LOCUS       sequence-345779-        1730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human LRRK2 at the N-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1730)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LRRK2_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ggcgtccgcc cggggtccgg tctaggcgtg cgtgggggcc acggtcacgg tcatcccagc
       61 caggcccggc tccagcagcc ccacggccgc cgccagagtt ctgcgcggcc cgtcgcctcg
      121 gcggagcctc tggcaggccc ctgagctcgt ttttggggcc tgagtggggg aggaggaagc
      181 cgagcaggag ggctccggag agggagggca acgcggggcg gggagctgcc tccttcctca
      241 taaacaggcg ggcgtgggcg ccgatggggc ccgcggggag cgctggctgc gggcggtgag
      301 ctgagctcgc ccccggggag ctgtggccgg cgcccctgcc ggttccctga gcagcggacg
      361 ttcatgctgg gagggcggcg ggttggaagc aggtgccacc atggctgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcgctagtg gcagctgtca ggggtgcgaa gaggacgagg aaactctgaa
     1381 gaagttgata gtcaggctga acaatgtcca ggaaggaaaa cagatagaaa cgctggtcca
     1441 aatcctggag gatctgctgg tgttcacgta ctccgagcac ggtaatcact tgaaaataaa
     1501 ctgtgctttt atttttgcaa actttctccc cctccttaca tttgcaaatt ttgtcctcct
     1561 ccccttgacc ctgctcaaac ccggactctt aaggagccgc aaactcccat atcctttcct
     1621 tagggcagaa agcagctgag aatttcagga aggtcttcac ctttttgact tttctccccg
     1681 tttcagacta aaaaggaaag ggggtgctgt ggattgtgac tttgcttctt
//
