LOCUS       sequence-345856-        9715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses  three sgRNA cassettes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Quinn JJ, Jones MG, Okimoto RA, Nanjo S, Chan MM, Yosef N, Bivona 
            TG, Weissman JS
  TITLE     Single-cell lineages reveal the rates, routes, and drivers of 
            metastasis in cancer xenografts.
  JOURNAL   Science. 2021 Feb 26;371(6532). pii: science.abc1944. doi: 
            10.1126/science.abc1944. Epub 2021 Jan 21.
  PUBMED    33479121
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-02-26"; volume: "371(6532). pii: science.abc1944. doi"; 
            pages: " 10.1126/science.abc1944. Epub 2021 Jan 21"
COMMENT     Sequence Label: PCT62
FEATURES             Location/Qualifiers
     source          1..9715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..99)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..850
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     805..825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             868..1048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1095..1220
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1717..1950
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2135..2179
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2477..2594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(3271..3304)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3308..3328
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3553..3573
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3724..3743
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3987..4198
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4199..5137
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5094..5114
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5158..5754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5158..5177)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5654..5674
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5764..5817
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5830..6528
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(6548..6581)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6637..7225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6690..6710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7108..7119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7228..7244)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7245)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7754..7934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7996..8584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8076..8095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8755..9546,9547..9615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9378..9397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9616..9715,1..5))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
       61 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
      121 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
      181 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
      241 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
      901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1021 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     1081 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1141 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1201 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1261 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1321 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1381 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1441 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1501 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1561 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1621 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1681 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1741 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1801 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1861 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1921 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1981 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     2041 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2101 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     2161 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2221 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2281 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2341 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2401 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2461 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2521 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2581 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagtaccgg
     2641 gcccgctcta gacgtgaccg agcttgtctg cccgcgcagt ccactagaca gacgcgcaga
     2701 ggtcgggcgg gcctagggcc aatttcccat ggttcccttc atttgcatat atgatgtaat
     2761 aaggttatgg agactattaa acttagccct aatcaaacta tatgatgata atgtgtgtgg
     2821 tacaaaaggt cataacttat tatatacttt gaaacttaaa aaagggttac agtttagtca
     2881 ccataactgt aaaatttttt ctattcttag ctttatatag ttcttgagag gccatgttta
     2941 tggcaggagc tatcaattcg cgggtttaag agctaagctg gaaacagcat agcaagttta
     3001 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttccag
     3061 tctgactatc acgatggatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcacg
     3121 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct
     3181 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag
     3241 ctacatttta catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag
     3301 ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact
     3361 ataagtatcc cttggagaac caccttgttg gtcctttgta tgccgaaaaa gtttgagagc
     3421 taagcagaaa gctgcatagc aagttcaaat aaggctagtc cgtacacaac ttgaaaaagt
     3481 ggcagccgag tcggctgctt tttttgctcg aatctacact cagctatggc gcgccccaag
     3541 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     3601 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     3661 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     3721 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     3781 ttgtggaaag ccagaaacat ggttgggtca taacgatacc tcgtttcaga gctaagcaca
     3841 agagtgcata gcaagttgaa ataaggctag tccgtttaca acttgaaaaa gtggcacccg
     3901 agtcgggtgc tttttttctc gagtactagg atccattagg cggccgcgtg gataaccgta
     3961 ttaccgccat gcatgtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4021 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     4081 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     4141 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     4201 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     4261 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     4321 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     4381 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     4441 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     4501 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     4561 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     4621 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     4681 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     4741 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     4801 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     4861 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     4921 cctcgattag ttctcgcgct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     4981 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     5041 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     5101 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcta gcccaccatg
     5161 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     5221 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     5281 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5341 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5401 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5461 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5521 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5581 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5641 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5701 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg
     5761 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg
     5821 gtcgccacca gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     5881 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     5941 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     6001 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
     6061 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
     6121 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     6181 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
     6241 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
     6301 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat
     6361 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
     6421 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
     6481 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattaaga attcgtcgag
     6541 ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg
     6601 ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     6661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7021 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7201 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa
     7261 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7321 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7381 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7441 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7501 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7561 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7621 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     7681 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     7741 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7921 gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtt
//
