LOCUS       sequence-345867-        6710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0954:Dendra
FEATURES             Location/Qualifiers
     source          1..6710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     717..736
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        717..735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3325..3348
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3605..4405
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4530..5118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5019..5038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg
       61 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
      121 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
      181 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
      241 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
      301 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
      361 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
      421 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
      481 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      541 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      601 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      661 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt aaggagtaat
      721 acgactcact atagggagaa ggaggccatt atgggcggag ccgacagtct caccaaggtg
      781 ggcggtgccg acgctgcggt tgcggtggtc ggtgcggccg agggttcggt cggtgcggcc
      841 gagagcccgg cgtccgaccc tcagccgcgt gagagctcga gccggtcgcg cagccgcaag
      901 gtcaagcccg gtcgcatcac gttcgtcggt tcgggccccg gtgacccggg cctgctgacc
      961 accagggcgc aggccgtgct cgcgcatgcc gagctggtct tcaccgatcc cgatgtgccc
     1021 gaggcggtgc tcgccctcgt cggcaccgat ctgcccccgg cggcgggtcc ggcgcccgcc
     1081 aagaagaacg acaaggccga caaggccacc gacaagtcgg acaagtccga caaggccgac
     1141 aaggccgatg acgccaagtc cgacgatccg gcggtcatcc ccggcggacc cgaggtgcgt
     1201 cccgcgctcg gcgacccggc cgaggtcgcc aagacgctcg cgtccgaggc gcgccacgga
     1261 tacaacgtcg tgcgcctgat cgcgggtgac ccgctgtcgg tggatgccgt gatcaccgag
     1321 atcaacgcac tggccaagac gcatctgacc ttcgagatcg tcccgggtct gcccgacacc
     1381 accgcggttc cgacgtacgc cggcctgccg ctggggtcgt cgcacaccgt cgccgacgtg
     1441 cgtggcgacg tcgactgggc cgcactggcg gccgcgccgg ggccgctgat cctgcatgcg
     1501 accgcgtcgc acctgccgga tgccgcccgc acgctcatcg agcacgggct cgtcgagaac
     1561 acgcccgcgg tcgtcacggc caacggcacc acctgccagc agcgttcggt cgagaccacg
     1621 ctggtggggc tcaccgacaa gaccgtggtg gagaagcccg cgggcagcga gctgttgggc
     1681 ccgctgaccg gaccgctggt ggtcaccatc ggcaagaccg tcgccaaccg cgccaagctg
     1741 aactggtggg agagccgcgc cctgtacggc tggaccgtgc tggtgccgcg caccaaggat
     1801 caggccggcg agatgagcga gcggctcgtc acgcacggcg ccctgccgat cgaggtgccc
     1861 accatcgccg tcgagccgcc gcgcagcccg gcgcagatgg agcgcgcggt caagggcctg
     1921 gtcgacggcc ggttccagtg ggtcgtgttc acctccacca acgccgttcg cgcggtgtgg
     1981 gagaagttca acgagttcgg tctggacgcc cgcgcgttct ccggcgtgaa gatcgcctgc
     2041 gtggggcagg ccaccgcgga caaggtgcgc gcgttcggga tcaatcccga actggtgccg
     2101 tcgggcgagc agtcctcgct cggcctgctg gatgaattcc cgccctacga cgaggttttc
     2161 gacccggtca accgtgtgtt gctgccgcgt gcggacatcg ccaccgagac gctggccgag
     2221 ggtctgcgtg agcgtggctg ggagatcgag gacgtcacgg cgtaccgcac cgtgcgtgcg
     2281 gccccgccgc ctgcgcacac ccgcgagatg atcaagaccg gtggtttcga cgcggtgtgc
     2341 ttcacgtcga gttcgaccgt gcgcaacctc gtcggcatcg cgggcaagcc gcacgcccgc
     2401 accatcgtgg cctgcatcgg accgaaaacc gcagaaaccg caatcgaatt cggtctgcgc
     2461 gtggatgtgc agcccgagac agccgcggtc ggaccgctgg tcgaggcgct cgccgagcac
     2521 gccgcccggt tgcgcgccga gggtgcgctc ccgccgccgc gcaagaagag ccgccgcagg
     2581 aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga ggacatgcgt
     2641 gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga gggggagggt
     2701 aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga gggcgcccct
     2761 ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg cgtgttcacc
     2821 aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg atactcctgg
     2881 gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga catctcgctg
     2941 gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc gccgaacggc
     3001 ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct gcacgttcgg
     3061 gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg cggccactac
     3121 ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac
     3181 ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa ggtcaagttg
     3241 tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc
     3301 ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg atttaaataa
     3361 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt acgatcgaat
     3421 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     3481 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     3541 ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg
     3601 actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct
     3661 catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc
     3721 cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc
     3781 tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc
     3841 gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg
     3901 gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca
     3961 ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg caagcccggt
     4021 cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa
     4081 cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg
     4141 caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg
     4201 tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc
     4261 agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg
     4321 tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg
     4381 cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag
     4441 gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc atgagcgtca
     4501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4921 ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc
     4981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc
     5281 ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct ctgcttgggc
     5341 caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc
     5401 gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct
     5461 cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg
     5521 gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat
     5581 gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg
     5641 gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt
     5701 ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct
     5761 cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga
     5821 gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac
     5881 gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca
     5941 acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg
     6001 aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg
     6061 accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc
     6121 tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc
     6181 gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct ctggtgcgtg
     6241 cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc
     6301 tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac ccgtgccgga
     6361 tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag gagctggaca
     6421 tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg gcgtggacga
     6481 gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac gacggcatga
     6541 cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca
     6601 acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc gcggagatga
     6661 tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca
//
