LOCUS       sequence-345866-        5723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0941:Dendra
FEATURES             Location/Qualifiers
     source          1..5723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     736..755
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        736..754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2357..2380
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2637..3437
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3562..4150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4051..4070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc
       61 cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat
      121 ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga
      181 gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc
      241 tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa
      301 cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac
      361 gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca
      421 ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca
      481 acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg
      541 ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag
      601 ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg
      661 tctctgacga gcgggagaac tccctatcag tgatagagtt tgtcctccct atcagtgata
      721 gataggctta aggagtaata cgactcacta tagggagaag gaggccatta tgcgccccgc
      781 catcaaggtc ggcctgtcca cggcttccgt ctacccgctg agaaccgagg cggccttcga
      841 gatcgcggca cgcctcggct acgacggtgt cgagctgatg gtgtgggccg aatcggtcag
      901 ccaggacatc gacgccgtcg agaaactgtc ggcgaactac ggcgtcccgg tgctgtcggt
      961 gcacgcgccg tgtctgctga tctcgcaacg cgtatggggc gccaacccga tcaccaaact
     1021 ggagcgcagt gtgtgcgcgg ccgagcagct cggtgcgcag accgtcgtcg tccacccgcc
     1081 gttccggtgg cagcgccgct acgccgaggg gttcagcgag caggtcgccg aactggaggc
     1141 gggcagtgac gtcatggtcg ccgtcgagaa catgttcccc ttccgtgcgg accggttctt
     1201 cggtgcgggc cagacctcga tcgagcgcat gcgcaaacgc gggggcaggc cgggaccggg
     1261 catctcggcg ttcgcgccgt cctacgaccc gctggacggc aatcacgcgc actacacgct
     1321 cgatctgagc cacactgcga cggcaggcac cgatgcggtg gacatggcgc ggcgcatggg
     1381 cgacgggctg gtgcatctgc acctggccga cggcaacggc gcctcgaccg acgagcacct
     1441 ggttcccggc cgcggcacac agccggtcgc ccaggtgtgc cggatgctcg ccgagagtga
     1501 tttcgccggg cacgtggtgc tggaggtgac cacgcagacc gcacgcacca acggcgagcg
     1561 tgaggcgctt ctgaccgagt cgctacagtt cgcccgcaag catctgctgc gcaagcttgg
     1621 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1681 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1741 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1801 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     1861 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     1921 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     1981 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     2041 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     2101 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     2161 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     2221 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     2281 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     2341 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2401 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2461 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2521 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2581 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2641 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2701 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2761 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     2821 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     2881 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     2941 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     3001 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     3061 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     3121 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     3181 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3241 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3301 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3361 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3421 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3481 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3961 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     4321 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4381 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4441 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4501 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4561 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4621 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4681 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4741 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4801 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     4861 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     4921 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     4981 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     5041 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     5101 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     5161 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     5221 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     5281 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     5341 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5401 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5461 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5521 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5581 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5641 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5701 cacatgaagg accgtacgaa gat
//
