LOCUS       sequence-345796-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human GCH1 at the N-terminus 
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GCH1_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 gcaataagtg gaggggtccc gcctgatcct aggggccggc gcccttttcc ttccctccct
       61 gcgcttgcga acccctccgg gcgtctccgg agccgcgacc cctgctgggg accccggggg
      121 cgggaggccg aggggcgcgg cgagggccgt gacgcgaggc ggggccggcc aatgggagcg
      181 ctccgcggcg caggctgagc cgccgggcca taaaaaggag gcgcggccgg gctttccagc
      241 ctgtggccgc tcccggctcg gagtgtgatc taagcaggtc gcgtaccttc ctcaggtgac
      301 tccggccaca gcccattgtc cgcggccacc ggcggagttt agccgcagac ctcgaagcgc
      361 cccggggtcc ttcccgaacg gcagcggctg cggcgggtcc atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcgagaagg gccctgtgcg ggcaccggcg gagaagccgc ggggcgccag
     1381 gtgcagcaat gggttccccg agcgggatcc gccgcggccc gggcccagca ggccggcgga
     1441 gaagcccccg cggcccgagg ccaagagcgc gcagcccgcg gacggctgga agggcgagcg
     1501 gccccgcagc gaggaggata acgagctgaa cctccctaac ctggcagccg cctactcgtc
     1561 catcctgagc tcgctgggcg agaaccccca gcggcaaggg ctgctcaaga cgccctggag
     1621 ggcggcctcg gccatgcagt tcttcaccaa gggctaccag gagaccatct caggtcagtg
     1681 cgcccgcggg ctgctagagc gtgcgggcgg cgggggcggt gcttgcagga aac
//
