LOCUS       sequence-34579-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Trojer P, Li G, Sims RJ, Vaquero A, Kalakonda N, Boccuni P, Lee D, 
            Erdjument-Bromage H, Tempst P, Nimer SD, Wang YH, Reinberg D
  TITLE     L3MBTL1, a histone-methylation-dependent chromatin lock.
  JOURNAL   Cell. 2007 Jun 1. 129(5):915-28.
  PUBMED    17540172
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 1. 129(5):915-28."
COMMENT     Sequence Label: pASK/3xMBT
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             27..49
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             75..92
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagttcgaca aaaatctaga aataattttg tttaacttta agaaggagat atacaaatgg
       61 ctagcagagg atcgcatcac catcaccatc acggggccgg agaccgcggt cccgaattcg
      121 agctcggtac ccggggatcc gctagcaccc cagagagtga ggagtggagc agcagccagc
      181 ctgcaacagg tgagaagaag gaatgctggt cgtgggagtc ctacctagag gagcagaagg
      241 ccattactgc tccagtcagc ctcttccagg actcccaggc agtcactcac aacaagaatg
      301 gcttcaaact gggcatgaag ttggaaggca ttgaccctca acacccgtcc atgtacttca
      361 tcctcaccgt ggctgaggta tgtggctatc gcctacgcct gcactttgat gggtattctg
      421 agtgccatga cttctgggtc aatgccaact cccctgacat tcaccctgct ggctggttcg
      481 agaagacggg ccacaagctg cagcctccca aaggttacaa ggaggaggag ttcagctgga
      541 gccagtacct gcgcagcaca agagctcagg ctgcccccaa gcacctgttt gtgagccaga
      601 gccacagtcc cccacccctg ggcttccagg tgggcatgaa gctggaggct gttgaccgca
      661 tgaacccgtc ccttgtctgc gtggccagtg tgaccgatgt ggtggacagc cgcttcctgg
      721 tgcac
//
