LOCUS       sequence-34583-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for 
            blue-light-induced transcription activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pGal4AD-CIBN
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             190..210
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             226..567
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     573..592
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        573..591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taaaaaagtt tgctgtcttg ctatcnagta taaatagacc tgcaattatt aatcttttgt
       61 ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa
      121 gctataccaa gcatacaatc aactccaagc tttgcaaaga tggataaagc ggaattaatt
      181 cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccgccaa ttttaatcaa
      241 agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag caacggtccg
      301 aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc ctcctctaac
      361 gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga tggtaataat
      421 tcaaaaccac tgtcacctgg ttggacggac caaactgcgt ataacgcgtt tggaatcact
      481 acagggatgt ttaataccac tacaatggat gatgtatata actatctatt cgatgatgaa
      541 gataccccac caaacccaaa aaaagagatc tttaatacga ctcactatag ggcgagcgcc
      601 gaattcatga atggagctat aggaggtgac cttttgctca attttcctga catgtcggtc
      661 ctagagcgcc aaagggctca cctcaagtac ctcaatccca cctttgattc tcctctcgcc
      721 ggcttctttg ccgattcttc aatgattacc ggcggcgaga tggacagcta tctttcgact
      781 gccggtttga atcttccgat gatgtacggt gagacgac
//
