LOCUS       sequence-345804-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human RNF216 at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RNF216_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 agaggtcgct gccctggtcc caggctagat tccattgagg ttggtttaat gtttttctcc
       61 actaatcccc acatcccttt cagagaacac cttcaaacgc attggacccc cgctggagaa
      121 gcctgtggag aaggtgcaga gggtggaggc cctcccgagg cccgttccgc agaacctgcc
      181 acagccacag atgccaccct atgccttcgc gcacccaccc ttccccctgc ctcccgtgcg
      241 gcctgtgttc aacaacttcc cactcaacat ggggcctatc ccagccccgt acgtgccccc
      301 tctgcccaac gtgcgggtca actatgactt cggtcccatc cacatgcccc tggagcacaa
      361 cctgcccatg cactttggcc cccagccgcg gcatcgcttc ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataatggc cccgaatccc cattgagcag cacaaagccc gtttggggta
     1381 ggagtgtgga tggagaaccc tcccccaagg ctggtgtctg taccattgca tcctaagtca
     1441 gcttgaaggg taggctggtt ttcttcccac ccctttccta gaagggctac tgctcctgga
     1501 agagtggacg gatccataat aaagacgtcc caaatggtgg agttcggaga gagctgcgat
     1561 gtgaactgcc cctcccctcg catcccccag gccaccaacg gcagtccttc tgccttgtcc
     1621 atggcatagg ccatagacca ggtccctgct gctcacacct gggcctctcc tcggagccga
     1681 cccctgggta gcaaggcagc cgagagcatc tccctggagg ggcccacggt tgggcc
//
