LOCUS       sequence-345812-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human VPS35 at the C-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: VPS35_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 tgagccgagc caattttgct tgttttaaag ggttgttttt tttttttttt ttttgatagt
       61 cagtaattgt tcaaactagg aattgtatcc ccatctttct tttttcataa ttactcaggt
      121 aattgatgag tgtaacagaa gctcctcaaa acagttttat taaattgcct ttcatttttt
      181 gtggtacgtg cttgatcatg aatttgtaca tattcttttg taggtaacaa ttcaggtttt
      241 aaaccagctt atccaaaaga ttcgagaaga cctcccgaat cttgaatcca gtgaagaaac
      301 agagcagatt aacaaacatt ttcataacac actggagcat ttgcgcttgc ggcgggaatc
      361 accagaatcc gaggggccaa tttatgaagg tctcatcctt ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataaaaag gaaatagctc accatactcc tttccatgta catccagtga
     1381 gggttttatt acgctaggtt tcccttccat agattgtgcc tttcagaaat gctgaggtag
     1441 gtttcccatt tcttacctgt gatgtgtttt acccagcacc tccggacact caccttcagg
     1501 accttaataa aattattcac ttggtaagtg ttcaagtctt tctgatcacc ccaagtagca
     1561 tgactgatct gcaatttaaa attcctgtga tctgtaaaaa aaaaaaaaaa aaaaaacaca
     1621 aaacccacaa gcacttatct tggctactaa tgaagctctc cttttttttg tttgtttgtt
     1681 tgcttcattg ttgattgtgt attttcttca ttcctgggga gtactaaccc aaaagc
//
