LOCUS       sequence-345808-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human SGP11 at the N-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGP11_Halo_N_allele
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..1291
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 aaggaaatgg caccacccca gagcctgtaa gcgaacagca caataagaaa agcgaagaaa
       61 agaggcagga actgggttcc aagggagctg gaggcagctg gaccaagaag gcaaagagca
      121 atgccacaga gcgagaacgg ctgcaacggc ctgctacgct aagctaggcc ttcaagcatg
      181 ccagagcagt taagcagagt gggactggcg tcagggcaga tgtggctttg ctagagaggc
      241 tggtactgcc ccccacagga aacgaatgga atcgaccgga gacagccgag aggtctccgc
      301 gggagtcacg aggccgagcg ggagccgcta cgtacaccgc gcatgcccac gacgcagtca
      361 ggttccggcg aaagtgaccg gaagtaaccg ccgggccaag atggccgaga tcggcaccgg
      421 ctttcccttc gatcctcact acgtggaagt gctgggcgag agaatgcact atgtggacgt
      481 gggccccaga gatggaaccc ctgtgctgtt tctgcacggc aaccctacca gcagctacgt
      541 gtggcggaac atcatccctc acgtggcccc tacacacaga tgtatcgccc ctgacctgat
      601 cggcatgggc aagagcgata agcccgacct gggctacttc ttcgacgacc acgtgcggtt
      661 catggacgcc tttattgagg ccctcggcct ggaagaggtg gtgctggtta ttcacgattg
      721 gggcagcgcc ctgggattcc actgggccaa gagaaacccc gagagagtga agggaatcgc
      781 cttcatggaa ttcatcaggc ccattcctac ctgggacgag tggcctgagt tcgccagaga
      841 gacattccag gccttcagaa ccaccgacgt gggcagaaag ctgatcatcg accagaacgt
      901 gttcatcgag ggcaccctgc ctatgggagt cgtcagacca ctgaccgagg tggaaatgga
      961 ccactacaga gagccctttc tgaaccccgt ggaccgggaa cctctttgga gattccccaa
     1021 cgagctgccc attgccggcg agcctgctaa tattgtggcc ctggtggaag agtacatgga
     1081 ctggctgcat cagagccccg tgcctaagct gctgttttgg ggaacacccg gcgtgctgat
     1141 tcctcctgct gaagctgcca gactggccaa gagcctgcct aattgcaagg ccgtggatat
     1201 cggccctggc ctgaatctgc tgcaagagga caaccccgat ctgatcggct ccgagattgc
     1261 cagatggctg agcaccctgg aaatcagcgg aggaggaggt tctggaggcg gtggaagtgg
     1321 tggcggaggt agcgctgcag aggaaggggt cgcgagtgct gcttccgccg gcggtagctg
     1381 gggcaccgcg gccatggggc gggttctacc gatgctgttg gtgccagtcc ccgccgaggc
     1441 gatggggcag ctcggctccc gggcgcagct gcgcacacag ccggaggctc tggggagcct
     1501 gacggctgcg ggcagcctcc aagtgctttc tttgacgcct ggcagccggg gcgggggtcg
     1561 ctgctgcctg gagggcccct tctggcagta agtgctgagg gagagttggg ccgttggggg
     1621 agtccagagc ctaggctcgg ctgactgagc cccaagggct cacgccgagg cctccctgca
     1681 gccatagagg tggcgggaga agggtgccag ctgcaggaaa cgccccagag gcg
//
