LOCUS       sequence-345912-        5249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1067:Dendra
FEATURES             Location/Qualifiers
     source          1..5249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     741..760
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        741..759
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1888..1911
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2168..2968
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3093..3681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3582..3601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
       61 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
      121 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
      181 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
      241 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
      301 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
      361 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
      421 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
      481 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
      541 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
      601 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
      661 aagggtctct gacgagcggg agaactccct atcagtgata gagtttgtcc tccctatcag
      721 tgatagatag gcttaaggag taatacgact cactataggg agaaggaggc cattatggag
      781 atcctggcca gccggatgtt gatccgaccg gtcgactacg agaaatcgct cgagttctac
      841 cgcgacggga tcggcctggc gatcgcgcgt gaataccccg gcggcacagt gttctacgcc
      901 ggtcagtcgc tgatcgagat cgccggccac ggcgaaccgt cgggaggcgg cagcctgtgg
      961 ttgcaggtgc gcgacatcta cgccgcgcag caggagctcg agggtcgcgg ggtgccgatc
     1021 acgcggccgg ccaaacagga accgtggggt ctgcacgaga tgcacgtgac cgaccccgac
     1081 ggcatcacgc tgatcttcgt ccaggtgccc tcgacgcatc cgctgcggcg cgacgtgcgg
     1141 gataagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa ggaggacatg
     1201 cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat cgagggggag
     1261 ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa ggagggcgcc
     1321 cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa ccgcgtgttc
     1381 accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga gggatactcc
     1441 tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag cgacatctcg
     1501 ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac
     1561 ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa gctgcacgtt
     1621 cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg cggcggccac
     1681 tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg
     1741 cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa caaggtcaag
     1801 ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc
     1861 ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa gtgatttaaa
     1921 taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag cgtacgatcg
     1981 aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga
     2041 atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa
     2101 aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga
     2161 tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga
     2221 gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag
     2281 ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg
     2341 tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga
     2401 gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt
     2461 ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc
     2521 acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc ctgcaagccc
     2581 ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc
     2641 caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca
     2701 ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg
     2761 ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg
     2821 tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct
     2881 gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga
     2941 atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga gatgacgttg
     3001 gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt tccatgagcg
     3061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3481 gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc
     3541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg
     3841 tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca tctctgcttg
     3901 ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg cgaacgccag
     3961 cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt cccggtctat
     4021 tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg
     4081 ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg
     4141 tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa aaatggccag
     4201 agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc gcgtagctag
     4261 tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt ttttaccccc
     4321 tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg
     4381 agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag acctagttgg
     4441 cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa gcctcatacg
     4501 tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac aagatggacg
     4561 ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg acccctccac
     4621 aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac acccggaagt
     4681 ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg cacgcggagc
     4741 gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc
     4801 gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat gcctacaacg
     4861 tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag aacccgtgcc
     4921 ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct gaggagctgg
     4981 acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc ctggcgtgga
     5041 cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca
     5101 tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg
     5161 gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga
     5221 tgatccgagc gcacatgaag gaccgtacg
//
