LOCUS       sequence-345810-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human TMEM106B at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TMEM106B_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 gaacttttat atttttaaca gaagataatt gttttaaaat attacactta ttatgtgtaa
       61 ttatgtctac agggctcact cagctatcca tttttgttgt ctgttgggga aatactcctt
      121 aagaggattg tgtgcacaat attaagttat cattaatcaa atattctctt ctgggagata
      181 atttttatgt gttaaagtag tctcactatg gaaaaacttc taatataact attaaatgtc
      241 tctcctcact tacattattt ttagagttac tgtgacaaca acatactttg gccactctga
      301 acagatatcc caggagaggt atcagtatgt cgactgtgga agaaacacaa cttatcagtt
      361 ggggcagtct gaatatttaa atgtacttca gccacaacag ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataaaaac tggaagagat ggatttaaag aagaaatatc tattgatatt
     1381 tcctatactc tcaatgaaga ggtatttcct aataggagac cttaaattga acaaacctaa
     1441 agtttacact tctaagagta cagttaaaag tatgtggacc tgcagttctt gtaactctcc
     1501 actctgtgtt aatgatatat ttgtactagg atcttttact tgaatctaaa tttactggtt
     1561 gatttccttc tccagcctat cccctacagg gaaaagctga tacttcccct atagtacaat
     1621 aaataattat ttaaaagtca tagctccagt cactactgaa aacataattt tggtgataaa
     1681 cataatttga gaaacttaat ttctgaatgt ttttatagaa aattactgaa agtcta
//
