LOCUS       sequence-345759-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human TREM2 at the N-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TREM2_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 agatggcggg cattgcagct ggtggagggt ctgaatacag ctgtgaggat agtgatccct
       61 gggctaggct ctgcaaggaa actgagcagt gcagggcctt accagcccca accatctggg
      121 ggccaccctg gctggcacca gcaggagggt gggctggctt ctcagaggtc tgggagactc
      181 agcctccttc tgccagggct gcagtggccg actcctcctc ccctctgtcc ccaccctgca
      241 ccgcctccag accccagtcc tgactattgc ttaatcccca ggagcccagt tcctgtgggc
      301 agcgcctgac atgcctgatc ctctcttttc tgcagttcaa gggaaagacg agatcttgca
      361 caaggcactc tgcttctgcc cttggctggg gaagggtggc atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcgagcctc tccggctgct catcttactc tttgtcacag gtaggatccc
     1381 ctccgtgccc tgtgatgcct tctctccctc actttgagca tgtggttggg gcgcgggtgg
     1441 tgggccctgg ggtgaattat gaaggcggtg gcagtcctcg catgcctgag tgccctgttc
     1501 tgcactcctc tcctccccac cctgcccata ttcttcacac cctccttccc tccccagtat
     1561 caaaacctac cctcagactc tttctgcatg tggacatgta aggggctggt ggactctggt
     1621 tgcattggaa gggaaggagt gctaacagtg gcatcccagg cactagctgg cagttggggg
     1681 aagctttggg gggcactggc actggtaata gcctctgaaa ttataagcca cta
//
