LOCUS       sequence-345905-        5405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1364:Dendra
FEATURES             Location/Qualifiers
     source          1..5405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1949..1972
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2229..3029
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3154..3742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3643..3662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt
       61 cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc
      121 taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc
      181 gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga
      241 gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca
      301 gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg
      361 ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa
      421 tattggatcg tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc
      481 ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg
      541 ttcgcacgca caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
      601 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
      661 tagggagaag gaggccatta tggccaaggc tgacaaggcc acggcggttg ccgacattgc
      721 cgagcagttc aaggcgtcga cggccaccgt cgtcaccgag taccgcgggc tgactgtggc
      781 caacctggct gagctgcgtc gtgccctcgg cgactccgcc acgtacaccg tcgccaagaa
      841 cactctggtg aagcgcgccg cttcggaagc cggcattgaa ggcctcgacg agctgttcgc
      901 cggtcccacg gcgatcgcgt tcgtcaaggg cgaagcggtc gatgccgcca aggcgatcaa
      961 gaagttcgcc aaggacaaca aggcgctcgt catcaagggc ggctacatgg acggcaaggc
     1021 gctgtccgtc gccgacgtcg agaagatcgc cgacctggag tcccgcgagg tgctgctggc
     1081 caagctggca ggcgccatga agggcaacct gtcgaaggct gccggtctgt tcaacgcgcc
     1141 cgcttctcag gtggcccggc tcgctgccgc gctgcaggag aagaaggccg gcgaagaagc
     1201 cgcaaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
     1261 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
     1321 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
     1381 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
     1441 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
     1501 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
     1561 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
     1621 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     1681 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     1741 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     1801 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     1861 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     1921 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     1981 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     2041 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     2101 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     2161 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     2221 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     2281 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     2341 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     2401 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     2461 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     2521 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     2581 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     2641 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     2701 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     2761 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     2821 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     2881 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     2941 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     3001 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     3061 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     3121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3301 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3541 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     3601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     3901 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     3961 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     4021 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     4081 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     4141 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     4201 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     4261 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     4321 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     4381 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     4441 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     4501 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     4561 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     4621 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     4681 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     4741 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     4801 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     4861 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     4921 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     4981 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     5041 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     5101 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     5161 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     5221 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     5281 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     5341 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     5401 ggcta
//
