LOCUS       sequence-34592-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Stenoien DL, Mielke M, Mancini MA
  TITLE     Intranuclear ataxin1 inclusions contain both fast- and 
            slow-exchanging components.
  JOURNAL   Nat Cell Biol. 2002 Oct . 4(10):806-10.
  PUBMED    12360291
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Oct . 4(10):806-10."
COMMENT     Sequence Label: pEYFP-C3-HA-Ubiquitin
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..52
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             62..289
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    451..572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(488..507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     542..561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(716..737)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcagatctc gagctcaagc ttatgtaccc atacgatgtt ccggattacg ctagcggatc
       61 catgcagatc ttcgtgaaga cccttaccgg caagaccatc acccttgagg tggagcccag
      121 tgacaccatc gaaaatgtga aggccaagat ccaggataaa gaaggcatcc ctcccgacca
      181 gcagaggctc atctttgcag gcaagcagct ggaagatggc cgcactcttt ctgactacaa
      241 catccagaag gagtcgaccc tgcacctggt cctgcgtctc agaggtgggt gaattctgca
      301 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
      361 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      421 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      541 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
      601 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      661 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      721 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
      781 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagtttt
//
