LOCUS       sequence-345906-        6107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1132:Dendra
FEATURES             Location/Qualifiers
     source          1..6107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2651..2674
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2931..3731
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3856..4444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4345..4364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt
       61 cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc
      121 taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc
      181 gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga
      241 gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca
      301 gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg
      361 ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa
      421 tattggatcg tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc
      481 ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg
      541 ttcgcacgca caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
      601 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
      661 tagggagaag gaggccatta tgaacacccg agcggatgtg gtcgtcgtcg gagccggacc
      721 ggctggttcg gctgccgcgg catgggccgc acgcgcaggt cgcgacgtgg tcgtggtcga
      781 cgccgcgcag tttccccgtg acaaggcgtg cggggacggg ctgacgccgc gtgccgtggc
      841 cgaactgcag cgcctcggga tggcttcgtg gctggacacc cgcatccgcc accacgggct
      901 gcgcatgtcg gggttcggcg ccgacgtcga gattccgtgg ccgggtccgt cattccccgc
      961 gacaagcagc gcggtgccgc gtaccgaact cgacgaccgg atccgctcgg tcgccgccga
     1021 cgacggcgcc aagatgatgc tgggcaccaa agtcgttgat gtgacccatg attcgagcgg
     1081 ccgcgtggac gccgtggtgc tcgacgacgg caacaccgtc ggttgcgcgc agctcatcgt
     1141 ggccgacggc gcgcgttcga cgctgggccg cgtgctgggc cgtacctggc acagggagac
     1201 cgtgtacggc gtggcgatcc gcggctacat cgcgagcccc cgcgcgtccg agccctggat
     1261 cacgtcgcac ctcgaactgc gctcccccga gggcaaggtg ctacccgggt acggctggat
     1321 gttcccgctg ggcaacggcg aggtgaacat cggcgtcggc gcgcttgcga ccgcgaagcg
     1381 tcctgccgat gcggccctgc ggccactgat gtcctattac gccgacctgc gccgcgagga
     1441 gtggggtctg gtcggcgaac cgcgcgccgg actgtcggcg ctgctgccga tgggtggcgc
     1501 ggtctcgggc gtcgccgggc ccaactggat gctgatcggc gacgccgcgg catgcgtcaa
     1561 cccgctcaac ggcgagggca tcgactacgg gctggaaaca ggccggctgg ccgccgaact
     1621 catgacctcc ggcggcgtga ccgactactc ctcggcctgg ccgacgctgc tgcaggagca
     1681 ctacgcgcgc gggttctcgg tggcgcgcag gctcgcgttg ctgctgacgc tgccgcgctt
     1741 cctgcaggtc accgggcctg tggcgatgcg gtcggcgacg ttgatgacca tcgcggtccg
     1801 ggtgatgggc aacctggtga ccgacgagga cgccgactgg atcgctcgcg tgtggcgcac
     1861 cgcgggcctg gcgtcgcggc ggatcgatca gcgggtgccg ttcagcaagc ttggtgcggc
     1921 cgcgggcaac acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat
     1981 ggagggcaac gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta
     2041 cgaagggacc cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta
     2101 cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga
     2161 tatccctgac tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac
     2221 cttcgaagac aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt
     2281 cttccagaac gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa
     2341 gaagaccctc aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt
     2401 cggcaacatc aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa
     2461 gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg
     2521 catcgaaatc ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt
     2581 ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct
     2641 gtatttccag gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc
     2701 taaacgggtc ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg
     2761 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     2821 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     2881 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
     2941 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
     3001 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     3061 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg
     3121 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     3181 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     3241 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     3301 tctctgatcc attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg
     3361 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     3421 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     3481 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     3541 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     3601 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     3661 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     3721 agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc
     3781 tgattgctgg ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa
     3841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3961 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4261 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4561 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg
     4621 gggatcctct agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg
     4681 cagccagctc gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg
     4741 ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag
     4801 ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg
     4861 tcagcatcga aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag
     4921 agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc
     4981 tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt
     5041 ctgccgacca catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
     5101 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
     5161 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
     5221 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
     5281 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
     5341 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
     5401 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
     5461 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
     5521 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
     5581 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
     5641 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
     5701 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
     5761 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
     5821 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
     5881 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
     5941 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     6001 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     6061 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggcta
//
