LOCUS       sequence-345904-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0791:Dendra
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     657..676
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        657..675
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2437..2460
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2717..3517
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3642..4230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4131..4150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
       61 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
      121 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
      181 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
      241 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
      301 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
      361 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
      421 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      481 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      541 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      601 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt aaggagtaat
      661 acgactcact atagggagaa ggaggccatt atgacacgta cggtcgccgt catcggcggt
      721 ggcgtcatcg ggctgtccgt cgcgcgacgc gcagcgctcg acgggtggac ggtgcgcctg
      781 cactgcacca ccgagcgcgg cgcgtcgtgg gtggccggcg gcatgctggc gccccacagc
      841 gaaggctggc ccggtgagga gcagttgctc cagctggggc tggaatcgct gcggttgtgg
      901 cactccgggt ttctcgacgg cctgcccgag aaggtggtga ccgcgcggga atcgctcgtg
      961 gtggcggtgg accgcgccga cgccgccgat ttgcgcactg ttgccgagtg gcttgccgcc
     1021 cagggccatc cggtcgagtt caccaccgcc gcccgtgatg tggaaccttt gctggctcaa
     1081 ggtattcggc acggattccg cgcgacgaca gagctggcgg tggacaaccg cggggtggtc
     1141 gacgcgctgg ccgcgcactg cgagcggttg gccgtgcggt gggcggggca ggtcgacgac
     1201 ctcgccgatg tcgacgccga cgccgtggtg atcgccaacg gcatcgacgc gccgcggctg
     1261 tggccggacc tgccggtacg cccggtcaag ggtgaggtgc tgcggctgcg gtggcgcaag
     1321 ggttgtatgc cggtgccgca gcgcgtgatc cgcgcccgtg tgcacggcag gcaggtgtac
     1381 ctggtgcccc gcgccgacgg cgtggtggtc ggtgccacgc agtacgagca cggccgcgac
     1441 accgcaccgg tggtcagcgg cgtgcgggat ctgctggagg acgcctgcgc ggtgatgccc
     1501 gcgctggggg agtacgaact ggccgagatc gcggccgggc tgcggccgat gacgccggac
     1561 ggtttgcccg tcgtggaacg tgtcgacgag cgcaccgtgg tcgccgtcgg gcacggacgg
     1621 aacggatttc tgttggcgcc gtggaccgct gagcgggtcg cggcagaact cgaagtgggt
     1681 gtgggagcga aaaagcttgg tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag
     1741 gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga acggtcacgc cttcgtgatc
     1801 gagggggagg gtaagggtaa gccttacgaa gggacccaga ccgccaacct caccgtcaag
     1861 gagggcgccc ctctcccatt ctcgtacgat atcctcacta ccgcggtgca ttacgggaac
     1921 cgcgtgttca ccaagtaccc cgaggatatc cctgactact tcaagcagag cttcccggag
     1981 ggatactcct gggagcggac gatgaccttc gaagacaagg gtatctgtac gatccgcagc
     2041 gacatctcgc tggaagggga ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt
     2101 ccgccgaacg gcccggtcat gcagaagaag accctcaagt gggagccgag cacggagaag
     2161 ctgcacgttc gggacggcct cctggtcggc aacatcaaca tggcgctgct gctggaaggc
     2221 ggcggccact acctatgcga cttcaagacc acgtacaaag cgaagaaggt cgtccagctg
     2281 cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg ggaacgattc ggactacaac
     2341 aaggtcaagt tgtacgagca cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg
     2401 ttcgaaggcg gcggcgggga gaacctgtat ttccaggact acaaggacga cgacgacaag
     2461 tgatttaaat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gttaactagc
     2521 gtacgatcga attcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg
     2581 gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc
     2641 atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc
     2701 gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg
     2761 aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct
     2821 ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga
     2881 ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct
     2941 ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc
     3001 tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc
     3061 agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac cttactcgcc
     3121 tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg
     3181 acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt
     3241 gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa
     3301 tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca
     3361 gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc
     3421 gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg
     3481 atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgagc ggagaacgag
     3541 atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacggggga tccactagtt
     3601 ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3781 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4021 ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc ggacaggtat
     4081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4261 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4321 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4381 cgcaacgcgt gcggccgcgg tacccgggga tcctctagag tcgaccacca agggcaccat
     4441 ctctgcttgg gccaccccgt tggccgcagc cagctcgctg agagccgtga acgacagggc
     4501 gaacgccagc ccgccgacgg cgagggttcc gaccgctgca actcccggtg caaccttgtc
     4561 ccggtctatt ctcttcactg caccagctcc aatctggtgt gaatgcccct cgtctgttcg
     4621 cgcaggcggg gggctctatt cgtttgtcag catcgaaagt agccagatca gggatgcgtt
     4681 gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt tgcagacccc tggaaagaaa
     4741 aatggccaga gggcgaaaac accctctgac cagcggagcg ggcgacggga atcgaacccg
     4801 cgtagctagt ttggaagaat gggtgtctgc cgaccacata tgggccggtc aagataggtt
     4861 tttaccccct ctcggctgca tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt
     4921 tgaagcctga gagttgcaca ggagttgcaa cccggtagcc ttgttcacga cgagaggaga
     4981 cctagttggc acgtcgcgga tggggatcgc tgaagactca gcgcagcggg aggatccaag
     5041 cctcatacgt caacccgcag gacggtgtga ggtactacgc gctgcagacc tacgacaaca
     5101 agatggacgc cgaagcctgg ctcgcgggcg agaagcggct catcgagatg gagacctgga
     5161 cccctccaca ggaccgggcg aagaaggcag ccgccagcgc catcacgctg gaggagtaca
     5221 cccggaagtg gctcgtggag cgcgacctcg cagacggcac cagggatctg tacagcgggc
     5281 acgcggagcg ccgcatctac ccggtgctag gtgaagtggc ggtcacagag atgacgccag
     5341 ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca cccgactgcc cgccggcatg
     5401 cctacaacgt cctccgggcg gtgatgaaca cagcggtcga ggacaagctg atcgcagaga
     5461 acccgtgccg gatcgagcag aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg
     5521 aggagctgga catcgtcgcc gctgagatct tcgagcacta ccggatcgcg gcatacatcc
     5581 tggcgtggac gagcctccgg ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg
     5641 acgacggcat gacgatgaag ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga
     5701 tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg
     5761 tcgcggagat gatccgagcg cacatgaagg accgtacgaa gatgaacaag ggccccgagg
     5821 cattcctggt gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc
     5881 tg
//
