LOCUS       sequence-345748-       14482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 14482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL5:GUS
FEATURES             Location/Qualifiers
     source          1..14482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2391..2396,2397..2414,2415..2421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2434..2456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2453..2469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2453..2469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3116..4927
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      4998..5250
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5259..5276)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5259..5275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5268..5290)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5415..5434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5625..5646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    5893..5917
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7513..8661)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8960..9754)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  9946..10713
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      11336..12047
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(12174..12545)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            12578..12687
                     /note="incP origin of transfer"
     primer_bind     12910..12929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13163..13180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(13368..14018)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gctctgaggg aggcggttcc
     1921 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg
     1981 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     2041 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     2101 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     2161 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct
     2221 caatcggttg aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2461 agctatgacc atgattacgc caagcttaaa gatttgaatc acctgattgc tttaataacc
     2521 aggaactggg gagagagaaa aggagatgag aagagtcaat agaggattga gaattaacat
     2581 agattagaca accattgatt taaatctcta tggtcatttc tccaaaaaac ccaattatag
     2641 attttggatg cctttgttta atccttcttt tctggatatt ttcagttatt tttgccttga
     2701 tctaatatta tttttactga gataattacc caatagcacc tttaaaactt gaggtaacta
     2761 tttttgtatt tacttaatta atcatacata ctacatatga ttattaaacg tagtatatat
     2821 caagtctcta ggtctattga aaataattat catacaagta attaaaaaaa ttccccatgg
     2881 ggtcagaagc taattaatgg cgggactctc tgttccccca aaagaatttg aagacatgac
     2941 tataaaaaaa aaagaatgaa acaattttat tttggactcc tgatctgtga aagactgaaa
     3001 cagttcatac gcaaactgat ataaaatcca aaatgttatc tctaagaata tggtccgtga
     3061 tcaataatac agatctaaaa ttcaccaact tggatccccg ggtggtcagt cccttatgtt
     3121 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
     3181 tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag
     3241 ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa
     3301 ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca
     3361 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca
     3421 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
     3481 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
     3541 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
     3601 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac
     3661 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
     3721 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
     3781 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
     3841 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
     3901 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct
     3961 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
     4021 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
     4081 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
     4141 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
     4201 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
     4261 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca
     4321 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
     4381 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
     4441 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
     4501 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
     4561 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
     4621 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag
     4681 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
     4741 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
     4801 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
     4861 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
     4921 acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac
     4981 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5041 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     5101 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     5161 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     5221 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     5281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5401 ctgaatggcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5521 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5641 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     5701 gatttcggaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     5761 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     5821 gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     5881 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     5941 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     6001 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     6061 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     6121 ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc
     6181 gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa
     6241 ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat
     6301 ttctttagaa gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac
     6361 aggtcgggga cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag
     6421 atctggggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     6481 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     6541 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     6601 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     6661 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     6721 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     6781 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     6841 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     6901 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     6961 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     7021 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca
     7081 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga
     7141 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc
     7201 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct
     7261 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc
     7321 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca
     7381 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt
     7441 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga
     7501 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     7561 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     7621 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     7681 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     7741 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     7801 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     7861 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     7921 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     7981 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     8041 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     8101 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     8161 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
     8221 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
     8281 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
     8341 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
     8401 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
     8461 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
     8521 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
     8581 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
     8641 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
     8701 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
     8761 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
     8821 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
     8881 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
     8941 gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat tttattttct
     9001 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
     9061 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
     9121 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
     9181 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
     9241 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
     9301 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
     9361 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
     9421 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
     9481 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
     9541 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
     9601 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
     9661 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
     9721 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
     9781 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     9841 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
     9901 gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac
     9961 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
    10021 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
    10081 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
    10141 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
    10201 ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
    10261 aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa
    10321 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
    10381 tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
    10441 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
    10501 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
    10561 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
    10621 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
    10681 ataaaacact atcaataagt tggagtcatt acccaattat gatagaattt acaagctata
    10741 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
    10801 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
    10861 atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc
    10921 tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata
    10981 gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac
    11041 ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact
    11101 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg
    11161 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg
    11221 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg
    11281 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc
    11341 atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc
    11401 gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
    11461 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
    11521 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
    11581 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
    11641 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
    11701 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
    11761 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
    11821 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
    11881 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg
    11941 gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc
    12001 cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga aaggcgctcg gtcttgcctt
    12061 gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg
    12121 ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc
    12181 tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg
    12241 atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg
    12301 gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc
    12361 tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc
    12421 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
    12481 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
    12541 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
    12601 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
    12661 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
    12721 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
    12781 atcgctataa tgaccccgaa gcagggttat gcagcggaaa agcgccacgc ttcccgaagg
    12841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    12901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    12961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    13021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    13081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    13141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgccagaa
    13201 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
    13261 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
    13321 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
    13381 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
    13441 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
    13501 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
    13561 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
    13621 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
    13681 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
    13741 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
    13801 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
    13861 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
    13921 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
    13981 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
    14041 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
    14101 ggaggctggt ccggaggcca gacgtgaaac ccaacatacc cctgatcgta attctgagca
    14161 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
    14221 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
    14281 cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
    14341 ggccaatctt gctcgtctcg ctggccggcg ccagatctgg ggaaccctgt ggttggcatg
    14401 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    14461 aataaacgct cttttctctt ag
//
