LOCUS       sequence-345766-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human KCNC3 at the N-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCNC3_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ccctctaccc cgcccgcact tttttcagtt cagtaaggtc tctctctatc gtatctagcc
       61 ccgccttccc ctggctacca gtgctgccga acacaattag tagtaaccgg tcccgcctcc
      121 cttgacgccc cgccccgccc ctcctccctg ctctcttaga aagagccaat cgctccgtct
      181 cattggcggc cccgactcgt ccagctgggt cgcgttagag tgcccgcccc actcctccca
      241 gccacagccc cgccccacct cccccaggcc aatcagtttg gttcctctcc cctaagccac
      301 gcccccgcta cctcgctcct cctcccaccc aatcccgtcg gtctctcccc ccgtgtcccc
      361 gcccctgcgt ccccgcccct cccgccccgc cccccgtcca atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcctgagct cagtctgcgt ctcgtccttc cgcgggcgcc agggggccag
     1381 caagcagcag ccggcgccac cgccgcagcc gcccgagtcc ccgccgccgc caccgctgcc
     1441 gccgcagcag cagcagcctg cgcagcccgg ccccgccgcg tccccggcgg gccccccggc
     1501 accccgcggg cccggggacc ggcgcgccga gccatgcccc gggctgccgg cggcggccat
     1561 ggggcggcac ggcggcggcg gtggcgacag cggcaagatc gtgatcaacg tgggcggcgt
     1621 gcgccatgag acgtaccgct cgacgctgcg caccctgccg gggacgcggc tggccggcct
     1681 gacggagccc gaggcggcgg cacgcttcga ctacgacccg ggcgccgacg agt
//
