LOCUS       sequence-345789-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human CACNA1A at the 
            N-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CACNA1A_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ctcttttttt ttttttccgg agtgggtgga ctggggattg cctggattcc ttcctcggtt
       61 attttttgcc tcgcttctct ctcccctccc ccctcctccc cgggcccccg cgccccgccc
      121 ccgcaccctc ctcccgcccc tccttctccg gggtcagcca ggaagatgtc ccgagctgct
      181 atccccggct cggcccgggc agccgccttc tgagcccccg acccgaggcg ccgagccgcc
      241 gccgcccgat gggctgggcc gtggagcgtc tccgcagtcg tagctccagc cgccgcgctc
      301 ccagccccgg cagcctcagc atcagcggcg gcggcggcgg cggcggcgtc ttccgcatcg
      361 ttcgccgcag cgtaacccgg agccctttgc tctttgcaga atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcgcccgct tcggagacga gatgccggcc cgctacgggg gaggaggctc
     1381 cggggcagcc gccggggtgg tcgtgggcag cggaggcggg cgaggagccg ggggcagccg
     1441 gcagggcggg cagcccgggg cgcaaaggat gtacaagcag tcaatggcgc agagagcgcg
     1501 gaccatggca ctctacaacc ccatccccgt ccgacagaac tgcctcacgg ttaaccggtc
     1561 tctcttcctc ttcagcgaag acaacgtggt gagaaaatac gccaaaaaga tcaccgaatg
     1621 gccatatcct tttgcccgaa ccccagcagc agctgcgcct ccccctcctc cctccgcctc
     1681 ccctcttcca ggctgggaga gagacccggg ggttgatggg aggtggggag gag
//
