaactacc tagtttacaa attatttttg aaaaaagtgt ctagtttgcc acttatctct
     5581 aaaactaatc cggtggataa actaattaag attaaggtga tgtgaatata catgaaatga
     5641 ccttagtaaa atattccttt cgcctttgat atatttgcca tgtgcggaat ctttattggc
     5701 ttcatagtta cacaaatgtt gcatggaatt taaattagag aagaaacctt ttgataacgt
     5761 tggggatatc ccaatcgtgc caacccaact attccaattt tacatttaca tgtctaaaac
     5821 taaaaggttc agcaagaaaa attattttcg atgcaaaaat aaacaggttt agctttaaaa
     5881 gtatcaaaca tataataaaa aagtttatat aaaaacatac aatatagtgt gaggcaagca
     5941 ttgtgctttt atttaagtta attttcattt tttcttacgt gttttatagt attaattacc
     6001 aatggaagat ttgaccaagg aagttagaaa gggggaaatt ttacccagat ttttattgtt
     6061 agtcaacttg aattaattaa ctttccagtt ttgaactatt ggaaattata atatcagctt
     6121 tgacgcagaa ttaaaggaaa tacgtgtgtg acttattaaa acaaaacaaa aatgggacca
     6181 tctttttggt gttgttgttc atattagctc attttatgta agtaaactta atgagacttc
     6241 catgactata agtaataaaa ataaaaataa aaattagcat gatgatttag gaataatatc
     6301 tagaggttat aattagcata cctttacatt aataatttat tttttttcca aaccttgtat
     6361 aaattagagc tcatagacag ctacacaaac aagaagaaaa atagtatata taaataaata
     6421 aaaatcaata cgtgacataa ctcaaaattc caaccaacaa tttttttttt tggatccccg
     6481 ggtggtcagt cccttatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc
     6541 gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca gcgttggtgg
     6601 gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc
     6661 gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga agtctttata
     6721 ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc
     6781 aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa
     6841 gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac
     6901 aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag
     6961 aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg
     7021 ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa
     7081 gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt cagcgttgaa
     7141 ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg gactttgcaa
     7201 gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca
     7261 gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg gtcagtggca
     7321 gtgaagggcg aacagttcct gattaaccac aaaccgttct actttactgg ctttggtcgt
     7381 catgaagatg cggacttgcg tggcaaagga ttcgataacg tgctgatggt gcacgaccac
     7441 gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc ttacgctgaa
     7501 gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc
     7561 ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac
     7621 agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat taaagagctg
     7681 atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat
     7741 acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac gcgtaaactc
     7801 gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc
     7861 atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc
     7921 ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa
     7981 ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca
     8041 atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc
     8101 gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg
     8161 acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc
     8221 aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa
     8281 aaaccgcagc agggaggcaa acaatgaatc aacaactctc ctggcgcacc atcgtcggct
     8341 acagcctcgg gaattgctac cgagctcgaa tttccccgat cgttcaaaca tttggcaata
     8401 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     8461 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     8521 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     8581 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattcac
     8641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8761 cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt cccttccttt
     8821 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8881 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8941 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9001 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     9061 gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg
     9121 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     9181 agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc
     9241 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     9301 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     9361 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     9421 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     9481 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat
     9541 catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga
     9601 caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg
     9661 aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa ctcccctatc
     9721 cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg cctgatgcat
     9781 ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa caactgtagg
     9841 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
     9901 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
     9961 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
    10021 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
    10081 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
    10141 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
    10201 acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc
    10261 cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat
    10321 attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac
    10381 gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg
    10441 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
    10501 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
    10561 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
    10621 gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa
    10681 atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt
    10741 taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg
    10801 aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct
    10861 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
    10921 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
    10981 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
    11041 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
    11101 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
    11161 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
    11221 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
    11281 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
    11341 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
    11401 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
    11461 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
    11521 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
    11581 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
    11641 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
    11701 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
    11761 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc
    11821 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
    11881 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
    11941 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
    12001 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
    12061 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
    12121 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
    12181 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
    12241 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
    12301 tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa
    12361 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
    12421 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    12481 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    12541 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    12601 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    12661 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    12721 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    12781 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    12841 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    12901 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    12961 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    13021 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    13081 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    13141 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    13201 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    13261 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
    13321 ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
    13381 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
    13441 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
    13501 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
    13561 atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt
    13621 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
    13681 acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc
    13741 agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg
    13801 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
    13861 tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg
    13921 taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc
    13981 tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag
    14041 tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt acccaattat
    14101 gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt
    14161 ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg ccttgccccc
    14221 tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt atcagtattg
    14281 tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc ttggtagctt
    14341 tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt ttcatacctc
    14401 ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga
    14461 gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca
    14521 tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc
    14581 cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc
    14641 ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg
    14701 caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc
    14761 cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
    14821 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
    14881 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
    14941 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
    15001 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
    15061 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
    15121 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
    15181 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
    15241 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
    15301 ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
    15361 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
    15421 aaggcgctcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct
    15481 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta
    15541 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa
    15601 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc
    15661 gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt
    15721 cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt
    15781 agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt
    15841 cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc
    15901 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg
    15961 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
    16021 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
    16081 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
    16141 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
    16201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    16261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    16321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    16381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    16441 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    16501 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    16561 gaagcggaag agcgccagaa ggccgccaga gaggccgagc gcggccgtga ggcttggacg
    16621 ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa
    16681 gggggagggg atgttgtcta catggctctg ctgtagtgag tgggttgcgc tccggcagcg
    16741 gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt
    16801 tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt
    16861 cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc
    16921 cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga
    16981 agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga
    17041 ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat
    17101 ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga
    17161 tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca
    17221 gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg
    17281 ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga
    17341 cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc
    17401 ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc
    17461 gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacatacc
    17521 cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc
    17581 ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg
    17641 cattctgctg gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct
    17701 gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg ccagatctgg
    17761 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    17821 tttaaatatc cgattattct aataaacgct cttttctctt ag
//
