LOCUS       sequence-345881-        5807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1075:Dendra
FEATURES             Location/Qualifiers
     source          1..5807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     600..619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        600..618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2305..2328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2585..3385
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3510..4098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3999..4018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
       61 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
      121 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
      181 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
      241 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
      301 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
      361 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
      421 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
      481 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
      541 gaactcccta tcagtgatag agtttgtcct ccctatcagt gatagatagg cttaaggagt
      601 aatacgactc actataggga gaaggaggcc attatgagca gccaagcagc ttcggacaac
      661 ccgttcgacc ccgcgatgtg ggaacgggtg cccggcttcg atgatctgac cgacatcacc
      721 taccaccgcc acgtgctcga cggcgcacgt cagcccaccg tgcgcgtggc cttcgaccgg
      781 cccgaggtgc gcaacgcgtt ccggccccac acggtcgacg agctgtaccg cgtgctcgac
      841 cacgcgcgga tgtcgtccga cgtcggcgtc atcctgctca ccggcaacgg cccgtcgccc
      901 aaggacggcg gctgggcgtt ctgctccggc ggcgatcagc gcatccgtgg gcgcacgggc
      961 taccagtacg cctcgggcga gacggccgag accgtcgacc cggcacgcgc gggccggctg
     1021 cacattctcg aggtgcagcg gctcatccgg ttcatgccca aggtcgtcat ctgcctggtg
     1081 aacggctggg ccgccggtgg tgggcacagc ctgcacgtca cgtgcgacct gacgctggcc
     1141 agccgtgagc acgcgcggtt caagcagacc gacgccgacg tgggcagctt cgacggcggg
     1201 ttcggcagcg cctacctggc gcgccagacc ggccagaagt tcgcgcgcga gatcttcttc
     1261 ctgggccgtg cctacgacgc gcagaccatg caccagatgg gcgcggtcaa cgaggtcgtc
     1321 gaccatgccg atctggagaa ggccgggctg cagtacgccg ccgagatcaa cggcaagtcg
     1381 ccgcaggcga tccggatgct caagttcgcg ttcaacctca tcgacgacgg cctggtgggc
     1441 cagcaggtgt tcgccggtga ggcaacacgt ctggcgtaca tgaccgacga agccgtcgaa
     1501 ggccgcgacg cgttcttgga gaagcgcgac ccggactgga gccgcttccc gcgctacttc
     1561 aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga ggacatgcgt
     1621 gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga gggggagggt
     1681 aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga gggcgcccct
     1741 ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg cgtgttcacc
     1801 aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg atactcctgg
     1861 gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga catctcgctg
     1921 gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc gccgaacggc
     1981 ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct gcacgttcgg
     2041 gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg cggccactac
     2101 ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac
     2161 ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa ggtcaagttg
     2221 tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc
     2281 ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg atttaaataa
     2341 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt acgatcgaat
     2401 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     2461 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     2521 ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg
     2581 actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct
     2641 catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc
     2701 cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc
     2761 tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc
     2821 gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg
     2881 gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca
     2941 ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg caagcccggt
     3001 cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa
     3061 cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg
     3121 caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg
     3181 tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc
     3241 agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg
     3301 tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg
     3361 cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag
     3421 gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc atgagcgtca
     3481 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3541 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3601 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3661 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3721 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3781 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3841 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3901 ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc
     3961 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4021 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4081 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4141 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4201 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc
     4261 ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct ctgcttgggc
     4321 caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc
     4381 gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct
     4441 cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg
     4501 gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat
     4561 gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg
     4621 gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt
     4681 ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct
     4741 cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga
     4801 gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac
     4861 gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca
     4921 acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg
     4981 aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg
     5041 accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc
     5101 tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc
     5161 gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct ctggtgcgtg
     5221 cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc
     5281 tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac ccgtgccgga
     5341 tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag gagctggaca
     5401 tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg gcgtggacga
     5461 gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac gacggcatga
     5521 cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca
     5581 acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc gcggagatga
     5641 tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga
     5701 ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct
     5761 acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgct
//
