LOCUS       sequence-345884-        5747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0988:Dendra
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     702..721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2347..2370
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2627..3427
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3552..4140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4041..4060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc
       61 aagatcggtc ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc
      121 gctcaggcag gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg
      181 atggcgatga agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg
      241 tccaagctgg ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc
      301 taaagagggg ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac
      361 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
      421 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
      481 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
      541 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
      601 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcagtgat
      661 agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac tcactatagg
      721 gagaaggagg ccattatggc cagtttcgcg cagtggatcg aaggcgcccg cccccgtacg
      781 ttgcccaacg ccgtatcacc ggtgatcgcc ggcaccggcg cggctgcctg gctggatgcg
      841 gccgtgtggt ggaaagcatt gctggcgttg gccgtctcgc tcgcgttgat cgtgggtgtc
      901 aacttcgcca acgactactc cgacggcatc cgcggcaccg acgatgtgcg gtcggggccg
      961 ctgcgcctgg tgggttcgaa actcgcgtcg ccccgcgcgg tgctgaccgc ggcgatcgtg
     1021 agcctggccg tcggcgcggt ggccggcctg gcgctggccg tgacgagcgc gccgtggctc
     1081 atcgccgtcg gcgcggtgtg catcgccggg gcgtggctgt acaccggcgg caagaagccc
     1141 tacggctacc agggcctcgg cgaggtcgcg gtgttcatct tcttcggtct ggtcgcggtg
     1201 ctcggcacgc agtacgtgca ggcgctgcgc gtcgactggg tcggtctggt gctcgcggtc
     1261 gcgatggggt cgctctcgtc ggccgtgctg gtggccaaca acctgcgcga catcccgacc
     1321 gacgcccaga ccggcaagat cacgctggcc gtgcgcctcg gcgacgtacg cacccgcgtg
     1381 ctgttccagg tgctggtcgg ggtcgcgatg gtgctgacac tcgtgctgat gctcgcgacc
     1441 ccgtgggccg cggcgggcct ggtggcgctg ccgctcgccg cgcgtgccgc cgcacccgtt
     1501 cgccgcggcc tcggcggcaa ggatctgatc cccgtgctgc gcgacaccgg cctgaccatg
     1561 ctggtgtggg cgctcgcggt ctcgatcccg ctggcgttcc acaagcttgg tgcggccgcg
     1621 ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt gcatatggag
     1681 ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa gccttacgaa
     1741 gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt ctcgtacgat
     1801 atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc cgaggatatc
     1861 cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac gatgaccttc
     1921 gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga ctgtttcttc
     1981 cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat gcagaagaag
     2041 accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct cctggtcggc
     2101 aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga cttcaagacc
     2161 acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga ccaccgcatc
     2221 gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca cgctgtggca
     2281 cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga gaacctgtat
     2341 ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg ggcctctaaa
     2401 cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag ctcacggtaa
     2461 ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc
     2521 cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac
     2581 caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt
     2641 ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc agcttctcaa
     2701 ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta gcgtccggcc
     2761 cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg gtccgggagg
     2821 gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc
     2881 gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc caaatgtaaa
     2941 gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc agatcatctc
     3001 tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg tccatgaact
     3061 cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc
     3121 cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc ttcaggatgg
     3181 caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct ttgccttggc
     3241 ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc atgcctttgc
     3301 tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg gccgagatcc
     3361 gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc
     3421 gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat
     3481 tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt agaaaagatc
     3541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3661 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3961 cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4201 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4261 gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg tacccgggga
     4321 tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt tggccgcagc
     4381 cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg cgagggttcc
     4441 gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg caccagctcc
     4501 aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt cgtttgtcag
     4561 catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt cagaagagtc
     4621 gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac accctctgac
     4681 cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat gggtgtctgc
     4741 cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca tcctctaagt
     4801 ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa
     4861 cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga tggggatcgc
     4921 tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag gacggtgtga
     4981 ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg
     5041 agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg aagaaggcag
     5101 ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag cgcgacctcg
     5161 cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac ccggtgctag
     5221 gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg gccgggatgg
     5281 gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg gtgatgaaca
     5341 cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag aaggcagccg
     5401 atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc gctgagatct
     5461 tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg ttcggagagc
     5521 tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag ctccgggtgc
     5581 gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag accgtccggt
     5641 cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg cacatgaagg
     5701 accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacg
//
