LOCUS       sequence-34587-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Carr HS, Cai C, Keinanen K, Frost JA
  TITLE     Interaction of the RhoA exchange factor Net1 with discs large 
            homolog 1 protects it from proteasome-mediated degradation and 
            potentiates Net1 activity.
  JOURNAL   J Biol Chem. 2009 Sep 4. 284(36):24269-80.
  PUBMED    19586902
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Sep 4. 284(36):24269-80."
COMMENT     Sequence Label: pEF-HA/Net1A
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             104..130
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgtgtggga ttgcttgctt acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagacacc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
      121 ggactatgca ggatccatgg tggcgcatga tgagatcgga ggtctcctgc ccattaaaag
      181 gacgatccga gtcttagatg ttaataacca gcccttccgg gagcaggagg agccaagcaa
      241 taagagagtt cggcctctag ctcgggtcac atctttggca aacttaatct ctcccgtaag
      301 aaatggagca gtcaggcgct tcggtcagac catacagtcg tttacccttc gtggtgacca
      361 cagatcccca gcctctgccc agaagtcatt cagcaggtcc accgtcccca cacccaccaa
      421 gagaagaagc agtgcactat ggtcggaaat gctagacatc aatatgaagg agtccttgac
      481 cactcgagag atcaaacgtc aggaggccat atatgaattg tcccgagggg aacaggactt
      541 gattgaggat ctcaagctcg ctagaaaggc ctaccatgac cccatgttaa agctgtctat
      601 catgtcagaa gaggaactca cgcacatatt tggagacttg gatgcttata tacctctgca
      661 cgaagatctg ttagcaagaa ttggagaagc aaccaagcct gatgggactg tggagcagat
      721 cggtcacatt ctcgtgaact ggttaccagg cctgaatgcc tacagaggct actgcagtaa
      781 ccagctggcg gccaaggctc ttctggatca gaagaaacaa gacccaagag tccaagactt
      841 cctccagcgc tgtctggagt ctcctttca
//
