LOCUS       sequence-345815-       10170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a
            mRuby3 fluorescent protein in its C-terminus. Inserted in a 
            lentiviral backbone. Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Bakhache W, Partiot E, Lucansky V, Bare Y, BonaventurB V, Goujon C, 
            Bories C, Deffieu M.S, Gaudin R
  TITLE     Pharmacological perturbation of intracellular dynamics as a 
            SARS-CoV-2 antiviral strategy
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro
FEATURES             Location/Qualifiers
     source          1..10170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4701..5411
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    5418..5985
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5435..5987
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5579..5596)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5806..5825
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5999..6598
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5999..6018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6495..6515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6612..7200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6665..6685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7083..7094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7348..7528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7575..7593)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7661..7765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7766..7834,7835..8626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7984..8003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8797..9385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9539..9556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9796..9817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9832..9837,9838..9855,9856..9862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9870..9886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9875..9897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9894..9910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9894..9910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9960..9979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc gccaccatga
     3421 gcgataacgg cccccaaaac caacggaatg cccctcggat aacatttggt ggccctagtg
     3481 attccacagg aagtaaccaa aacggagaac ggtctggagc ccgctctaaa cagaggcgac
     3541 cacagggatt gccaaataac acagcgtcat ggtttacagc cctgactcaa catggaaagg
     3601 aggacttgaa atttccacga ggacagggtg taccaataaa cacaaacagt agtccagacg
     3661 atcagatcgg gtattataga agggccacga gacgcatcag aggtggagat ggtaaaatga
     3721 aagatctcag tccgcgctgg tacttttatt atttgggaac cggcccagaa gcgggcctcc
     3781 catatggagc caataaggac gggattatat gggtggctac agagggagcc ctcaatacgc
     3841 caaaagatca catcggaaca agaaaccctg ctaataatgc cgcaatcgtg ctgcagttgc
     3901 ctcaggggac gacgcttcct aaaggctttt acgcagaagg atcacgcggc ggcagccaag
     3961 catcctcaag gtctagttcc agaagtcgaa acagctccag aaactccaca ccagggtcca
     4021 gtaggggcac aagtccggcg cggatggcgg gcaacggcgg agacgccgca ctcgctctgt
     4081 tgctcctgga ccggctcaac caacttgaat ccaagatgag tggtaaggga cagcagcaac
     4141 aaggtcaaac cgtaaccaag aaaagcgctg cagaagcttc taaaaaacct cgacaaaaac
     4201 ggaccgctac gaaggcatat aacgttactc aagccttcgg gagacgaggg ccggagcaaa
     4261 cccaggggaa ttttggagat caggagctca tccggcaagg gacagattac aaacattggc
     4321 ctcaaatcgc tcagttcgcg ccgtccgcaa gtgctttttt cggaatgtct cgcatcggta
     4381 tggaagtaac accatccggg acatggctca cttataccgg cgccatcaag ctggacgaca
     4441 aagatccaaa tttcaaagat caggtgattc tcttgaataa acacatagac gcgtataaga
     4501 cgtttccacc caccgaaccc aagaaggaca aaaagaaaaa agctgatgaa acacaagcgt
     4561 tgccccagcg acagaagaag cagcaaaccg tcaccctcct gccagccgct gatctcgatg
     4621 acttctccaa gcaacttcaa cagtccatga gctcagccga ttctactcag gcgggaggtt
     4681 ctggtggttc tggtggttcc atggtgtcta agggcgaaga gctgatcaag gaaaatatgc
     4741 gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc acaggtgaag
     4801 gagaaggcag accgtacgag ggagtgcaaa ccatgaggat caaagtcatc gagggaggac
     4861 ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc cgtaccttta
     4921 tcaagtaccc ggccgacatc cctgatttct ttaaacagtc ctttcctgag ggttttactt
     4981 gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc
     5041 ttgaggatgg cgagctcgtc tacaacgtca aggtcagagg ggtaaacttt ccctccaatg
     5101 gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg atgtatccag
     5161 cagatggtgg tctgagagga tacactgaca tcgcactgaa agttgatggt ggtggccatc
     5221 tgcactgcaa cttcgtgaca acttacaggt caaaaaagac cgtcgggaac atcaagatgc
     5281 ccggtgtcca tgccgttgat caccgcctgg aaaggatcga ggagagtgac aatgaaacct
     5341 acgtagtgca aagagaagtg gcagttgcca aatacagcaa ccttggtggt ggcatggacg
     5401 agctgtacaa gtgaggatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5461 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5521 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5581 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5641 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5701 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5761 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5821 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5881 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5941 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     6001 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6061 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6121 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6181 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6241 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6301 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6361 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6421 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6481 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6541 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat
     6601 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6661 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7021 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7201 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7261 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca cctgcaggac
     7321 aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7381 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7441 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7501 ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc tagatcattc
     7561 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     7621 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7741 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7801 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7861 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7921 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7981 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     8041 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8101 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8161 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8221 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8281 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8341 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8401 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8461 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8641 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8701 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9361 gtcaggggag cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9421 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9481 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9541 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9601 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
     9661 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9721 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     9781 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9841 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9901 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     9961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10021 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    10081 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    10141 ttgttaaata tgtactacaa acttagtagt
//
