LOCUS       sequence-345790-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human CHMP2B at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CHMP2B_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 tgacatcttt gacggttctg atgacgaaga agaaagccag gatattgtga atcaagttct
       61 tgatgaaatt ggaattgaaa tttctggaaa ggtatgaaca tcatcttttc ttagttggaa
      121 atagtttctg cctaccacgt ttgtcactta attgttttgt ttttactagg aggtgcatgg
      181 tttttatttc tgtttcaaaa gtaaattaaa cagacctctt tacagcacat cctgtatgtc
      241 ctagtccaaa tgtttcctaa tgcacgtttg tctttttcat tgtttaatat agatggccaa
      301 agctccatca gctgctcgaa gcttaccatc tgcctctact tcaaaggcta caatctcaga
      361 tgaagagatt gaacggcaac tcaaggcttt aggagtagat ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataatcaa aagaagtcat actattttgc ttacttataa ttatgtagta
     1381 taaactaagc acagtgcaga tttcttttac aaaacacatg tattttgcaa aaaaaaaaaa
     1441 aatgaagacc atgagtgaac agttgtttcc taacccatgg ctatttagaa tcttttgcca
     1501 aagaatgaca atgatgcaaa aatgggaaca gtttggattt taattagaac tgtttaggag
     1561 tgatgatgtg taaaaagttg acttctcttt tgcatggcac agagaaatta tattccttac
     1621 ttcatgtcag tttatgttct aaatcttttt cactgaatat aaaaatcttg ttaaatgcca
     1681 ttaggcacca acttaaagag ggttgtaaaa atattaaaag tatatcgtta attctg
//
