LOCUS       sequence-34581-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for 
            blue-light-induced transcription activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pGal4BD-CRY2PHR
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..577
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 agacctgcaa ttattaatct tttgtttcct cgtcattgtt ctcgttccct ttcttccttg
       61 tttctttttc tgcacaatat ttcaagctat accaagcata caatcaactc caagcttgaa
      121 gcaagcctcc tgaaagatga agctactgtc ttctatcgaa caagcatgcg atatttgccg
      181 acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa
      241 ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac
      301 agaagtggaa tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga
      361 agaccttgac atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg
      421 attatttgta caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga
      481 gactgatatg cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga
      541 gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg ctcgaggcca ccatgaagat
      601 ggacaaaaag actatagttt ggtttagaag agacctaagg attgaggata atcctgcatt
      661 agcagcagct gctcacgaag gatctgtttt tcctgtcttc atttggtgtc ctgaagaaga
      721 aggacagttt tatcctggaa gagcttcaag atggtggatg aaacaatcac ttgctcactt
      781 atctcaatcc ttgaaggctc ttggatct
//
