LOCUS       sequence-345775-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human ATP13A2 at the 
            N-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ATP13A2_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 catggagagc tggaccagca cgcacagtcc ctaaccactg ggacgtgctg gcgggggcta
       61 cctccgtgag gtgtgtgtct ccggtcgccc cgcccccggt gtgtgcggag gagcaggcgg
      121 ggactacaag tcccggcagc cccggcgcgg gcgctgcgag ggccgcagag ggccgggcgg
      181 ggcttgcggc gcgcacggag ggactgcggc agtgtcggag ccgcgccgag cctggtggcc
      241 caggtgcccc gcccgcgtca gccctgctcc agccccgcgc tagcccagcg cccctcgccc
      301 cgggccgtcc ggaccgcgcc cccgcccagg gccttgcgca cgccggggcc caggccgagg
      361 gccgcagcgc cggggccggc gatgagcgcg aggagccggc atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcagcgcag gtgagtgcgg agtccgggtc ggccccgagc ccggcgcggt
     1381 ccccggcccg ccagttgtcg tcaccccttg gagggagagg ggaggcgacg ccccccaccg
     1441 cggggtcgcg agcccaggaa gcgggacctg gttggggatc ctggcccgtc ccctcccttt
     1501 tgacggggac tcggccccgg tttcttccca gagacgccgg ggctggggat ggcgccgccg
     1561 ctcgcagccg gtccctcccc tcccctgcac ccctcagccg cgggattgga agccgggcgc
     1621 ttgtgcaaga gggtcctggg gccgtgcggg gacggggagg ccaccccacg cccacttaat
     1681 cggatcccac cccgagtgac ggatgtccag gtgggggaga cggctgcatc ctc
//
