LOCUS       sequence-345936-        2151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric version of the genetically encoded voltage indicator 
            ArcLight-Q239..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ruhl P, Langner JM, Reidel J, Schonherr R, Hoshi T, Heinemann SH
  TITLE     Monitoring of compound resting membrane potentials of cell cultures 
            with ratiometric genetically encoded voltage indicators.
  JOURNAL   Commun Biol. 2021 Oct 7;4(1):1164. doi: 10.1038/s42003-021-02675-0.
  PUBMED    34620975
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-10-7"; volume: "4(1):1164. doi"; pages: " 
            10.1038/s42003-021-02675-0"
COMMENT     Sequence Label: rArc
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1435..2151
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(1465..1493)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2088..2108
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgcacatgg tcagcgagct gatcaaggaa aacatgcaca tgaaactgta catggagggg
       61 actgtgaaca atcaccattt caaatgcacc tccgagggcg aagggaagcc ctacgagggc
      121 acacagacta tgaggatcaa ggcagtggag ggaggaccac tgccattcgc ctttgacatt
      181 ctggctacct cattcatgta cggcagcaaa accttcatca atcacactca ggggattccc
      241 gacttcttta agcagtcttt ccctgaaggc tttacttggg agcgagtgac cacatacgag
      301 gatggaggcg tcctgaccgc cacacaggac acaagtctgc aggatggctg tctgatctat
      361 aacgtgaaga ttcgcggggt caactttccc agtaatggac ctgtgatgca gaagaaaacc
      421 ctgggatggg aggcttcaac tgaaaccctg tacccagcag acggaggact ggagggacga
      481 gcagatatgg ctctgaaact ggtgggcggg ggacacctga tctgcaacct gaagactacc
      541 tatcggtcca agaaacctgc taagaatctg aaaatgccag gcgtgtacta tgtggaccgg
      601 agactggaga gaattaagga agcagataaa gagacctacg tggagcagca cgaagtggct
      661 gtcgcacgat attgtgacct gccttctaaa ctgggccatc gggaattcat ggagggattc
      721 gacggttcag attttagtcc tccagctgat ttagttggcg ttgacggtgc agtcatgcgg
      781 aacgtcgttg acgtcacgat aaatggtgac gtcactgctc cgccgaaagc tgcgccaaga
      841 aaaagtgaat cggtaaagaa agttcattgg aatgatgtag accaaggacc gagtgaaaaa
      901 ccagagacaa gacaggagga acgaatagat atacccgaga tttcaggtct atggtggggc
      961 gagaatgaac atggagtgga cgatgggaga atggagatac ctactactgg tgtaggtcgc
     1021 gtccagtttc gtgtccgagc agtgattgat catctaggga tgcgagtctt tggagtcttc
     1081 ctaattttct tggacatcat cctcatgatc attgatctca gtcttccagg aaaaagtgaa
     1141 tcttcacaat ccttttatga cgggatggct ttggctcttt cttgttattt catgctggat
     1201 ttaggattaa ggatatttgc ctacgggccc aagaatttct tcaccaaccc ctgggaggtt
     1261 gctgatggtt tgattatcgt tgtcacattc gtcgtcacga tattttacac tgtgttagat
     1321 gaatacgtgc aagaaacagg agccgatggt ttggggcaat tggttgtgtt ggcccgtttg
     1381 ctgcgtgtgg ttcgattagc aagaatattt tattcccacc aacaagggga tcccatgagt
     1441 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1501 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1561 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1621 actttaactt atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac
     1681 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1741 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1801 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1861 tacaactata acgatcacca ggtgtacatc atggcagaca aacaaaagaa tggaatcaaa
     1921 gctaacttca aaattagaca caacattgaa gatggaggcg ttcaactagc agaccattat
     1981 caacaaaata ctccaattgg cgatgggccc gtccttttac cagacaacca ttacctgttt
     2041 acaacttcta ctctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     2101 tttgtaacag ctgatgggat tacacatggc atggatgaac tatacaaatg a
//
