LOCUS       sequence-345848-        1923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SED1 biosensor for expression in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, 
            Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, 
            Covarrubias AA, Sukenik S, Dinneny JR
  TITLE     Intrinsically disordered protein biosensor tracks the 
            physical-chemical effects of osmotic stress on cells.
  JOURNAL   Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8.
  PUBMED    34521831
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-14"; volume: "12(1):5438. doi"; pages: " 
            10.1038/s41467-021-25736-8"
COMMENT     Sequence Label: pDRFLIP38-AtLEA4-5
FEATURES             Location/Qualifiers
     source          1..1923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1515..1526
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggtgagca aaggtgaaga actgtttacc ggtgttgttc cgattctggt tgaactggat
       61 ggtgacgtta atggtcacaa attttcagtt agcggtgaag gcgaaggtga tgcaacctat
      121 ggtaaactga ccctgaaatt tatctgtacc accggcaaac tgccggttcc gtggccgacc
      181 ctggttacca ccctgagctg gggtgttcag tgttttgcac gttatccgga tcacatgaaa
      241 cagcacgatt ttttcaaaag cgcaatgccg gaaggttatg ttcaagaacg taccatcttc
      301 ttcaaagatg acggcaacta taaaacccgt gccgaagtta aatttgaagg tgataccctg
      361 gtgaatcgca ttgaactgaa aggcatcgat tttaaagagg atggtaatat cctgggccac
      421 aaactggaat ataatgccat tcatggcaac gtgtatatca ccgcagataa acagaaaaac
      481 ggcatcaaag caaattttgg cctgaactgc aatattgaag atggttcagt tcagctggca
      541 gatcattatc agcagaatac cccgattggt gatggtccgg ttctgctgcc ggataatcat
      601 tatctgagca cccagagcaa actgagcaaa gatccgaatg aaaaacgtga tcacatggtg
      661 ctgctggaat ttgttaccgc agcaggtatt accctgggta tggatgaact gtataaagag
      721 ctcatgcagt cgatgaaaga aacagcttcg aatattgcag cttctgcaaa atctggcatg
      781 gacaaaacca aagctacctt ggaggaaaag gcggagaaga tgaagacacg agaccctgtt
      841 cagaaacaga tggctacaca ggttaaagaa gataagatca atcaagctga gatgcagaag
      901 agagaaacgc gtcagcacaa cgcggccatg aaagaagcgg ctggagccgg aaccggttta
      961 ggtttgggga cggccactca ctcgaccact ggacaagtcg gacacggcac tgggacacat
     1021 cagatgtcgg ctctgcctgg tcacggaacg ggacaactga ccgaccgcgt tgtggagggc
     1081 acggctgtga ccgacccgat tggaaggaac actggaactg gtcggacaac cgctcataac
     1141 acacacgttg gtggtggtgg tgccaccggg tacggaaccg gcgggggata tactggaaga
     1201 tctatggtgt caaaaggtga ggaactgttt acaggcgtgg tgccgatcct ggtagagctg
     1261 gacggggatg tgaatggcca taaattcagc gtttcaggtg aaggtgaggg cgacgccacg
     1321 tacggaaaac tgacactgaa attcatttgc acaacaggta aactgcctgt gccttggcct
     1381 acactggtga ccacctttgg ttatggtctg atgtgctttg ctcgctatcc tgaccacatg
     1441 aaacaacatg atttctttaa atctgccatg cctgaaggct acgtgcaaga gcgcaccatt
     1501 tttttcaaag acgatgggaa ttacaaaaca cgtgcggagg tgaaattcga gggcgataca
     1561 ctggttaacc gtatcgagct gaaaggtatc gacttcaaag aggacggaaa cattctgggt
     1621 cataaactgg aatacaacta caacagccat aacgtgtaca tcatggccga caaacaaaaa
     1681 aacgggatta aagtgaactt caaaatccgc cacaacatcg aagatggcag cgtgcagctg
     1741 gccgaccact atcaacaaaa cacaccgatc ggcgacggtc ctgtactgct gcctgacaac
     1801 cactatctgt catatcagag cgcactgtca aaagatccta acgagaaacg cgaccacatg
     1861 gttctgctgg aattcgtgac agccgctggc attacactgg gcatggacga gctgtacaaa
     1921 taa
//
