LOCUS       sequence-345864-        5306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1341:Dendra
FEATURES             Location/Qualifiers
     source          1..5306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     341..360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1545..1568
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1825..2625
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2750..3338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3239..3258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat tgccgttccc
       61 gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt cgaaatattg
      121 gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg
      181 catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc
      241 acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct atcagtgata
      301 gagtttgtcc tccctatcag tgatagatag gcttaaggag taatacgact cactataggg
      361 agaaggaggc cattatggct ctgcgtgagt tcagttcggt caaggtcggg gacaccctgc
      421 cggaaagagt catcacgctg actcgcggag atctggtcaa ctacgccggc gtctccggcg
      481 acctcaaccc gatccactgg gacgacgaga tcgccaagca ggtcggtctc gacaccgcga
      541 tcgcacacgg catgctgacc atgggcctcg gcggcggcta cgtcacctcc tgggtgggcg
      601 accccgccgc ggtcaccgag tacaacgtgc ggttcaccgc cgtggtgccg gttcccaacg
      661 acggtgtcgg cgcggagatc accttcaacg gccgggtcaa gtccgtcgat gccgaggaga
      721 agctggtcac catcgcgatc tcggccaccg ccggcggcaa gaagatcttc gggcgcgccg
      781 tcgccaccgc acgactcgcc aagcttggtg cggccgcggg caacacgcct ggcatcaatc
      841 tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct
      901 tcgtgatcga gggggagggt aagggtaagc cttacgaagg gacccagacc gccaacctca
      961 ccgtcaagga gggcgcccct ctcccattct cgtacgatat cctcactacc gcggtgcatt
     1021 acgggaaccg cgtgttcacc aagtaccccg aggatatccc tgactacttc aagcagagct
     1081 tcccggaggg atactcctgg gagcggacga tgaccttcga agacaagggt atctgtacga
     1141 tccgcagcga catctcgctg gaaggggact gtttcttcca gaacgtccgt ttcaagggga
     1201 ccaactttcc gccgaacggc ccggtcatgc agaagaagac cctcaagtgg gagccgagca
     1261 cggagaagct gcacgttcgg gacggcctcc tggtcggcaa catcaacatg gcgctgctgc
     1321 tggaaggcgg cggccactac ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg
     1381 tccagctgcc cgacgcgcac ttcgtggacc accgcatcga aatcctgggg aacgattcgg
     1441 actacaacaa ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc
     1501 aggtctggtt cgaaggcggc ggcggggaga acctgtattt ccaggactac aaggacgacg
     1561 acgacaagtg atttaaataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
     1621 taactagcgt acgatcgaat tcctgcagct cacggtaact gatgccgtat ttgcagtacc
     1681 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     1741 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     1801 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac
     1861 gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt
     1921 gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga
     1981 ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg
     2041 tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga
     2101 gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc
     2161 tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct
     2221 tactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc
     2281 ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc
     2341 tatggggtgc cgagacactg caccattctt caggatggca agttggtacg cgtcgattat
     2401 ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag
     2461 agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac
     2521 attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca
     2581 gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg
     2641 agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgggggatc
     2701 cactagttcc atgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2881 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg
     3181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3361 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3481 cgaccgagcg caacgcgtgc ggccgcggta cccggggatc ctctagagtc gaccaccaag
     3541 ggcaccatct ctgcttgggc caccccgttg gccgcagcca gctcgctgag agccgtgaac
     3601 gacagggcga acgccagccc gccgacggcg agggttccga ccgctgcaac tcccggtgca
     3661 accttgtccc ggtctattct cttcactgca ccagctccaa tctggtgtga atgcccctcg
     3721 tctgttcgcg caggcggggg gctctattcg tttgtcagca tcgaaagtag ccagatcagg
     3781 gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc acaagagttg cagacccctg
     3841 gaaagaaaaa tggccagagg gcgaaaacac cctctgacca gcggagcggg cgacgggaat
     3901 cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg accacatatg ggccggtcaa
     3961 gataggtttt taccccctct cggctgcatc ctctaagtgg aaagaaattg caggtcgtag
     4021 aagcgcgttg aagcctgaga gttgcacagg agttgcaacc cggtagcctt gttcacgacg
     4081 agaggagacc tagttggcac gtcgcggatg gggatcgctg aagactcagc gcagcgggag
     4141 gatccaagcc tcatacgtca acccgcagga cggtgtgagg tactacgcgc tgcagaccta
     4201 cgacaacaag atggacgccg aagcctggct cgcgggcgag aagcggctca tcgagatgga
     4261 gacctggacc cctccacagg accgggcgaa gaaggcagcc gccagcgcca tcacgctgga
     4321 ggagtacacc cggaagtggc tcgtggagcg cgacctcgca gacggcacca gggatctgta
     4381 cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg tcacagagat
     4441 gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc cgactgcccg
     4501 ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg acaagctgat
     4561 cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct
     4621 gacgcctgag gagctggaca tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc
     4681 atacatcctg gcgtggacga gcctccggtt cggagagctg atcgagcttc gccgcaagga
     4741 catcgtggac gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg
     4801 gaacaagatc gtcgttggca acgccaagac cgtccggtcg aagcgtcctg tgacggttcc
     4861 gcctcacgtc gcggagatga tccgagcgca catgaaggac cgtacgaaga tgaacaaggg
     4921 ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac
     4981 caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct
     5041 ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc
     5101 ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg
     5161 cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag
     5221 cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac
     5281 cacgcctggc cgcgagcgcc agcacc
//
