LOCUS       sequence-34582-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gal4 Activation Domain fused to CIB1 for 
            blue-light-induced transcription activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pGal4AD-CIB1
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..583
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEXNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 atccccacca aacccaaaaa aagagatctt taatacgact cactataggg cgagcgccga
       61 agctagcgcc accatgaatg gagctatagg aggtgacctt ttgctcaatt ttcctgacat
      121 gtcggtccta gagcgccaaa gggctcacct caagtacctc aatcccacct ttgattctcc
      181 tctcgccggc ttctttgccg attcttcaat gattaccggc ggcgagatgg acagctatct
      241 ttcgactgcc ggtttgaatc ttccgatgat gtacggtgag acgacggtgg aaggtgattc
      301 aagactctca atttcgccgg aaacgacgct tgggactgga aatttcaaga aacggaagtt
      361 tgatacagag actaaggatt gtaatgagaa gaagaagaag atgacgatga acagagatga
      421 cctagtagaa gaaggagaag aagagaagtc gaaaataaca gagcanaaca atgggagcac
      481 aaaaagcatc aagaagatga aacacaaagc caagaaagaa gagaacaatt tctctaatga
      541 ttcatctaaa gtgacgaagg aattggagaa aacggattat attcatgttc gtgcacgacg
      601 aggccaagcc actgatagtc acagcatagc agaacgagtt agaagagaaa agatcagtga
      661 gagaatgaag tttctacaag atttggttcc tggatgcgac aagatcacag gcaaagcagg
      721 gatgcttgat gaaatcatta actatgttca gtctcttcag ag
//
