LOCUS       sequence-345958-        9844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Lopez C, Cao M, Yao Z, Shao Z
  TITLE     Revisiting the unique structure of autonomously replicating 
            sequences in Yarrowia lipolytica and its role in pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2021 Aug 6. pii: 
            10.1007/s00253-021-11399-4. doi: 10.1007/s00253-021-11399-4.
  PUBMED    34357429
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2021 Aug 6. pii: 10.1007/s00253-021-11399-4. 
            doi: 10.1007/s00253-021-11399-4."
COMMENT     Sequence Label: pSCARS1
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..27
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     255..271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..300
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1362..1378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4509..5225
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGNQ
                     LVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYED
                     GGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVGQ
                     VILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAIA
                     QLTSLGKPLGSLHEWV"
     terminator      5232..5479
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5248..5266)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             join(5504..5572,5573..6364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGEHGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5722..5741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6535..7123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7024..7043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7277..7294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7411..7432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7447..7452,7453..7470,7471..7477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7485..7501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7490..7512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7509..7525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7509..7525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(7542..7646)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7683..8186
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8445..8725
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8726..9400
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(join(9503..9844,1..114))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9640..9661)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
       61 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
      121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      181 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      241 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
      301 cgaattgggt acatcgatca tactactgta tattcaagca agtatatccg tagggtgcgg
      361 gtgatttgga tctaaggttc gtactcaaca ctcacgagca gcttgcctat gttacatcct
      421 tttatcagac ataacataat tggagtttac ttacacacgg ggtgtacctg tatgagcacc
      481 acctacaatt gtagcactgg tacttgtaca aagaatttat tcgtacgaat cacaggagcg
      541 gccgccctca ccgaaccagc gaatacctca gcggtcccct gcagtgactc aacaaagcga
      601 tatgaacatc ttgcgatggt atcctgctga tagtttttac tgtacaaaca cctgtgtagc
      661 tccttctagc atttttaagt tattcacacc tcaaggggag ggataaatta aataaattcc
      721 aaaagcgaag atcgagaaac taaattaaaa ttccaaaaac gaagttggaa cacaaccccc
      781 cgaaaaaaaa caacaaacaa aaaacccaac aaaataaaca aaaacaaaat aaatatataa
      841 ctaccagtat ctgactaaaa gttcaaatac tcgtacttaa aacaaataga aatgagccgg
      901 ccaaaattct gcagaaaaaa aattcaaaca agtactggta taattaaatt aaaaaacaca
      961 tcaaagtatc ataacgttag ttattttatt ttatttaata aaagaaaaca acaaaatagg
     1021 ctcaaaactt tcaacttata cgatacatac caaataacaa tttagtattt atctaagtgc
     1081 ttttcgtaga taatggaata caaatggata tccagagtat acacatggat agtatacact
     1141 gacacgacaa ttctgtatct ctttatgtta actactgtga ggcgttaaat agagcttgat
     1201 atataaaatg ttacatttca cagtctgaac ttttgcagat tacctaattt ggtaagatat
     1261 taattatgaa ctgaaagttg atggcatccc taaatttgat gaaagatgaa attgtaaatg
     1321 agggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
     1381 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1441 ttcgccagct ggcgtaatag cgaagaggcg gccgcgccaa actgatctca agactttatt
     1501 gaaatcagca acaccgattc tcaatgaagg cacatacttc ttctgcaaca ttcacttgac
     1561 gcctaaagtt ggtgagaaat ggaccgacaa gacatattct gctatccacg gactgttgcc
     1621 tgtgtcggtg gctacaatac gtgagtcaga agggctgacg gtggtggttc ccaaggaaaa
     1681 ggtcgacgag tatctgtctg actcgtcatt gccgcctttg gagtacgact ccaactatga
     1741 gtgtgcttgg atcactttga cgatacattc ttcgttggag gctgtgggtc tgacagctgc
     1801 gttttcggcg cggttggccg acaacaatat cagctgcaac gtcattgctg gctttcatca
     1861 tgatcacatt tttgtcggca aaggcgacgc ccagagagcc attgacgttc tttctaattt
     1921 ggaccgatag ccgtatagtc cagtctatct ataagttcaa ctaactcgta actattacca
     1981 taacatatac ttcactgccc cagataaggt tccgataaaa agttctgcag actaaattta
     2041 tttcagtctc ctcttcacca ccaaaatgcc ctcctacgaa gctcgagcta acgtccacaa
     2101 gtccgccttt gccgctcgag tgctcaagct cgtggcagcc aagaaaacca acctgtgtgc
     2161 ttctctggat gttaccacca ccaaggagct cattgagctt gccgataagg tcggacctta
     2221 tgtgtgcatg atcaagaccc atatcgacat cattgacgac ttcacctacg ccggcactgt
     2281 gctccccctc aaggaacttg ctcttaagca cggtttcttc ctgttcgagg acagaaagtt
     2341 cgcagatatt ggcaacactg tcaagcacca gtacaagaac ggtgtctacc gaatcgccga
     2401 gtggtccgat atcaccaacg cccacggtgt acccggaacc ggaatcattg ctggcctgcg
     2461 agctggtgcc gaggaaactg tctctgaaca gaagaaggag gacgtctctg actacgagaa
     2521 ctcccagtac aaggagttcc tggtcccctc tcccaacgag aagctggcca gaggtctgct
     2581 catgctggcc gagctgtctt gcaagggctc tctggccact ggcgagtact ccaagcagac
     2641 cattgagctt gcccgatccg accccgagtt tgtggttggc ttcattgccc agaaccgacc
     2701 taagggcgac tctgaggact ggcttattct gacccccggg gtgggtcttg acgacaaggg
     2761 agacgctctc ggacagcagt accgaactgt tgaggatgtc atgtctaccg gaacggatat
     2821 cataattgtc ggccgaggtc tgtacggcca gaaccgagat cctattgagg aggccaagcg
     2881 ataccagaag gctggctggg aggcttacca gaagattaac tgttagaggt tagactatgg
     2941 atatgtaatt taactgtgta tatagagagc gtgcaagtat ggagcgcttg ttcagcttgt
     3001 atgatggtca gacgacctgt ctgatcgagt atgtatgata ctgcacaacc tgtgtatccg
     3061 catgatctgt ccaatggggc atgttgttgt gtttctcgat acggagatgc tgggtacaag
     3121 tagctaatac gattgaacta cttatactta tatgaggctt gaagaaagct gacttgtgta
     3181 tgacttattc tcaactacat ccccagtcac aataccacca ctgcactacc actacaccaa
     3241 aaccatgatc aaaccaccca tggacttcct ggaggcagaa gaacttgtta tggaaaagct
     3301 caagagagag aagccaagat actatcaaga catgtgtcgc aacttcaagg aggaccaagc
     3361 tctgtacacc gagaaacagg cctttgtcga cgaagctcta aagctgaaac gggacatcga
     3421 aaagttggag aagtcaatct ggaaactaca cgtagaaaac agacaccatc tccaacaagt
     3481 acaggacttc aaatttgcac tgaagacatt ctgcggccgc accgatcgcc cttcccaaca
     3541 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     3601 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3661 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3781 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3961 ataaccctga tttaaataga gaccgggttg gcggcgcatt tgtgtcccaa aaaacagccc
     4021 caattgcccc aattgacccc aaattgaccc agtagcgggc ccaaccccgg cgagagcccc
     4081 cttctcccca catatcaaac ctcccccggt tcccacactt gccgttaagg gcgtagggta
     4141 ctgcagtctg gaatctacgc ttgttcagac tttgtactag tttctttgtc tggccatccg
     4201 ggtaacccat gccggacgca aaatagacta ctgaaaattt ttttgctttg tggttgggac
     4261 tttagccaag ggtataaaag accaccgtcc ccgaattacc tttcctcttc ttttctctct
     4321 ctccttgtca actcacaccc gaaatcgtta agcatttcct tctgagtata agaatcattc
     4381 aaaatggtga gtttcagagg cagcagcaat tgccacgggc tttgagcaca cggccgggtg
     4441 tggtcccatt cccatcgaca caagacgcca cgtcatccga ccagcacttt ttgcagtact
     4501 aaccgcaggt gagcaagcag atcctgaaga acaccggcct gcaggagatc atgagcttca
     4561 aggtgaacct ggagggcgtg gtgaacaacc acgtgttcac catggagggc tgcggcaagg
     4621 gcaacatcct gttcggcaac cagctggtgc agatccgcgt gaccaagggc gcccccctgc
     4681 ccttcgcctt cgacatcctg agccccgcct tccagtacgg caaccgcacc ttcaccaagt
     4741 accccgagga catcagcgac ttcttcatcc agagcttccc cgccggcttc gtgtacgagc
     4801 gcaccctgcg ctacgaggac ggcggcctgg tggagatccg cagcgacatc aacctgatcg
     4861 aggagatgtt cgtgtaccgc gtggagtaca agggccgcaa cttccccaac gacggccccg
     4921 tgatgaagaa gaccatcacc ggcctgcagc ccagcttcga ggtggtgtac atgaacgacg
     4981 gcgtgctggt gggccaggtg atcctggtgt accgcctgaa cagcggcaag ttctacagct
     5041 gccacatgcg caccctgatg aagagcaagg gcgtggtgaa ggacttcccc gagtaccact
     5101 tcatccagca ccgcctggag aagacctacg tggaggacgg cggcttcgtg gagcagcacg
     5161 agaccgccat cgcccagctg accagcctgg gcaagcccct gggcagcctg cacgagtggg
     5221 tgtaaccgcg gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc
     5281 cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt
     5341 tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta
     5401 cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg
     5461 ctcgaaggct ttaatttgcg gccaatattg aaaaaggaag agtatgagta ttcaacattt
     5521 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5581 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5641 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5701 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5761 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5821 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5881 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5941 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6001 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6061 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6121 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6181 ctggtttatt gctgataaat ctggagccgg tgagcatggg tctcgcggta tcattgcagc
     6241 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6301 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6361 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6421 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atctaaggat cttcttgaga
     6541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6661 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7201 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7261 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7321 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7381 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7441 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7501 atttcacaca ggaaacagct atgaccatga ttacgaattc tactcttcct ttttcaatat
     7561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt
     7681 tcatcacgtg ctataaaaat aattataatt taaatttttt aatataaata tataaattaa
     7741 aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa
     7801 agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt
     7861 attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat
     7921 tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa
     7981 taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt
     8041 ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac
     8101 ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg
     8161 cgcgtgtaag ttacaggcaa gcgatccgtc taagaaacca ttattatcat gacattaacc
     8221 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     8281 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     8341 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     8401 tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcacgaca ttactatata
     8461 tataatatag gaagcattta atagaacagc atcgtaatat atgtgtactt tgcagttatg
     8521 acgccagatg gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta
     8581 caatcttgat ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag
     8641 aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat
     8701 taagcacaca aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc
     8761 attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga
     8821 tgctgacttg ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat
     8881 tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata
     8941 cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta
     9001 cggcattgat atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct
     9061 cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc
     9121 agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg
     9181 tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag
     9241 cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt
     9301 aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc
     9361 taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt
     9421 atttaagtat tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt
     9481 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     9541 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     9601 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     9661 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     9721 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     9781 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     9841 gcga
//
