LOCUS       sequence-345909-        5555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0937:Dendra
FEATURES             Location/Qualifiers
     source          1..5555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     727..746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        727..745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2180..2203
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2460..3260
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3385..3973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3874..3893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac
       61 caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct
      121 ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc
      181 ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg
      241 cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag
      301 cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac
      361 cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg
      421 ccgccgccag gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg
      481 ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc
      541 acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc
      601 atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg
      661 agcgggagaa ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt
      721 aaggagtaat acgactcact atagggagaa ggaggccatt atgttgatcg ttgaagacga
      781 ggaatcgctg gccgatccct tggcgtttct gctccgtaag gagggcttcg aggccaccgt
      841 ggtgggcgac ggcccgtcgg ccctcgcgga gttcgaacgc tccggcgccg acatcgtgct
      901 gctggatctc atgctccccg gcatgagtgg caccgacgtg tgcaagcagt tgcgtgcgcg
      961 gtcaagcgtc cccgtcatca tggtgacggc gcgcgacagc gagatcgaca aggtggtcgg
     1021 cctcgagctc ggtgctgacg actatgtcac caagccgtac tcggcgcgtg aactcatcgc
     1081 ccggattcgt gcggtgttgc gccgtggcgc cgacaacgac gacgcgggcg ccgacgacgg
     1141 cgtgctggag gccggtcccg tgcgcatgga cgtcgaacga cacgtggtga gcgtcaacgg
     1201 tgaaccgatc acgttgccgc tcaaggagtt cgacctgctc gagtacctga tgcgcaacag
     1261 tggtcgcgtg ctcacccgcg gtcagctcat cgaccgggtg tggggtgcgg actacgtcgg
     1321 cgacaccaaa acccttgacg tgcacgtcaa aaggctgcgg tcgaagatcg aggaggatcc
     1381 ggccaacccg gtgcacctgg tgaccgtacg cggcctggga tacaagctgg agggcaagct
     1441 tggtgcggcc gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa
     1501 ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg
     1561 taagccttac gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc
     1621 attctcgtac gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta
     1681 ccccgaggat atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg
     1741 gacgatgacc ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg
     1801 ggactgtttc ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt
     1861 catgcagaag aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg
     1921 cctcctggtc ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg
     1981 cgacttcaag accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt
     2041 ggaccaccgc atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga
     2101 gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg
     2161 ggagaacctg tatttccagg actacaagga cgacgacgac aagtgattta aataacccct
     2221 tggggcctct aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg
     2281 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
     2341 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
     2401 ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga
     2461 tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg
     2521 gtcagcttct caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc
     2581 gtagcgtccg gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc
     2641 tgggtccggg agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg
     2701 atcctgccac gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc
     2761 tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag
     2821 agcagatcat ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc
     2881 gtgtccatga actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga
     2941 cgctgcatct tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca
     3001 ttcttcagga tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc
     3061 gctttgcctt ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg
     3121 gtcatgcctt tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac
     3181 tgggccgaga tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg
     3241 ccgctcgcca gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca
     3301 aggtcgcgct gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc
     3361 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3421 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3481 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     3541 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3601 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3661 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3721 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3781 agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt
     3841 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3901 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3961 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4021 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4081 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg
     4141 cggtacccgg ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc
     4201 cgttggccgc agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga
     4261 cggcgagggt tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca
     4321 ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct
     4381 attcgtttgt cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca
     4441 ggtcagaaga gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa
     4501 aacaccctct gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag
     4561 aatgggtgtc tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct
     4621 gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc
     4681 acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc
     4741 ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg
     4801 caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc
     4861 tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg
     4921 gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg
     4981 gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc
     5041 tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg
     5101 tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg
     5161 gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag
     5221 cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc
     5281 gccgctgaga tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc
     5341 cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg
     5401 aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc
     5461 aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga
     5521 gcgcacatga aggaccgtac gaagatgaac aaggg
//
