LOCUS       sequence-345883-        5699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1047:Dendra
FEATURES             Location/Qualifiers
     source          1..5699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1220..1239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1220..1238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2817..2840
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3097..3897
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4022..4610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4511..4530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
       61 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
      121 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
      181 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact
      241 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
      301 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
      361 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
      421 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
      481 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga
      541 agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc
      601 gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca
      661 aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg
      721 cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct
      781 gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca
      841 gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg
      901 ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct agacggtgac
      961 cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca ccgtcacggc
     1021 cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg tgctgcgcag
     1081 tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga
     1141 agggtctctg acgagcggga gaactcccta tcagtgatag agtttgtcct ccctatcagt
     1201 gatagatagg cttaaggagt aatacgactc actataggga gaaggaggcc attatgactg
     1261 acgacccgct ggtcccgctg cccgccgcga cggtgatgct gatccgcgac gtgcaccgcg
     1321 agggacgtgc ggatctcgag gtgttcctga tgcgaaggca cgccgcgatg gacttcgtcg
     1381 ccggcgtgat ggtgttcccg ggcggtggcg tcgacgaccg tgaccgcaac gccgacatcg
     1441 cgtggtacgg acccgagccg tcatggtggg cccagcgcct cggcgtcgac gaggggctgg
     1501 ccgaggcgct cgtgtgcgcc gcggcgcgtg agacgttcga ggagtccggc gtgctgttcg
     1561 ccggccccca gaacgggccc ggtgaccccg gcatcatcgc cgacgcgtcg gtgtacggcg
     1621 acgcgcgcgc ggccctggcc aaccactcgc tgtcgttcgc cgacttcctg cgcgacgaga
     1681 agctggtgct gcgcgccgac ctgctgcggc cgtgggccaa ctgggtgacg cccaaagagg
     1741 agcgcacacg gcgctacgac acgtatttct tcgtcgcggc cctcccggag ggccagcgcg
     1801 ccgacggcga gaacaccgag accgaccggg cgttctggtg cacaccgaaa gccgggctcg
     1861 acgacttcga ggcggggaac accttcctgc tgccgccgac ctggacgcag ctggactccc
     1921 tcgacggccg cacggtggcc gaggtgctgg cggtcgaacg caagatcgtg acgatcgagc
     1981 ccagcctgtc gaccgagggc aacaactggg agatcgaata cttcgacagc gggcgctaca
     2041 acgctgcacg caagcatcgg tcgcccgggc aaaagcttgg tgcggccgcg ggcaacacgc
     2101 ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga
     2161 acggtcacgc cttcgtgatc gagggggagg gtaagggtaa gccttacgaa gggacccaga
     2221 ccgccaacct caccgtcaag gagggcgccc ctctcccatt ctcgtacgat atcctcacta
     2281 ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc cgaggatatc cctgactact
     2341 tcaagcagag cttcccggag ggatactcct gggagcggac gatgaccttc gaagacaagg
     2401 gtatctgtac gatccgcagc gacatctcgc tggaagggga ctgtttcttc cagaacgtcc
     2461 gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat gcagaagaag accctcaagt
     2521 gggagccgag cacggagaag ctgcacgttc gggacggcct cctggtcggc aacatcaaca
     2581 tggcgctgct gctggaaggc ggcggccact acctatgcga cttcaagacc acgtacaaag
     2641 cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg
     2701 ggaacgattc ggactacaac aaggtcaagt tgtacgagca cgctgtggca cgctatagcc
     2761 cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga gaacctgtat ttccaggact
     2821 acaaggacga cgacgacaag tgatttaaat aaccccttgg ggcctctaaa cgggtcttga
     2881 ggggtttttt gttaactagc gtacgatcga attcctgcag ctcacggtaa ctgatgccgt
     2941 atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga
     3001 atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat
     3061 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat
     3121 gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt
     3181 acccccggcg gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat
     3241 gggccacttg gactgatcga ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc
     3301 atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca
     3361 ccggaccttg gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc
     3421 catccatttg cctttgcggc agcggggcca caggcagagc agatcatctc tgatccattg
     3481 cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag
     3541 gtacttctcc tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg
     3601 gcaaaggttc cctatggggt gccgagacac tgcaccattc ttcaggatgg caagttggta
     3661 cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg
     3721 ctcaaggaga agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc
     3781 cgctcccgcg acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc
     3841 ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga
     3901 gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat tgctggggca
     3961 acacggggga tccactagtt ccatgagcgt cagaccccgt agaaaagatc aaaggatctt
     4021 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4081 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4141 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4201 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4261 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4321 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4381 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgagg
     4441 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4501 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4561 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4621 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4681 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4741 gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg tacccgggga tcctctagag
     4801 tcgaccacca agggcaccat ctctgcttgg gccaccccgt tggccgcagc cagctcgctg
     4861 agagccgtga acgacagggc gaacgccagc ccgccgacgg cgagggttcc gaccgctgca
     4921 actcccggtg caaccttgtc ccggtctatt ctcttcactg caccagctcc aatctggtgt
     4981 gaatgcccct cgtctgttcg cgcaggcggg gggctctatt cgtttgtcag catcgaaagt
     5041 agccagatca gggatgcgtt gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt
     5101 tgcagacccc tggaaagaaa aatggccaga gggcgaaaac accctctgac cagcggagcg
     5161 ggcgacggga atcgaacccg cgtagctagt ttggaagaat gggtgtctgc cgaccacata
     5221 tgggccggtc aagataggtt tttaccccct ctcggctgca tcctctaagt ggaaagaaat
     5281 tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa cccggtagcc
     5341 ttgttcacga cgagaggaga cctagttggc acgtcgcgga tggggatcgc tgaagactca
     5401 gcgcagcggg aggatccaag cctcatacgt caacccgcag gacggtgtga ggtactacgc
     5461 gctgcagacc tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg agaagcggct
     5521 catcgagatg gagacctgga cccctccaca ggaccgggcg aagaaggcag ccgccagcgc
     5581 catcacgctg gaggagtaca cccggaagtg gctcgtggag cgcgacctcg cagacggcac
     5641 cagggatctg tacagcgggc acgcggagcg ccgcatctac ccggtgctag gtgaagtgg
//
