LOCUS       sequence-345868-        5150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0977:Dendra
FEATURES             Location/Qualifiers
     source          1..5150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     670..689
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        670..688
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1718..1741
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1998..2798
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2923..3511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3412..3431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga
       61 cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat
      121 ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc
      181 ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa
      241 aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag
      301 aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc
      361 ccgccgccgc caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc
      421 ccgctttgat cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc
      481 ggcacgatcc gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac
      541 gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg
      601 acgagcggga gaactcccta tcagtgatag agtttgtcct ccctatcagt gatagatagg
      661 cttaaggagt aatacgactc actataggga gaaggaggcc attatggttg ccgatgtgct
      721 cgcatacgtc gcggcccggc tggttctcgt cgcggtactc gccgcggtga tctacgtggt
      781 gggccggatg gtcgtcggtg acttcccgat cgtggtggct ttgctgttct cgttcgtgat
      841 cgcgctgcct ctgggcatct gggtgttcaa gccgctgcgt ctgcgtgcga cggcctccat
      901 tgcggaattc gacgagcgcc ggcgcaagga ccgtgcggat ctgcaggcgc ggcttcgtgg
      961 cgacgacccc aagaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1021 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1081 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1141 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1201 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     1261 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     1321 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     1381 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     1441 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     1501 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     1561 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     1621 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     1681 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     1741 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     1801 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     1861 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     1921 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     1981 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2041 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2101 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2161 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     2221 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     2281 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     2341 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     2401 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     2461 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     2521 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     2581 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     2641 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     2701 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     2761 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     2821 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     2881 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3061 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3301 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     3361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3661 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     3721 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     3781 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     3841 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     3901 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     3961 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4021 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4081 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4141 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4201 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4261 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     4321 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     4381 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     4441 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     4501 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     4561 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     4621 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     4681 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     4741 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     4801 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     4861 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     4921 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     4981 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5041 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5101 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt
//
