LOCUS       sequence-345903-        5867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1133:Dendra
FEATURES             Location/Qualifiers
     source          1..5867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     769..788
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        769..787
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2534..2557
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2814..3614
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3739..4327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4228..4247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatccgagcg cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt
       61 gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg
      121 ctacgccaag atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac
      181 gttcgccgct caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac
      241 tcctaggatg gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga
      301 ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac
      361 tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg
      421 ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg
      481 ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg
      541 aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg
      601 atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt
      661 gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat
      721 cagtgataga gtttgtcctc cctatcagtg atagataggc ttaaggagta atacgactca
      781 ctatagggag aaggaggcca ttatggtggc aggcgttgac ttcggcgatc cggcattcgc
      841 cgccgacgtc cgcgacggtg tggcccgtat cgaggacctg atgtccgatg agctgggcaa
      901 agccgacgag ttgatggccg aggccgtcca gcacctgttc caggccggtg gcaaacgctt
      961 ccgcccgctg ttcacggtcc tgtcggcgca actcggccct gaaccggacg cctggcaggt
     1021 cacggtggcc ggcgcggtca tcgaactcgt gcacctcgcg acgctgtacc acgacgacgt
     1081 catggacgag gcgcagatgc gccgcggtgc gcagagcgcc aacgcacgct ggggcaacaa
     1141 catcgcgatc ctcgccggcg actacctgtt cgccacggcg tcgcgactcg tgtcccgtct
     1201 gggtcccgac gcggtgcgtg tgattgctga cacatttgct cagctggtca ccggccagat
     1261 gcgggagaca cgcggcgcgg ccgaccacgt cgactccgtc gaccactacc tcaaggtcgt
     1321 ctacgagaag accgcgtgcc tgatcgcggc ctcgggccgg ttcggtgcga cgttctccgg
     1381 cgccaacgac gagcagatcg agcggctgag ccggctcggc ggcatcgtcg gcacggcgtt
     1441 ccagatcgcc gacgacatca tcgacatcga cagcgacccc gacgagtccg gcaagacgcc
     1501 cggcaccgac ctgcgtgaag gcgttcacac cctgccggtg ctgtacgcgc tgcgcgagac
     1561 cggacctgac gccgaccggc tgcgcgaact gctcgccggt cccatcgagc gcgacgagga
     1621 cgtggccgag gccctggcgc tgctgcgcag gtcacctggg ctgaccaagg ccaaggagac
     1681 cgtcgcgtcg tacgcggcgc aggcccgcga agagctttcc cacctgccgg agtgcccggg
     1741 ccgtcaggcg ctcgcggtgc tcgttgacta cacgatcagc cgggaaggca agcttggtgc
     1801 ggccgcgggc aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca
     1861 tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc
     1921 ttacgaaggg acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc
     1981 gtacgatatc ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga
     2041 ggatatccct gactacttca agcagagctt cccggaggga tactcctggg agcggacgat
     2101 gaccttcgaa gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg
     2161 tttcttccag aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca
     2221 gaagaagacc ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct
     2281 ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt
     2341 caagaccacg tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca
     2401 ccgcatcgaa atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc
     2461 tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa
     2521 cctgtatttc caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc
     2581 ctctaaacgg gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc
     2641 acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg
     2701 aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt
     2761 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat
     2821 cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc
     2881 ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg
     2941 tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc
     3001 cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg
     3061 ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa
     3121 atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga
     3181 tcatctctga tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc
     3241 atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc
     3301 atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc
     3361 aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg
     3421 ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg
     3481 cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc
     3541 gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc
     3601 gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg
     3661 cgctgattgc tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga
     3721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3841 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4141 cgccacgctt cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4381 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4441 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac
     4501 ccggggatcc tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg
     4561 ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga
     4621 gggttccgac cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac
     4681 cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt
     4741 ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag
     4801 aagagtcgca caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc
     4861 ctctgaccag cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg
     4921 tgtctgccga ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc
     4981 tctaagtgga aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga
     5041 gttgcaaccc ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg
     5101 ggatcgctga agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac
     5161 ggtgtgaggt actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc
     5221 gcgggcgaga agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag
     5281 aaggcagccg ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc
     5341 gacctcgcag acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg
     5401 gtgctaggtg aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc
     5461 gggatgggta ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg
     5521 atgaacacag cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag
     5581 gcagccgatg agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct
     5641 gagatcttcg agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc
     5701 ggagagctga tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc
     5761 cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc
     5821 gtccggtcga agcgtcctgt gacggttccg cctcacgtcg cggagat
//
